LOCUS       sequence-237980-        1365 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Chimeric ETS domain: H3/S3 of Ets-1 in PU.1 scaffold.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Albrecht AV, Kim HM, Poon GMK
  TITLE     Mapping interfacial hydration in ETS-family transcription factor 
            complexes with DNA: a chimeric approach.
  JOURNAL   Nucleic Acids Res. 2018 Nov 16;46(20):10577-10588. doi: 
            10.1093/nar/gky894.
  PUBMED    30295801
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2018-11-16- 16"; volume: "46"; issue: 
            "20"; pages: "10577-10588"
COMMENT     Sequence Label: pET28B-PU.1-ETS-H3/S3
FEATURES             Location/Qualifiers
     source          1..1365
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             11..33
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             361..378
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             385..402
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             427..444
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(497..515)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      511..558
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      595..1050
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(682..701)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     892..913
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ctannntaat tttgtttaac tttaagaagg agatatacca tggggtcaaa gaaaaaaatc
       61 cgcctttatc aatttctgtt ggacttgttg cgtagcggcg acatgaagga cagcatctgg
      121 tgggttgaca aggacaaggg tacattccaa tttagcagta agcacaaaga agccctggca
      181 catcgctggg gcatccagaa gggtaaccgt aagaagatga cgtatcaaaa aatggcccgc
      241 gctcttcgta attattacga caaaaatgag gtcaaaaagg tgaaaaaaaa gttgacatat
      301 caattcagtg gagaagtact gggccgtggc gggcttgcgg agcgtcgttt gccaccccat
      361 cttgttccac gcggtagccg ttctcatcac caccaccacc attaaaagct tgcggccgca
      421 ctcgagcacc accaccacca ccactgagat ccggctgcta acaaagcccg aaaggaagct
      481 gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg
      541 gtcttgaggg gttttttgct gaaaggagga actatatccg gattggcgaa tgggacgcgc
      601 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
      661 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
      721 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
      781 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
      841 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
      901 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
      961 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     1021 attttaacaa aatattaacg cttacaattt aggtggcact tttcgggnaa atgtgcgcgg
     1081 aacccctatt tgtttatttt tctaaanaca ttcaaatatg tatccgccnn ngaattaatt
     1141 cttnnaaaan nncatcnnng catcaantga annnnnnatt tnttccttnc cggntnnnna
     1201 nnccnaattt tnnaaaaanc nnttnnnnnn atnnannnaa annnnncccc nnnncnnttc
     1261 nnnaagaagg nangaacnng nnannggntn ncnnttccan ctnnccaann nnnnnnncnn
     1321 nnannttncc ccccccaaaa aanggttnnn nngnaaannc ccnng
//
