LOCUS       sequence-237990-        4618 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of inositol 1,4,5-trisphosphate 3-kinase A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4618)
  AUTHORS   Takazawa K, Vandekerckhove J, Dumont JE, Erneux C
  TITLE     Cloning and expression in Escherichia coli of a rat brain cDNA 
            encoding a Ca2+/calmodulin-sensitive inositol 1,4,5-trisphosphate 
            3-kinase.
  JOURNAL   Biochem J. 1990 Nov 15;272(1):107-12.
  PUBMED    2176078
REFERENCE   2  (bases 1 to 4618)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem 
            J."; date: "1990-11-15"; volume: "272(1)"; pages: "107-12"
COMMENT     Sequence Label: rat brain inositol 1,4,5-trisphosphate 3-kinas...
FEATURES             Location/Qualifiers
     source          1..4618
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      7..462
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(94..113)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     304..325
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     589..611
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     603..620
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     604..620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     627..646
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        627..645
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     670..686
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     671..687
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2380..2396)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2380..2396)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(2433..2451)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2472..2488)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2472..2488)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2485..2507)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2496..2512
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2520..2526,2527..2544,2545..2550))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2565..2586
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2703..2720)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2874..3462)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2954..2973)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3633..4424,4425..4493))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4256..4275
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4494..4598)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
       61 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
      121 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
      181 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
      241 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
      301 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
      361 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
      421 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttgccattcg ccattcaggc
      481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      541 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      601 gttgtaaaac gacggccagt gaattgtaat acgactcact atagggcgaa ttgggtaccg
      661 ggccccccct cgaggtcgac ggtatcgata agcttgatat cgaattcctt tttttttttt
      721 tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt
      781 tttttttgaa tctttgagtt ttattaggaa gggtgtccat ggtagtgtag gaagttagac
      841 cagtaagtct atatacctgt gaagtacaat tctggatcgg gaccctctgg agaggtgcaa
      901 agtcacttga caaggctggg ggagaagccc actgtttcct tcctctccta taaattagtg
      961 tcattcagct ctgggagaca ggcttgtgta gaagccagct cctaatgtac tacaccccct
     1021 tagtggatgg cacctggtcc tccaggacca tgtgaactgc ccgcccagcg gggttccagt
     1081 ctcttgccgg catgcatggt ggggatgcag ccacaggtcc acatctctga ccagctggcc
     1141 caagctgagg gaaaagggcc cagcctcatc tctcagccag gttggccaag atgccaatga
     1201 gattgtccag ccccagcaaa tagccgtcct cacgattgcc ctcctcccag ggcctccgat
     1261 gatccaggat ctggccatcg gggagaggcg tggtcttgcc aaaatcgatg agccacacac
     1321 cagcgcgatg gcaatggtca tgcacgaaga ggagtgagct gccaatcacc tcgtgccgtc
     1381 taaagaaatc agagatctcc agggtatccc ggatctgctg taggcggttc agatacctct
     1441 tcagcacttc agcatctcct tgcatgaact cctcaaagac tcgagtcact tgctctctgc
     1501 ttcgtgtagt tttgaaatca gtactgcaag atccatcggc tttcttgatg ccctcgatgc
     1561 ggaagccgag tgtagtgctg gagctgatgc cttcgcgcca ttgcatgtat cgaggttttg
     1621 tgacggcacg ctgcgcatgc tcctcctcag taggtgcctc ggggtccacg gccagcattt
     1681 tcttatacat gtcctttcgc agcttgggcc gttctcgggc tttggtcagc tcttcttcca
     1741 ggtaagttct gacacccatc ttgcagtcaa gcacgcaagg tccatcgaag ccatcaagca
     1801 ggtcctgtaa ctgtaagtag ctttcaccat cccgctcgac cacaccgtgg aaggccggca
     1861 cacacccacg cagcacatca gccatcaacc gcaccaggca gtagtgttca ggctccgagc
     1921 tgcgcttcag gatcaggccg ctggtgcccg cagctttgaa actccctgtg tgccctgcta
     1981 actgcaccca ggagtagcgc tttttgaaag gactcatgac aggcaggttg accatggtac
     2041 ggatcttctg ccaatggctt ttctgcccca cgtcttcgct ggtttccagc tgcacgttgc
     2101 cgcggctccg gctctcgctg tcgctcagca ggtcgtcctc cgagtcctcg agcagcgacg
     2161 aggagccggt ggaggagagg gacgaggtgg agaggcggcg tggctgctgc aggtgcgacc
     2221 ccgcagctgg gagccagttc ccctcccggt ccggcggccc ggggctggcc ggggccacat
     2281 cctcctcgct tgtcacagtg agctgcggga tcaccggagc aggggcagca cgcgggagcc
     2341 cgttaggcac cagttccccg gaattcctgc agcccggggg atccactagt tctagagcgg
     2401 ccgccaccgc ggtggagctc cagcttttgt tccctttagt gagggttaat ttcgagcttg
     2461 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     2521 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     2581 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     2641 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     2701 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     2761 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     2821 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     2881 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     2941 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     3001 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     3061 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     3121 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     3181 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     3241 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     3301 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     3361 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     3421 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     3481 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     3541 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     3601 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     3661 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     3721 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     3781 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     3841 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     3901 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     3961 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     4021 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     4081 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     4141 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     4201 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     4261 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     4321 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     4381 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     4441 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     4501 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     4561 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgc
//
