LOCUS       sequence-238014-         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_CD156_AVR-Pib_70
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(223..239)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(223..239)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(236..258)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    247..263
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(271..277,278..295,296..301))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    316..337
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(454..471)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(705..724)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttggtctcta atgactgaag ttactatttt gaaaaaaggt gaaagaattg cttgggttaa
       61 tgttgaacca ggttcttcta aatctgttaa tattagaggt aaatatttta ctgtttctgt
      121 ttctgattct ggtgaaaaat ctatttctgg ttctaaatat actgttgaat gagcttagag
      181 accaagttat cccaatggcg cgccgagctt ggctcgagca tggtcatagc tgtttcctgt
      241 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
      301 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
      361 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
      421 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
      481 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
      541 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
      601 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
      661 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
      721 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
      781 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gt
//
