LOCUS       sequence-238010-         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_CD156_00138051
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(201..217)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(201..217)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(214..236)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    225..241
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(249..255,256..273,274..279))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    294..315
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(432..449)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(683..702)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttggtctcta agctcaaccc catctcaatc tagaaaaatg atgagctgga gttcttctca
       61 aaaacaaaga accatgagca attggaactt tagaagaata caaagtttga tcaatacaag
      121 caatccaatg ttcacattta cctctagcca ttagagacca agttccatcc caatggcgcg
      181 ccgagcttgg ctcgagcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
      241 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
      301 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
      361 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
      421 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
      481 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
      541 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
      601 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      661 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      721 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
      781 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
      841 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
      901 aactatcgtc ttgagtccaa cccggtaaga
//
