LOCUS       sequence-237978-        6626 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Shuttle vector for use in AdEasy System. Used for expression of 
            transgenes under a chosen (non-CMV) promoter when no GFP tracer is 
            desired..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6626)
  AUTHORS   He TC, Zhou S, da Costa LT, Yu J, Kinzler KW, Vogelstein B
  TITLE     A simplified system for generating recombinant adenoviruses.
  JOURNAL   Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14.
  PUBMED    9482916
REFERENCE   2  (bases 1 to 6626)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1998 Mar 3. 95(5):2509-14."
COMMENT     Sequence Label: pShuttle
FEATURES             Location/Qualifiers
     source          1..6626
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..103
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     misc_signal     191..341
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     repeat_region   3585..3687
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     primer_bind     complement(3776..3793)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3947..4535)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4027..4046)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             5367..6161
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5367..6161
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5421..5440)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6031..6050
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(6209..6589)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6271..6292)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6483..6502
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt
       61 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt
      121 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg
      181 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag
      241 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga
      301 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tactggtacc gcggccgcct
      361 cgagtctaga gatatcgaat tcaagcttgt cgactcgaag atctgggcgt ggttaagggt
      421 gggaaagaat atataaggtg ggggtcttat gtagttttgt atctgttttg cagcagccgc
      481 cgccgccatg agcaccaact cgtttgatgg aagcattgtg agctcatatt tgacaacgcg
      541 catgccccca tgggccgggg tgcgtcagaa tgtgatgggc tccagcattg atggtcgccc
      601 cgtcctgccc gcaaactcta ctaccttgac ctacgagacc gtgtctggaa cgccgttgga
      661 gactgcagcc tccgccgccg cttcagccgc tgcagccacc gcccgcggga ttgtgactga
      721 ctttgctttc ctgagcccgc ttgcaagcag tgcagcttcc cgttcatccg cccgcgatga
      781 caagttgacg gctcttttgg cacaattgga ttctttgacc cgggaactta atgtcgtttc
      841 tcagcagctg ttggatctgc gccagcaggt ttctgccctg aaggcttcct cccctcccaa
      901 tgcggtttaa aacataaata aaaaaccaga ctctgtttgg atttggatca agcaagtgtc
      961 ttgctgtctt tatttagggg ttttgcgcgc gcggtaggcc cgggaccagc ggtctcggtc
     1021 gttgagggtc ctgtgtattt tttccaggac gtggtaaagg tgactctgga tgttcagata
     1081 catgggcata agcccgtctc tggggtggag gtagcaccac tgcagagctt catgctgcgg
     1141 ggtggtgttg tagatgatcc agtcgtagca ggagcgctgg gcgtggtgcc taaaaatgtc
     1201 tttcagtagc aagctgattg ccaggggcag gcccttggtg taagtgttta caaagcggtt
     1261 aagctgggat gggtgcatac gtggggatat gagatgcatc ttggactgta tttttaggtt
     1321 ggctatgttc ccagccatat ccctccgggg attcatgttg tgcagaacca ccagcacagt
     1381 gtatccggtg cacttgggaa atttgtcatg tagcttagaa ggaaatgcgt ggaagaactt
     1441 ggagacgccc ttgtgacctc caagattttc catgcattcg tccataatga tggcaatggg
     1501 cccacgggcg gcggcctggg cgaagatatt tctgggatca ctaacgtcat agttgtgttc
     1561 caggatgaga tcgtcatagg ccatttttac aaagcgcggg cggagggtgc cagactgcgg
     1621 tataatggtt ccatccggcc caggggcgta gttaccctca cagatttgca tttcccacgc
     1681 tttgagttca gatgggggga tcatgtctac ctgcggggcg atgaagaaaa cggtttccgg
     1741 ggtaggggag atcagctggg aagaaagcag gttcctgagc agctgcgact taccgcagcc
     1801 ggtgggcccg taaatcacac ctattaccgg ctgcaactgg tagttaagag agctgcagct
     1861 gccgtcatcc ctgagcaggg gggccacttc gttaagcatg tccctgactc gcatgttttc
     1921 cctgaccaaa tccgccagaa ggcgctcgcc gcccagcgat agcagttctt gcaaggaagc
     1981 aaagtttttc aacggtttga gaccgtccgc cgtaggcatg cttttgagcg tttgaccaag
     2041 cagttccagg cggtcccaca gctcggtcac ctgctctacg gcatctcgat ccagcatatc
     2101 tcctcgtttc gcgggttggg gcggctttcg ctgtacggca gtagtcggtg ctcgtccaga
     2161 cgggccaggg tcatgtcttt ccacgggcgc agggtcctcg tcagcgtagt ctgggtcacg
     2221 gtgaaggggt gcgctccggg ctgcgcgctg gccagggtgc gcttgaggct ggtcctgctg
     2281 gtgctgaagc gctgccggtc ttcgccctgc gcgtcggcca ggtagcattt gaccatggtg
     2341 tcatagtcca gcccctccgc ggcgtggccc ttggcgcgca gcttgccctt ggaggaggcg
     2401 ccgcacgagg ggcagtgcag acttttgagg gcgtagagct tgggcgcgag aaataccgat
     2461 tccggggagt aggcatccgc gccgcaggcc ccgcagacgg tctcgcattc cacgagccag
     2521 gtgagctctg gccgttcggg gtcaaaaacc aggtttcccc catgcttttt gatgcgtttc
     2581 ttacctctgg tttccatgag ccggtgtcca cgctcggtga cgaaaaggct gtccgtgtcc
     2641 ccgtatacag acttgagagg gagtttaaac gaattcaata gcttgttgca tgggcggcga
     2701 tataaaatgc aaggtgctgc tcaaaaaatc aggcaaagcc tcgcgcaaaa aagaaagcac
     2761 atcgtagtca tgctcatgca gataaaggca ggtaagctcc ggaaccacca cagaaaaaga
     2821 caccattttt ctctcaaaca tgtctgcggg tttctgcata aacacaaaat aaaataacaa
     2881 aaaaacattt aaacattaga agcctgtctt acaacaggaa aaacaaccct tataagcata
     2941 agacggacta cggccatgcc ggcgtgaccg taaaaaaact ggtcaccgtg attaaaaagc
     3001 accaccgaca gctcctcggt catgtccgga gtcataatgt aagactcggt aaacacatca
     3061 ggttgattca catcggtcag tgctaaaaag cgaccgaaat agcccggggg aatacatacc
     3121 cgcaggcgta gagacaacat tacagccccc ataggaggta taacaaaatt aataggagag
     3181 aaaaacacat aaacacctga aaaaccctcc tgcctaggca aaatagcacc ctcccgctcc
     3241 agaacaacat acagcgcttc cacagcggca gccataacag tcagccttac cagtaaaaaa
     3301 gaaaacctat taaaaaaaca ccactcgaca cggcaccagc tcaatcagtc acagtgtaaa
     3361 aaagggccaa gtgcagagcg agtatatata ggactaaaaa atgacgtaac ggttaaagtc
     3421 cacaaaaaac acccagaaaa ccgcacgcga acctacgccc agaaacgaaa gccaaaaaac
     3481 ccacaacttc ctcaaatcgt cacttccgtt ttcccacgtt acgtcacttc ccattttaag
     3541 aaaactacaa ttcccaacac atacaagtta ctccgcccta aaacctacgt cacccgcccc
     3601 gttcccacgc cccgcgccac gtcacaaact ccaccccctc attatcatat tggcttcaat
     3661 ccaaaataag gtatattatt gatgatgtta attaacatgc atggatccat atgcggtgtg
     3721 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc
     3781 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     3841 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     3901 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     3961 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     4021 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     4081 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     4141 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     4201 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     4261 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     4321 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     4381 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     4441 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     4501 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     4561 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     4621 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     4681 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     4741 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     4801 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     4861 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     4921 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     4981 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcagccatg agattatcaa
     5041 aaaggatctt cacctagatc cttttcacgt agaaagccag tccgcagaaa cggtgctgac
     5101 cccggatgaa tgtcagctac tgggctatct ggacaaggga aaacgcaagc gcaaagagaa
     5161 agcaggtagc ttgcagtggg cttacatggc gatagctaga ctgggcggtt ttatggacag
     5221 caagcgaacc ggaattgcca gctggggcgc cctctggtaa ggttgggaag ccctgcaaag
     5281 taaactggat ggctttcttg ccgccaagga tctgatggcg caggggatca agctctgatc
     5341 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
     5401 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     5461 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     5521 acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca
     5581 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     5641 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     5701 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     5761 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     5821 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     5881 ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     5941 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     6001 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     6061 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     6121 agcgcatcgc cttctatcgc cttcttgacg agttcttctg aattttgtta aaatttttgt
     6181 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     6241 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     6301 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     6361 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     6421 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     6481 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     6541 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc cattcgccat
     6601 tcaggatcga attaattctt aattaa
//
