LOCUS       sequence-238003-        5124 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Intermediate plasmid 1 to build tobacco etch virus recombinant 
            clones.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Daros JA
  TITLE     Use of Potyvirus Vectors to Produce Carotenoids in Plants.
  JOURNAL   Methods Mol Biol. 2020;2083:303-312. doi: 
            10.1007/978-1-4939-9952-1_23.
  PUBMED    31745931
REFERENCE   2  (bases 1 to 5124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2020"; volume: "2083:303-312. doi"; pages: " 
            10.1007/978-1-4939-9952-1_23"
COMMENT     Sequence Label: pTV1
FEATURES             Location/Qualifiers
     source          1..5124
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..47
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     primer_bind     complement(3409..3426)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3543..4088)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(3623..3642)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4106..4765)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4680..4699
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     join(5116..5124,1..12)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggaaaa taacaaatct
       61 caacacaaca tatacaaaac aaacgaatct caagcaatca agcattctac ttctattgca
      121 gcaatttaaa tcatttcttt taaagcaaaa gcaattttct gaaaattttc accatttacg
      181 aacgatagcc atggcactca tctttggcac agtcaacgct aacatcctga aggaagtgtt
      241 cggtggagct cgtatggctt gcgttaccag cgcacatatg gctggagcga atggaagcat
      301 tttgaagaag gcagaagaaa cctctcgtgc aatcatgcac aaaccagtga tcttcggaga
      361 agactacatt accgaggcag acttgcctta cacaccactc catttagagg tcgatgctga
      421 aatggagcgg atgtattatc ttggtcgtcg cgcgctcacc catggcaaga gacgcaaagt
      481 ttctgtgaat aacaagagga acaggagaag gaaagtggcc aaaacgtacg tggggcgtga
      541 ttccattgtt gagaagattg tagtgcccca caccgagaga aaggttgata ccacagcagc
      601 agtggaagac atttgcaatg aagctaccac tcaacttgtg cataatagta tgccaaagcg
      661 taagaagcag aaaaacttct tgcccgccac ttcactaagt aacgtgtatg cccaaacttg
      721 gagcatagtg cgcaaacgcc atatgcaggt ggagatcatt agcaagaaga gcgtccgagc
      781 gagggtcaag agatttgagg gctcggtgca attgttcgca agtgtgcgtc acatgtatgg
      841 cgagaggaaa agggtggact tacgtattga caactggcag caagagacac ttctagacct
      901 tgctaaaaga tttaagaatg agagagtgga tcaatcgaag ctcacttttg gttcaagtgg
      961 cctagttttg aggcaaggct cgtacggacc tgcgcattgg tatcgacatg gtatgttcat
     1021 tgtacgcggt cggtcggatg ggatgttggt ggatgctcgt gcgaaggtaa cgttcgctgt
     1081 ttgtcactca atgacacatt atagcgacaa atcaatctct gaggcattct tcataccata
     1141 ctctaagaaa ttcttggagt tgaggccaga tggaatctcc catgagtgta caagaggagt
     1201 atcagttgag cggtgcggtg aggtggctgc aatcctgaca caagcacttt caccgtgtgg
     1261 taagatcaca tgcaaacgtt gcatggttga aacacctgac attgttgagg gtgagtcggg
     1321 agacagtgtc accaaccaag gtaagctcct agcaatgctg aaagaacagt atccagattt
     1381 cccaatggcc gagaaactac tcacaaggtt tttgcaacag aaatcactag taaatacaaa
     1441 tttgacagcc tgcgtgagcg tcaaacaact cattggtgac cgcaaacaag ctccattcac
     1501 acacgtactg gctgtcagcg aaattctgtt taaaggcaat aaactaacag gggccgatct
     1561 cgaagaggca agcacacata tgcttgaaat agcaaggttc ttgaacaatc gcactgaaaa
     1621 tatgcgcatt ggccaccttg gttctttcag aaataaaatc tcatcgaagg cccatgtgaa
     1681 taacgcactc atgtgtgata atcaacttga tcagaatggg aattttattt ggggactaag
     1741 gggtgcacac gcaaagaggt ttcttaaagg atttttcact gagattgacc caaatgaagg
     1801 atacgataag tatgttatca ggaaacatat caggggtagc agaaagctag caattggcaa
     1861 tttgataatg tcaactgact tccagacgct caggcaacaa attcaaggcg aaactattga
     1921 gcgtaaagaa attgggaatc actgcatttc aatgcggaat ggtaattacg tgtacccatg
     1981 ttgttgtgtt actcttgaag atggtaaggc tcaatattcg gatctaaagc atccaacgaa
     2041 gagacatctg gtcattggca actctggcga ttcaaagtac ctagaccttc cagttctcaa
     2101 tgaagagaaa atgtatatag ctaatgaagg ttattgctac atgaacattt tctttgctct
     2161 actagtgaat gtcaaggaag aggatgcaaa ggacttcacc aagtttataa gggacacaat
     2221 tgttccaaag cttggagcgt ggccaacaat gcaagatgtt gcaactgcat gctacttact
     2281 ttccattctt tacccagatg tcctgagtgc tgaattaccc agaattttgg ttgatcatga
     2341 caacaaaaca atgcatgttt tggattcgta tgggtctaga acgacaggat accacatgtt
     2401 gaaaatgaac acaacatccc agctaattga attcgttcat tcaggtttgg aatccgaaat
     2461 gaaaacttac aatgttggag ggatgaaccg agatatggtc acacaaggtg caattgagat
     2521 gttgatcaag tccatataca aaccacatct catgaagcag ttacttgagg aggagccata
     2581 cataattgtc ctggcaatag tctccccttc aattttaatt gccatgtaca actctggaac
     2641 ttttgagcag gcgttacaaa tgtggttgcc aaatacaatg aggttagcta acctcgctgc
     2701 catcttgtca gccttggcgc aaaagttaac tttggcagac ttgttcgtcc agcagcgtaa
     2761 tttgattaat gagtatgcgc aggtaatttt ggacaatctg attgacggtg tcagggttaa
     2821 ccattcgcta tccctagcaa tggaaattgt tactattaag ctggccaccc aagagatgga
     2881 catggcgttg agggaaggtg gctatgctgt gacctctgaa aaggtgcatg aaatgttgga
     2941 aaaaaactat gtaaaggctt tgaaggatgc atgggacgaa ttaacttggt tggaaaaatt
     3001 ctccgcaatc aggcattcaa gaaagctctt gaaatttggg cgaaagcctt taatcatgaa
     3061 aaacaccgta gattgcggcg gacatataga cttgtctgtg aaatcgcttt tcaagttcca
     3121 cttggaactc ctgaagggaa ccatctcaag agccgtaaat ggtggtgcaa gaaaggtaag
     3181 agtagcgaag aatgccatga caaaaggggt ttttctcaaa atctacagca tgcttcctga
     3241 cgtcggcgcc taaggggcga gatctggcgg ggcccgcgct agcggagtgt atactggctt
     3301 actatgttgg cactgatgag ggtgtcagtg aagtgcttca tgtggcagga gaaaaaaggc
     3361 tgcaccggtg cgtcagcaga atatgtgata caggatatat tccgcttcct cgctcactga
     3421 ctcgctacgc tcggtcgttc gactgcggcg agcggaaatg gcttacgaac ggggcggaga
     3481 tttcctggaa gatgccagga agatacttaa cagggaagtg agagggccgc ggcaaagccg
     3541 tttttccata ggctccgccc ccctgacaag catcacgaaa tctgacgctc aaatcagtgg
     3601 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggcgg ctccctcgtg
     3661 cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg
     3721 tctcattcca cgcctgacac tcagttccgg gtaggcagtt cgctccaagc tggactgtat
     3781 gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     3841 caacccggaa agacatgcaa aagcaccact ggcagcagcc actggtaatt gatttagagg
     3901 agttagtctt gaagtcatgc gccggttaag gctaaactga aaggacaagt tttggtgact
     3961 gcgctcctcc aagccagtta cctcggttca aagagttggt agctcagaga accttcgaaa
     4021 aaccgccctg caaggcggtt ttttcgtttt cagagcaaga gattacgcgc agaccaaaac
     4081 gatctcaaga agatcatctt attaattacg ccccgccctg ccactcatcg cagtactgtt
     4141 gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa
     4201 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg
     4261 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg
     4321 gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt
     4381 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt
     4441 attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt
     4501 gaacactatc ccatatcacc agctcaccgt ctttcattgc catacggaat tccggatgag
     4561 cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct
     4621 ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag
     4681 caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg
     4741 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact
     4801 caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa cccgggggcg
     4861 gcggccgcga ttccattgcc cagctatctg tcactttatt gtgaagatag tggaaaagga
     4921 aggtggctcc tacaaatgcc atcattgcga taaaggaaag gccatcgttg aagatgcctc
     4981 tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga
     5041 cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat atctccactg acgtaaggga
     5101 tgacgcacaa tcccactatc cttc
//
