LOCUS       sequence-238004-         707 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_CD156_00123591
FEATURES             Location/Qualifiers
     source          1..707
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(621..637)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(621..637)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(634..656)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    645..661
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(669..675,676..693,694..699))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 ttggtctcta agctcattta tgaacaccag tccattcata accaaaatct tctggcaaat
       61 ccaaaataat atgagccaat tcatgcataa tagtagtatc ttgattagta gcagtaattt
      121 cagaagaaga ttctggttga ttaaaaaaat ttggacaaat aacaatttgt tctttagcaa
      181 ttctagcata acccaaaaca tttttataac cttgattttc tttacattta ccagtttcat
      241 cttcacaagt aatataaact tcatcttctt ttctagaaca ttcttcatac aattttttaa
      301 aattttgttc aaccaaatct tgaatatcag tagaatcagt tttaaaaata aattcaaaca
      361 attcttcatc agatttaaca gcatgaaaag catgttgagc caaaacagca caagattcca
      421 aagcacctct aatttcagaa gcttcagaag aagaacattg agaacctttt tgaatataag
      481 ctcttttatt caaagcacca tgaatatcat tagaaaattc tttataatta ttaattggtt
      541 gcaaaaaagt accagtatta ataaaagcca ttagagacca agttccatcc caatggcgcg
      601 ccgagcttgg ctcgagcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
      661 attccacaca acatacgagc cggaagcata aagtgtaaag cctgggg
//
