LOCUS       sequence-237994-        6747 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pDONR for Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6747)
  AUTHORS   Gregersen LH, Mitter R, Ugalde AP, Nojima T, Proudfoot NJ, Agami R, 
            Stewart A, Svejstrup JQ
  TITLE     SCAF4 and SCAF8, mRNA Anti-Terminator Proteins.
  JOURNAL   Cell. 2019 May 10. pii: S0092-8674(19)30457-X. doi: 
            10.1016/j.cell.2019.04.038.
  PUBMED    31104839
REFERENCE   2  (bases 1 to 6747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2019 
            May 10. pii: S0092-8674(19)30457-X. doi: 
            10.1016/j.cell.2019.04.038."
COMMENT     Sequence Label: pDONR223 SCAF8_isoC_CRISPR resistant
FEATURES             Location/Qualifiers
     source          1..6747
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(4040..4139)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4156..4175)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4157..4175)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4180..4196)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4180..4196)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4627..5418
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5511..6099
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6000..6019
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6253..6270
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(6429..6456)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(6548..6634)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     6683..6705
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6697..6714
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6698..6714
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    join(6730..6747,1..82)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca
       61 actttgtaca aaaaagcagg cttcaccatg ccgcagccgc tgctgccagc gcttcctcct
      121 ttaagcagcc cgtcaggggc tggaagctgt ggaccatcag gaggggagtg cagtggccgc
      181 cgcctcttcc gccgccgggc tcggggcctc cgcagcgaca acatggaggc cgtgaagacc
      241 ttcaatagcg agttgtattc cctgaatgac tataaaccac ccatttcgaa agcgaaaatg
      301 acccaaatta ctaaggcagc catcaaagct attaagttct ataaacatgt ggtacagagt
      361 gttgagaagt ttattcagaa atgtaaacca gaatacaaag tacctggact ttatgttatt
      421 gactccattg tgcgacaatc ccgacatcag tttggtcaag aaaaggatgt gtttgcaccc
      481 agatttagta ataacatcat tagcactttc cagaatttat atcgttgccc tggggatgac
      541 aagagtaaaa tagtgagagt actaaactta tggcagaaga ataatgtatt taagagtgag
      601 attattcagc cccttttgga tatggcagcc gggattccgc ctccagttgt cacacctgtt
      661 ttggccagca ctaccactgc tatgagcaat actccaggaa ctccagtcac gccggtcacg
      721 cctgccaatg tggttcaggg attgccagac ccatgggtat ctcagataac aaatacagat
      781 acacttgcgg ctgtagctca gatcttgcaa agtcctcaag gccagcagct tcaacaatta
      841 atacaaacct tacagataca acaacagaag ccccagcctt ccattctgca ggccctagat
      901 gctggtcttg ttgttcagtt gcaagctctt acggcacaac ttacagctgc agctgcagct
      961 gccaacactc ttactccctt agaacaggga gtctccttta acaagaagtt gatggatagg
     1021 tttgattttg gggaagactc tgagcatagt gaagaaccca aaaaggaaat tccagcttca
     1081 caactttctc acgtttcaga atctgtgaac aattccattt ttcatcagat agcagaacaa
     1141 ctacaacagc aaaacctaga acatctcaga cagcagctct tggagcagca acagcctcaa
     1201 aaggccactc ctcaggatag tcaggaagga acctttgggt cagagcattc agcgtcacca
     1261 tcacaaggga gtagtcagca gcattttctt gaacctgaag tcaatttgga tgattccata
     1321 gatattcagc aacaggatat ggatatagat gaagggcaag atggagtgga agaggaggtc
     1381 tttgaacaag aagctaagaa agtggcggtt cgctcaagat caagaacaca ttcacgatct
     1441 cgttcaagat caccaagaaa acgaaggtct aggtcacggt ctggctctag aaagcgtaaa
     1501 cacagaaagc gatcacgctc ccgctcaaga gaaagaaaga ggaaatcatc acggtcgtat
     1561 tcaagtgaaa ggagagccag agaaagggag aaagaacgac agaaaaaggg attacctcca
     1621 attagatcta aaacactaag tgtatgtagt actactctct gggttgggca agtggacaag
     1681 aaggcaacac agcaagactt aaccaacctg tttgaagagt ttggacagat tgaatccatt
     1741 aatatgattc ctccgcgagg ctgtgcttat gtctgcatgg ttcatcgaca agatgcattt
     1801 cgagctcttc agaaactcag ttctggatca tataaaattg ggtccaaggt cattaagatc
     1861 gcttgggctt taaacaaagg tgtaaaaaca gaatacaaac aattctggga tgtggatctt
     1921 ggagttacat atataccatg ggaaaaagtt aaagtggatg acttggaagg ttttgcagaa
     1981 ggaggcatga ttgatcagga gactgtaaat actgagtggg aaactgtgaa aagctcagaa
     2041 cctgttaaag agacggtcca gacaactcag agcccaactc cagttgaaaa ggagacagtg
     2101 gtcacaaccc aggcagaggt tttccctcct cctgttgcta tgttgcagat tccagtggcg
     2161 ccagccgtgc ctacagttag tttagtccca ccagcatttc ctgtgtcgat gccggttcct
     2221 cctcctggat tcagtccaat ccctccacct ccttttttaa gagcaagttt taacccttca
     2281 caaccaccac ctggtttcat gccgcctcca gttcccccac ctgttgtgcc accccctacg
     2341 attccaccag tagtaccaac atctttagtg cagccgtcat tatccatgac accggaaact
     2401 gtgaaagatg ttggatttgg tagccttgtt ataccaggcg gttctgttgc cagcaatctt
     2461 gctacttccg ctctgccagc tggaaatgtt tttaatgctc caactaaaca ggcagagcct
     2521 gaagaaaaag tacctcatct tatagaccac cagatttctt ctggtgaaaa caccagatca
     2581 gtgattccaa atgatatttc aagtaatgct gcaattttag gaggacagcc gccaaatgtg
     2641 acaagcaatt ctggaattct gggagtccaa agaccaaatg tatcaagtaa ttctgaaatt
     2701 cttggggtcc ggccatctaa tgtttccagt agttctggga ttattgcagc ccaaccacca
     2761 aatattctaa ataactctgg aatattggga atacagccac ccagtgtgtc aaatagttct
     2821 ggacttttgg gagtgctacc cccaaatata cctaacaatt ctggacttgt aggagtacag
     2881 ccaccaaatg ttccaaatac tcctggactt ctgggaacac agccaccagc tggacctcaa
     2941 aacttacccc ctttaagtat ccctaatcaa aggatgccca caatgccaat gttagacatt
     3001 cgtccgggac taataccaca ggcacctggg ccaagattcc ctttaataca gcctggaatt
     3061 ccaccccaac ggggaatccc acccccatcg gtacttgatt cagctcttca tccaccaccc
     3121 cgtggacctt ttcctccagg agatattttt agtcaaccag aaagaccttt tttagctcct
     3181 ggaagacaaa gcgtagacaa tgttactaac ccagaaaaaa ggataccact tgggaatgat
     3241 aacattcaac aggaaggaga tagagattat cggtttcctc ctatagaaac cagggaaagc
     3301 attagtagac ctccccctgt ggatgttaga gatgtggttg ggcggcctat agatccaaga
     3361 gaaggtcctg gacggcctcc actagatggt agggatcatt ttggaagacc tcctgtagat
     3421 ataagagaga atcttgtgag gccaggtata gatcatcttg gtcgaagaga ccactttggc
     3481 tttaatccag agaagccctg ggggcataga ggagattttg atgagagaga gcatcgggtt
     3541 ctaccggtct atggtggtcc aaaaggctta catgaagaaa gaggtagatt tcggtctgga
     3601 aactatcgat ttgatcctag aagtggtcct tggaaccgag gatttggaca agaagttcac
     3661 agagattttg atgaccgcag aagaccctgg gagaggcaaa gggataggga tgacagagat
     3721 tttgatttct gcagagaaat gaatggaaat cgtcttggac gagacagaat tcaaaacact
     3781 tgggttcccc ctcctcatgc tcgggttttt gattattttg aaggggccac ttctcaacga
     3841 aaaggtgata atgtgcctca ggttaatggt gaaaatacag agagacatgc tcagccacca
     3901 cctataccag tacagaatga tcctgaactt tatgaaaaac tgacatcttc aaatgaaata
     3961 aacaaggaga agagtgacac agttgctgat atagaaagtg aaccagtggt agaaagcaca
     4021 gaaactgagg ggacataaaa cccagctttc ttgtacaaag ttggcattat aagaaagcat
     4081 tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc
     4141 catccagctg atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag
     4201 ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca
     4261 tgcctcctct agaccagcca ggacagaaat gcctcgactt cgctgctacc caaggttgcc
     4321 gggtgacgca caccgtggaa acggatgaag gcacgaaccc agtggacata agcctgttcg
     4381 gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc
     4441 gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt
     4501 ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt
     4561 ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta
     4621 aacattatga gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc
     4681 gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg
     4741 gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt
     4801 gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga
     4861 gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg
     4921 tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt
     4981 gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca
     5041 agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct
     5101 gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac
     5161 tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta
     5221 accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc
     5281 cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc
     5341 ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc
     5401 aaggtagtcg gcaaataacc ctcgagccac ccatgaccaa aatcccttaa cgtgagttac
     5461 gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     5521 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     5581 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     5641 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     5701 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     5761 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     5821 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     5881 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     5941 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     6001 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     6061 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     6121 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     6181 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     6241 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     6301 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     6361 aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta
     6421 gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt
     6481 atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc
     6541 ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg
     6601 cccagtcttc cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgcg
     6661 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaag
     6721 ctcgggcccc aaataatgat tttattt
//
