LOCUS       sequence-237976-        9287 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox-inducible FLAG/HA (N-terminal tag) SCAF8 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9287)
  AUTHORS   Gregersen LH, Mitter R, Ugalde AP, Nojima T, Proudfoot NJ, Agami R, 
            Stewart A, Svejstrup JQ
  TITLE     SCAF4 and SCAF8, mRNA Anti-Terminator Proteins.
  JOURNAL   Cell. 2019 May 10. pii: S0092-8674(19)30457-X. doi: 
            10.1016/j.cell.2019.04.038.
  PUBMED    31104839
REFERENCE   2  (bases 1 to 9287)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2019 
            May 10. pii: S0092-8674(19)30457-X. doi: 
            10.1016/j.cell.2019.04.038."
COMMENT     Sequence Label: pFRT/TO/FLAGHA-SCAF8
FEATURES             Location/Qualifiers
     source          1..9287
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    586..604
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    607..625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     635..659
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             759..782
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             783..809
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    825..849
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4807..4831)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4884..4925
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(5005..5022)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5011..5235
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5368..5387)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5519..5566
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5574..6596
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5574..6596
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6726..6847
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6763..6782)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6817..6836
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6896..6912)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6896..6912)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6909..6931)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6920..6936
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6944..6950,6951..6968,6969..6974))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6989..7010
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7127..7144)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7298..7886)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7378..7397)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8057..8848,8849..8917))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8680..8699
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8918..9022)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9097..9116)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata
      601 gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg
      661 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
      721 tccggactct agcgtttaaa cttaagcttg gtaccatgga ctacaaggac gacgatgaca
      781 agtaccctta tgacgtgccc gattacgctt tgaaccagat atcaacaagt ttgtacaaaa
      841 aagcaggctt caccatgccg cagccgctgc tgccagcgct tcctccttta agcagcccgt
      901 caggggctgg aagctgtgga ccatcaggag gggagtgcag tggccgccgc ctcttccgcc
      961 gccgggctcg gggcctccgc agcgacaaca tggaggccgt gaagaccttc aatagcgagt
     1021 tgtattccct gaatgactat aaaccaccca tttcgaaagc gaaaatgacc caaattacta
     1081 aggcagccat caaagctatt aagttctata aacatgtggt acagagtgtt gagaagttta
     1141 ttcagaaatg taaaccagaa tacaaagtac ctggacttta tgttattgac tccattgtgc
     1201 gacaatcccg acatcagttt ggtcaagaaa aggatgtgtt tgcacccaga tttagtaata
     1261 acatcattag cactttccag aatttatatc gttgccctgg ggatgacaag agtaaaatag
     1321 tgagagtact aaacttatgg cagaagaata atgtatttaa gagtgagatt attcagcccc
     1381 ttttggatat ggcagccggg attccgcctc cagttgtcac acctgttttg gccagcacta
     1441 ccactgctat gagcaatact ccaggaactc cagtcacgcc ggtcacgcct gccaatgtgg
     1501 ttcagggatt gccagaccca tgggtatctc agataacaaa tacagataca cttgcggctg
     1561 tagctcagat cttgcaaagt cctcaaggcc agcagcttca acaattaata caaaccttac
     1621 agatacaaca acagaagccc cagccttcca ttctgcaggc cctagatgct ggtcttgttg
     1681 ttcagttgca agctcttacg gcacaactta cagctgcagc tgcagctgcc aacactctta
     1741 ctcccttaga acagggagtc tcctttaaca agaagttgat ggataggttt gattttgggg
     1801 aagactctga gcatagtgaa gaacccaaaa aggaaattcc agcttcacaa ctttctcacg
     1861 tttcagaatc tgtgaacaat tccatttttc atcagatagc agaacaacta caacagcaaa
     1921 acctagaaca tctcagacag cagctcttgg agcagcaaca gcctcaaaag gccactcctc
     1981 aggatagtca ggaaggaacc tttgggtcag agcattcagc gtcaccatca caagggagta
     2041 gtcagcagca ttttcttgaa cctgaagtca atttggatga ttccatagat attcagcaac
     2101 aggatatgga tatagatgaa gggcaagatg gagtggaaga ggaggtcttt gaacaagaag
     2161 ctaagaaagt ggcggttcgc tcaagatcaa gaacacattc acgatctcgt tcaagatcac
     2221 caagaaaacg aaggtctagg tcacggtctg gctctagaaa gcgtaaacac agaaagcgat
     2281 cacgctcccg ctcaagagaa agaaagagga aatcatcacg gtcgtattca agtgaaagga
     2341 gagccagaga aagggagaaa gaacgacaga aaaagggatt acctccaatt agatctaaaa
     2401 cactaagtgt atgtagtact actctctggg ttgggcaagt ggacaagaag gcaacacagc
     2461 aagacttaac caacctgttt gaagagtttg gacagattga atccattaat atgattcctc
     2521 cgcgaggctg tgcttatgtc tgcatggttc atcgacaaga tgcatttcga gctcttcaga
     2581 aactcagttc tggatcatat aaaattgggt ccaaggtcat taagatcgct tgggctttaa
     2641 acaaaggtgt aaaaacagaa tacaaacaat tctgggatgt ggatcttgga gttacatata
     2701 taccatggga aaaagttaaa gtggatgact tggaaggttt tgcagaagga ggcatgattg
     2761 atcaggagac tgtaaatact gagtgggaaa ctgtgaaaag ctcagaacct gttaaagaga
     2821 cggtccagac aactcagagc ccaactccag ttgaaaagga gacagtggtc acaacccagg
     2881 cagaggtttt ccctcctcct gttgctatgt tgcagattcc agtggcgcca gccgtgccta
     2941 cagttagttt agtcccacca gcatttcctg tgtcgatgcc ggttcctcct cctggattca
     3001 gtccaatccc tccacctcct tttttaagag caagttttaa cccttcacaa ccaccacctg
     3061 gtttcatgcc gcctccagtt cccccacctg ttgtgccacc ccctacgatt ccaccagtag
     3121 taccaacatc tttagtgcag ccgtcattat ccatgacacc ggaaactgtg aaagatgttg
     3181 gatttggtag ccttgttata ccaggcggtt ctgttgccag caatcttgct acttccgctc
     3241 tgccagctgg aaatgttttt aatgctccaa ctaaacaggc agagcctgaa gaaaaagtac
     3301 ctcatcttat agaccaccag atttcttctg gtgaaaacac cagatcagtg attccaaatg
     3361 atatttcaag taatgctgca attttaggag gacagccgcc aaatgtgaca agcaattctg
     3421 gaattctggg agtccaaaga ccaaatgtat caagtaattc tgaaattctt ggggtccggc
     3481 catctaatgt ttccagtagt tctgggatta ttgcagccca accaccaaat attctaaata
     3541 actctggaat attgggaata cagccaccca gtgtgtcaaa tagttctgga cttttgggag
     3601 tgctaccccc aaatatacct aacaattctg gacttgtagg agtacagcca ccaaatgttc
     3661 caaatactcc tggacttctg ggaacacagc caccagctgg acctcaaaac ttaccccctt
     3721 taagtatccc taatcaaagg atgcccacaa tgccaatgtt agacattcgt ccgggactaa
     3781 taccacaggc acctgggcca agattccctt taatacagcc tggaattcca ccccaacggg
     3841 gaatcccacc cccatcggta cttgattcag ctcttcatcc accaccccgt ggaccttttc
     3901 ctccaggaga tatttttagt caaccagaaa gacctttttt agctcctgga agacaaagcg
     3961 tagacaatgt tactaaccca gaaaaaagga taccacttgg gaatgataac attcaacagg
     4021 aaggagatag agattatcgg tttcctccta tagaaaccag ggaaagcatt agtagacctc
     4081 cccctgtgga tgttagagat gtggttgggc ggcctataga tccaagagaa ggtcctggac
     4141 ggcctccact agatggtagg gatcattttg gaagacctcc tgtagatata agagagaatc
     4201 ttgtgaggcc aggtatagat catcttggtc gaagagacca ctttggcttt aatccagaga
     4261 agccctgggg gcatagagga gattttgatg agagagagca tcgggttcta ccggtctatg
     4321 gtggtccaaa aggcttacat gaagaaagag gtagatttcg gtctggaaac tatcgatttg
     4381 atcctagaag tggtccttgg aaccgaggat ttggacaaga agttcacaga gattttgatg
     4441 accgcagaag accctgggag aggcaaaggg atagggatga cagagatttt gatttctgca
     4501 gagaaatgaa tggaaatcgt cttggacgag acagaattca aaacacttgg gttccccctc
     4561 ctcatgctcg ggtttttgat tattttgaag gggccacttc tcaacgaaaa ggtgataatg
     4621 tgcctcaggt taatggtgaa aatacagaga gacatgctca gccaccacct ataccagtac
     4681 agaatgatcc tgaactttat gaaaaactga catcttcaaa tgaaataaac aaggagaaga
     4741 gtgacacagt tgctgatata gaaagtgaac cagtggtaga aagcacagaa actgagggga
     4801 cataaaaccc agctttcttg tacaaagtgg ttgatatcca gcacagtggc ggccgctcaa
     4861 gtctagaggg cccgcggttc gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt
     4921 ctacgcgtac ctgatccgcg gccgcataga taactgatcc agtgtgcctc gagtctagag
     4981 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     5041 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     5101 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     5161 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     5221 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     5281 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     5341 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     5401 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     5461 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtacctaga
     5521 agttcctatt ccgaagttcc tattctctag aaagtatagg aacttccttg gccaaaaagc
     5581 ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg
     5641 acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc
     5701 gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt
     5761 atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattca
     5821 gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc
     5881 ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg
     5941 cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat
     6001 acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa
     6061 ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt
     6121 gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg
     6181 tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg
     6241 attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc
     6301 agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg
     6361 cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg
     6421 atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg
     6481 tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag
     6541 tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaatagcacg
     6601 tactacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     6661 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     6721 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     6781 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     6841 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     6901 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     6961 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     7021 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     7081 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     7141 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     7201 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     7261 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     7321 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     7381 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     7441 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     7501 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     7561 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     7621 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     7681 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     7741 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     7801 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     7861 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     7921 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     7981 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     8041 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     8101 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     8161 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     8221 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     8281 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     8341 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     8401 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     8461 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     8521 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     8581 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     8641 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     8701 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     8761 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     8821 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     8881 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     8941 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     9001 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
     9061 cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata
     9121 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa
     9181 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt
     9241 taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgtt
//
