LOCUS       sequence-238000-         767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_CD156_00118721
FEATURES             Location/Qualifiers
     source          1..767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(447..463)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(447..463)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(460..482)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    471..487
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(495..501,502..519,520..525))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    540..561
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(678..695)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttggtctcta atggaagttt tttctgctgg tgttgctgct actggtcaat tgtgttgtac
       61 taatggtgtt ccagatgatt ctccaacttg taaaaaattg ggtttgaact cttatgctgt
      121 tattccaact ttttctttta cttctgaaac tccagaaaaa aataaagaat tgactttttt
      181 ttctatgcaa tgtgaatctg ttagaagaaa tgatgaaaaa gctcatagag aagatccagg
      241 tagaaaaaat ggttgtgata ataaattgtt ggaaggtttg tttccagttg gtagagatgt
      301 taaagctttt gttccaagat ctactaatac tgttgaattg ggtccaactt ctaaagttaa
      361 tactatgttt gcttttattg gttgtgctgc ttgagcttag agaccaagtt ccatcccaat
      421 ggcgcgccga gcttggctcg agcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
      481 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
      541 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
      601 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
      661 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
      721 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatc
//
