LOCUS       sequence-237975-        9024 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-to-T base editor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9024)
  AUTHORS   Huang TP, Zhao KT, Miller SM, Gaudelli NM, Oakes BL, Fellmann C, 
            Savage DF, Liu DR
  TITLE     Circularly permuted and PAM-modified Cas9 variants broaden the 
            targeting scope of base editors.
  JOURNAL   Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 
            10.1038/s41587-019-0134-y.
  PUBMED    31110355
REFERENCE   2  (bases 1 to 9024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 
            10.1038/s41587-019-0134-y."
COMMENT     Sequence Label: CP1249-CBEmax
FEATURES             Location/Qualifiers
     source          1..9024
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     15..34
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        15..33
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             83..103
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             104..787
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="SSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWGG
                     RHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFLS
                     RYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSNE
                     AHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHIL
                     WATGLK"
     CDS             1304..3022
                     /codon_start=1
                     /product="N-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(N)
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE"
     CDS             5078..5326
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5357..5605
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5648..5668
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5677..5694
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5717..5734)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5723..5947
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6018..6034)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6018..6034)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6031..6053)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6042..6058
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6066..6072,6073..6090,6091..6096))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6111..6132
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6249..6266)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6420..7008)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6500..6519)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7179..7970,7971..8039))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7802..7821
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8040..8144)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8223..8242)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8414..8793
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8794..8997
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8948..8968
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 agatccgcgg ccgctaatac gactcactat agggagagcc gccaccatga aacggacagc
       61 cgacggaagc gagttcgagt caccaaagaa gaagcggaaa gtctcctcag agactgggcc
      121 tgtcgccgtc gatccaaccc tgcgccgccg gattgaacct cacgagtttg aagtgttctt
      181 tgacccccgg gagctgagaa aggagacatg cctgctgtac gagatcaact ggggaggcag
      241 gcactccatc tggaggcaca cctctcagaa cacaaataag cacgtggagg tgaacttcat
      301 cgagaagttt accacagagc ggtacttctg ccccaatacc agatgtagca tcacatggtt
      361 tctgagctgg tccccttgcg gagagtgtag cagggccatc accgagttcc tgtccagata
      421 tccacacgtg acactgttta tctacatcgc caggctgtat caccacgcag acccaaggaa
      481 taggcagggc ctgcgcgatc tgatcagctc cggcgtgacc atccagatca tgacagagca
      541 ggagtccggc tactgctggc ggaacttcgt gaattattct cctagcaacg aggcccactg
      601 gcctaggtac ccacacctgt gggtgcgcct gtacgtgctg gagctgtatt gcatcatcct
      661 gggcctgccc ccttgtctga atatcctgcg gagaaagcag ccccagctga ccttctttac
      721 aatcgccctg cagtcttgtc actatcagag gctgccaccc cacatcctgt gggccacagg
      781 cctgaagtct ggaggatcta gcggaggatc ctctggcagc gagacaccag gaacaagcga
      841 gtcagcaaca ccagagagca gtggcggcag cagcggcggc agccccgagg ataatgagca
      901 gaaacagctg tttgtggaac agcacaagca ctacctggac gagatcatcg agcagatcag
      961 cgagttctcc aagagagtga tcctggccga cgctaatctg gacaaagtgc tgtccgccta
     1021 caacaagcac cgggataagc ccatcagaga gcaggccgag aatatcatcc acctgtttac
     1081 cctgaccaat ctgggagccc ctgccgcctt caagtacttt gacaccacca tcgaccggaa
     1141 gaggtacacc agcaccaaag aggtgctgga cgccaccctg atccaccaga gcatcaccgg
     1201 cctgtacgag acacggatcg acctgtctca gctgggaggc gacggcggat caggaggctc
     1261 tggcggttca ggtggatcag gcggtagcgg aggttcaggt ggtatggaca agaagtacag
     1321 catcggcctg gccatcggca ccaactctgt gggctgggcc gtgatcaccg acgagtacaa
     1381 ggtgcccagc aagaaattca aggtgctggg caacaccgac cggcacagca tcaagaagaa
     1441 cctgatcgga gccctgctgt tcgacagcgg cgaaacagcc gaggccaccc ggctgaagag
     1501 aaccgccaga agaagataca ccagacggaa gaaccggatc tgctatctgc aagagatctt
     1561 cagcaacgag atggccaagg tggacgacag cttcttccac agactggaag agtccttcct
     1621 ggtggaagag gataagaagc acgagcggca ccccatcttc ggcaacatcg tggacgaggt
     1681 ggcctaccac gagaagtacc ccaccatcta ccacctgaga aagaaactgg tggacagcac
     1741 cgacaaggcc gacctgcggc tgatctatct ggccctggcc cacatgatca agttccgggg
     1801 ccacttcctg atcgagggcg acctgaaccc cgacaacagc gacgtggaca agctgttcat
     1861 ccagctggtg cagacctaca accagctgtt cgaggaaaac cccatcaacg ccagcggcgt
     1921 ggacgccaag gccatcctgt ctgccagact gagcaagagc agacggctgg aaaatctgat
     1981 cgcccagctg cccggcgaga agaagaatgg cctgttcgga aacctgattg ccctgagcct
     2041 gggcctgacc cccaacttca agagcaactt cgacctggcc gaggatgcca aactgcagct
     2101 gagcaaggac acctacgacg acgacctgga caacctgctg gcccagatcg gcgaccagta
     2161 cgccgacctg tttctggccg ccaagaacct gtccgacgcc atcctgctga gcgacatcct
     2221 gagagtgaac accgagatca ccaaggcccc cctgagcgcc tctatgatca agagatacga
     2281 cgagcaccac caggacctga ccctgctgaa agctctcgtg cggcagcagc tgcctgagaa
     2341 gtacaaagag attttcttcg accagagcaa gaacggctac gccggctaca ttgacggcgg
     2401 agccagccag gaagagttct acaagttcat caagcccatc ctggaaaaga tggacggcac
     2461 cgaggaactg ctcgtgaagc tgaacagaga ggacctgctg cggaagcagc ggaccttcga
     2521 caacggcagc atcccccacc agatccacct gggagagctg cacgccattc tgcggcggca
     2581 ggaagatttt tacccattcc tgaaggacaa ccgggaaaag atcgagaaga tcctgacctt
     2641 ccgcatcccc tactacgtgg gccctctggc caggggaaac agcagattcg cctggatgac
     2701 cagaaagagc gaggaaacca tcaccccctg gaacttcgag gaagtggtgg acaagggcgc
     2761 ttccgcccag agcttcatcg agcggatgac caacttcgat aagaacctgc ccaacgagaa
     2821 ggtgctgccc aagcacagcc tgctgtacga gtacttcacc gtgtataacg agctgaccaa
     2881 agtgaaatac gtgaccgagg gaatgagaaa gcccgccttc ctgagcggcg agcagaaaaa
     2941 ggccatcgtg gacctgctgt tcaagaccaa ccggaaagtg accgtgaagc agctgaaaga
     3001 ggactacttc aagaaaatcg agtgcttcga ctccgtggaa atctccggcg tggaagatcg
     3061 gttcaacgcc tccctgggca cataccacga tctgctgaaa attatcaagg acaaggactt
     3121 cctggacaat gaggaaaacg aggacattct ggaagatatc gtgctgaccc tgacactgtt
     3181 tgaggacaga gagatgatcg aggaacggct gaaaacctat gcccacctgt tcgacgacaa
     3241 agtgatgaag cagctgaagc ggcggagata caccggctgg ggcaggctga gccggaagct
     3301 gatcaacggc atccgggaca agcagtccgg caagacaatc ctggatttcc tgaagtccga
     3361 cggcttcgcc aacagaaact tcatgcagct gatccacgac gacagcctga cctttaaaga
     3421 ggacatccag aaagcccagg tgtccggcca gggcgatagc ctgcacgagc acattgccaa
     3481 tctggccggc agccccgcca ttaagaaggg catcctgcag acagtgaagg tggtggacga
     3541 gctcgtgaaa gtgatgggcc ggcacaagcc cgagaacatc gtgatcgaaa tggccagaga
     3601 gaaccagacc acccagaagg gacagaagaa cagccgcgag agaatgaagc ggatcgaaga
     3661 gggcatcaaa gagctgggca gccagatcct gaaagaacac cccgtggaaa acacccagct
     3721 gcagaacgag aagctgtacc tgtactacct gcagaatggg cgggatatgt acgtggacca
     3781 ggaactggac atcaaccggc tgtccgacta cgatgtggac catatcgtgc ctcagagctt
     3841 tctgaaggac gactccatcg acaacaaggt gctgaccaga agcgacaaga accggggcaa
     3901 gagcgacaac gtgccctccg aagaggtcgt gaagaagatg aagaactact ggcggcagct
     3961 gctgaacgcc aagctgatta cccagagaaa gttcgacaat ctgaccaagg ccgagagagg
     4021 cggcctgagc gaactggata aggccggctt catcaagaga cagctggtgg aaacccggca
     4081 gatcacaaag cacgtggcac agatcctgga ctcccggatg aacactaagt acgacgagaa
     4141 tgacaagctg atccgggaag tgaaagtgat caccctgaag tccaagctgg tgtccgattt
     4201 ccggaaggat ttccagtttt acaaagtgcg cgagatcaac aactaccacc acgcccacga
     4261 cgcctacctg aacgccgtcg tgggaaccgc cctgatcaaa aagtacccta agctggaaag
     4321 cgagttcgtg tacggcgact acaaggtgta cgacgtgcgg aagatgatcg ccaagagcga
     4381 gcaggaaatc ggcaaggcta ccgccaagta cttcttctac agcaacatca tgaacttttt
     4441 caagaccgag attaccctgg ccaacggcga gatccggaag cggcctctga tcgagacaaa
     4501 cggcgaaacc ggggagatcg tgtgggataa gggccgggat tttgccaccg tgcggaaagt
     4561 gctgagcatg ccccaagtga atatcgtgaa aaagaccgag gtgcagacag gcggcttcag
     4621 caaagagtct atcctgccca agaggaacag cgataagctg atcgccagaa agaaggactg
     4681 ggaccctaag aagtacggcg gcttcgacag ccccaccgtg gcctattctg tgctggtggt
     4741 ggccaaagtg gaaaagggca agtccaagaa actgaagagt gtgaaagagc tgctggggat
     4801 caccatcatg gaaagaagca gcttcgagaa gaatcccatc gactttctgg aagccaaggg
     4861 ctacaaagaa gtgaaaaagg acctgatcat caagctgcct aagtactccc tgttcgagct
     4921 ggaaaacggc cggaagagaa tgctggcctc tgccggcgaa ctgcagaagg gaaacgaact
     4981 ggccctgccc tccaaatatg tgaacttcct gtacctggcc agccactatg agaagctgaa
     5041 gggctccagc ggcgggagcg gcgggagcgg ggggagcact aatctgagcg acatcattga
     5101 gaaggagact gggaaacagc tggtcattca ggagtccatc ctgatgctgc ctgaggaggt
     5161 ggaggaagtg atcggcaaca agccagagtc tgacatcctg gtgcacaccg cctacgacga
     5221 gtccacagat gagaatgtga tgctgctgac ctctgacgcc cccgagtata agccttgggc
     5281 cctggtcatc caggattcta acggcgagaa taagatcaag atgctgagcg gaggatccgg
     5341 aggatctgga ggcagcacca acctgtctga catcatcgag aaggagacag gcaagcagct
     5401 ggtcatccag gagagcatcc tgatgctgcc cgaagaagtc gaagaagtga tcggaaacaa
     5461 gcctgagagc gatatcctgg tccataccgc ctacgacgag agtaccgacg aaaatgtgat
     5521 gctgctgaca tccgacgccc cagagtataa gccctgggct ctggtcatcc aggattccaa
     5581 cggagagaac aaaatcaaaa tgctgtctgg cggctcaaaa agaaccgccg acggcagcga
     5641 attcgagccc aagaagaaga ggaaagtcta accggtcatc atcaccatca ccattgagtt
     5701 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     5761 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     5821 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     5881 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     5941 tctatggctt ctgaggcgga aagaaccagc tggggctcga taccgtcgac ctctagctag
     6001 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     6061 ccacacaaca tacgagccgg aagcataaag tgtaaagcct agggtgccta atgagtgagc
     6121 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     6181 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     6241 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     6301 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     6361 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     6421 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     6481 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     6541 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     6601 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     6661 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     6721 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     6781 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     6841 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     6901 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     6961 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     7021 atcttttcta cggggtctga cactcagtgg aacgaaaact cacgttaagg gattttggtc
     7081 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     7141 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     7201 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     7261 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     7321 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     7381 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     7441 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     7501 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     7561 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     7621 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     7681 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     7741 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     7801 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     7861 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     7921 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     7981 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     8041 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     8101 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     8161 gtgccacctg acgtcgacgg atcgggagat cgatctcccg atcccctagg gtcgactctc
     8221 agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg
     8281 gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac
     8341 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc
     8401 agatatacgc gttgacattg attattgact agttattaat agtaatcaat tacggggtca
     8461 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     8521 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     8581 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     8641 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     8701 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     8761 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
     8821 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     8881 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
     8941 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt
     9001 ttagtgaacc gtcagatccg ctag
//
