LOCUS       sequence-238002-         958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_CD156_00122701
FEATURES             Location/Qualifiers
     source          1..958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(378..394)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(378..394)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(391..413)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    402..418
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(426..432,433..450,451..456))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    471..492
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(609..626)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(860..879)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttggtctcta atgtggactg aaaaatgtat tattactatt agaaaaaatg atccagcttt
       61 gggtaaacat actttgtatg atatggtttc tttgccacca gattctgatt atacttttgg
      121 taaatttcca ccagctactg ctggtgttac tattggtgtt gatgatactt gtagagctgg
      181 tttggttgtt ggtgatttga atccaccata tgattataga ggtatggaaa atccatttta
      241 tggtttgact ggtgttgaat tggttagaca attgcatgat tggagagaaa atccagaaaa
      301 tgttattaat ttttctttga gatgagctta gagaccaagt tccatcccaa tggcgcgccg
      361 agcttggctc gagcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
      421 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
      481 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
      541 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
      601 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
      661 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
      721 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
      781 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
      841 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
      901 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaa
//
