LOCUS       sequence-237993-         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_br58_g4698
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(438..454)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(438..454)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(451..473)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    462..478
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(486..492,493..510,511..516))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    531..552
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(669..686)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttggtctcta atgtctgatt tttggaaagt tgatttgtat ggtccaccac aaggtcattc
       61 taatgttaaa tctgaaattg ttgctactgt tgtttgtggt gatggtagaa aagctaattt
      121 gtcttatact cattcttgga gaggtgattc tactactaaa actattacta ttagatgtag
      181 aattactcca aaagttatta aaggtggtcc attgccacca tattatgaat tgaaagctga
      241 accaatttgg gctggtgatg aaagagctat tgctgctgct aaaagagaac aagaagaaag
      301 agttttggaa caatttgaat tgatgaattt gatgatggat gcttctgaag atccaaaagc
      361 tagaagattg tttgcttctc catgagctta gagaccaagt tccatcccaa tggcgcgccg
      421 agcttggctc gagcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
      481 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
      541 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
      601 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
      661 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
      721 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
//
