LOCUS       sequence-237997-        6612 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pDONR for Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6612)
  AUTHORS   Gregersen LH, Mitter R, Ugalde AP, Nojima T, Proudfoot NJ, Agami R, 
            Stewart A, Svejstrup JQ
  TITLE     SCAF4 and SCAF8, mRNA Anti-Terminator Proteins.
  JOURNAL   Cell. 2019 May 10. pii: S0092-8674(19)30457-X. doi: 
            10.1016/j.cell.2019.04.038.
  PUBMED    31104839
REFERENCE   2  (bases 1 to 6612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2019 
            May 10. pii: S0092-8674(19)30457-X. doi: 
            10.1016/j.cell.2019.04.038."
COMMENT     Sequence Label: pDONR223 SCAF8_isoD_CRISPR_resistant
FEATURES             Location/Qualifiers
     source          1..6612
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1..87)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     136..158
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     150..167
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     151..167
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    183..282
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4105..4204)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4221..4240)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4222..4240)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4245..4261)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4245..4261)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4692..5483
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5576..6164
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6065..6084
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6318..6335
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(6494..6521)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc
       61 ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcgttaacgc
      121 tagcatggat gttttcccag tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc
      181 cccaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc
      241 aatgcttttt tataatgcca actttgtaca aaaaagcagg cttcaccatg gaggccgtga
      301 agaccttcaa tagcgagttg tattccctga atgactataa accacccatt tcgaaagcga
      361 aaatgaccca aattactaag gcagccatca aagctattaa gttctataaa catgtggtac
      421 agagtgttga gaagtttatt cagaaatgta aaccagaata caaagtacct ggactttatg
      481 ttattgactc cattgtgcga caatcccgac atcagtttgg tcaagaaaag gatgtgtttg
      541 cacccagatt tagtaataac atcattagca ctttccagaa tttatatcgt tgccctgggg
      601 atgacaagag taaaatagtg agagtactaa acttatggca gaagaataat gtatttaaga
      661 gtgagattat tcagcccctt ttggatatgg cagccgggat tccgcctcca gttgtcacac
      721 ctgttttggc cagcactacc actgctatga gcaatactcc aggaactcca gtcacgccgg
      781 tcacgcctgc caatgtggtt cagggattgc cagacccatg ggtatctcag ataacaaata
      841 cagatacact tgcggctgta gctcagatct tgcaaagtcc tcaaggccag cagcttcaac
      901 aattaataca aaccttacag atacaacaac agaagcccca gccttccatt ctgcaggccc
      961 tagatgctgg tcttgttgtt cagttgcaag ctcttacggc acaacttaca gctgcagctg
     1021 cagctgccaa cactcttact cccttagaac agggagtctc ctttaacaag aagttgatgg
     1081 ataggtttga ttttggggaa gactctgagc atagtgaaga acccaaaaag gaaattccag
     1141 cttcacaact ttctcacgtt tcagaatctg tgaacaattc catttttcat cagatagcag
     1201 aacaactaca acagcaaaac ctagaacatc tcagacagca gctcttggag cagcaacagc
     1261 ctcaaaaggc cactcctcag gatagtcagg aaggaacctt tgggtcagag cattcagcgt
     1321 caccatcaca agggagtagt cagcagcatt ttcttgaacc tgaagtcaat ttggatgatt
     1381 ccatagatat tcagcaacag gatatggata tagatgaagg gcaagatgga gtggaagagg
     1441 aggtctttga acaagaagct aagaaagtgg cggttcgctc aagatcaaga acacattcac
     1501 gatctcgttc aagatcacca agaaaacgaa ggtctaggtc acggtctggc tctagaaagc
     1561 gtaaacacag aaagcgatca cgctcccgct caagagaaag aaagaggaaa tcatcacggt
     1621 cgtattcaag tgaaaggaga gccagagaaa gggagaaaga acgacagaaa aagggattac
     1681 ctccaattag atctaaaaca ctaagtgtat gtagtactac tctctgggtt gggcaagtgg
     1741 acaagaaggc aacacagcaa gacttaacca acctgtttga agagtttgga cagattgaat
     1801 ccattaatat gattcctccg cgaggctgtg cttatgtctg catggttcat cgacaagatg
     1861 catttcgagc tcttcagaaa ctcagttctg gatcatataa aattgggtcc aaggtcatta
     1921 agatcgcttg ggctttaaac aaaggtgtaa aaacagaata caaacaattc tgggatgtgg
     1981 atcttggagt tacatatata ccatgggaaa aagttaaagt ggatgacttg gaaggttttg
     2041 cagaaggagg catgattgat caggagactg taaatactga gtgggaaact gtgaaaagct
     2101 cagaacctgt taaagagacg gtccagacaa ctcagagccc aactccagtt gaaaaggaga
     2161 cagtggtcac aacccaggca gaggttttcc ctcctcctgt tgctatgttg cagattccag
     2221 tggcgccagc cgtgcctaca gttagtttag tcccaccagc atttcctgtg tcgatgccgg
     2281 ttcctcctcc tggattcagt ccaatccctc cacctccttt tttaagagca agttttaacc
     2341 cttcacaacc accacctggt ttcatgccgc ctccagttcc cccacctgtt gtgccacccc
     2401 ctacgattcc accagtagta ccaacatctt tagtgcagcc gtcattatcc atgacaccgg
     2461 aaactgtgaa agatgttgga tttggtagcc ttgttatacc aggcggttct gttgccagca
     2521 atcttgctac ttccgctctg ccagctggaa atgtttttaa tgctccaact aaacaggcag
     2581 agcctgaaga aaaagtacct catcttatag accaccagat ttcttctggt gaaaacacca
     2641 gatcagtgat tccaaatgat atttcaagta atgctgcaat tttaggagga cagccgccaa
     2701 atgtgacaag caattctgga attctgggag tccaaagacc aaatgtatca agtaattctg
     2761 aaattcttgg ggtccggcca tctaatgttt ccagtagttc tgggattatt gcagcccaac
     2821 caccaaatat tctaaataac tctggaatat tgggaataca gccacccagt gtgtcaaata
     2881 gttctggact tttgggagtg ctacccccaa atatacctaa caattctgga cttgtaggag
     2941 tacagccacc aaatgttcca aatactcctg gacttctggg aacacagcca ccagctggac
     3001 ctcaaaactt acccccttta agtatcccta atcaaaggat gcccacaatg ccaatgttag
     3061 acattcgtcc gggactaata ccacaggcac ctgggccaag attcccttta atacagcctg
     3121 gaattccacc ccaacgggga atcccacccc catcggtact tgattcagct cttcatccac
     3181 caccccgtgg accttttcct ccaggagata tttttagtca accagaaaga ccttttttag
     3241 ctcctggaag acaaagcgta gacaatgtta ctaacccaga aaaaaggata ccacttggga
     3301 atgataacat tcaacaggaa ggagatagag attatcggtt tcctcctata gaaaccaggg
     3361 aaagcattag tagacctccc cctgtggatg ttagagatgt ggttgggcgg cctatagatc
     3421 caagagaagg tcctggacgg cctccactag atggtaggga tcattttgga agacctcctg
     3481 tagatataag agagaatctt gtgaggccag gtatagatca tcttggtcga agagaccact
     3541 ttggctttaa tccagagaag ccctgggggc atagaggaga ttttgatgag agagagcatc
     3601 gggttctacc ggtctatggt ggtccaaaag gcttacatga agaaagaggt agatttcggt
     3661 ctggaaacta tcgatttgat cctagaagtg gtccttggaa ccgaggattt ggacaagaag
     3721 ttcacagaga ttttgatgac cgcagaagac cctgggagag gcaaagggat agggatgaca
     3781 gagattttga tttctgcaga gaaatgaatg gaaatcgtct tggacgagac agaattcaaa
     3841 acacttgggt tccccctcct catgctcggg tttttgatta ttttgaaggg gccacttctc
     3901 aacgaaaagg tgataatgtg cctcaggtta atggtgaaaa tacagagaga catgctcagc
     3961 caccacctat accagtacag aatgatcctg aactttatga aaaactgaca tcttcaaatg
     4021 aaataaacaa ggagaagagt gacacagttg ctgatataga aagtgaacca gtggtagaaa
     4081 gcacagaaac tgaggggaca taaaacccag ctttcttgta caaagttggc attataagaa
     4141 agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta
     4201 tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct
     4261 ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat
     4321 catcatgcct cctctagacc agccaggaca gaaatgcctc gacttcgctg ctacccaagg
     4381 ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg aacccagtgg acataagcct
     4441 gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct
     4501 tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt
     4561 tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc
     4621 gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa
     4681 agttaaacat tatgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag
     4741 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg
     4801 cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa
     4861 ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc
     4921 ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca
     4981 ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca
     5041 ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa
     5101 aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg
     5161 ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc
     5221 ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg
     5281 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc
     5341 cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag
     5401 atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga
     5461 tcaccaaggt agtcggcaaa taaccctcga gccacccatg accaaaatcc cttaacgtga
     5521 gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     5581 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     5641 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     5701 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     5761 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     5821 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     5881 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     5941 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     6001 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     6061 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     6121 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     6181 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     6241 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     6301 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     6361 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     6421 actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc caggaagagt
     6481 ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt gatgcctggc
     6541 agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc
     6601 cgctcccggc gg
//
