LOCUS       sequence-238018-         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_FR13_00003821
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(659..675)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(659..675)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(672..694)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    683..699
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(707..713,714..731,732..737))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    752..773
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ttggtctcta atgactgaac aaggttcttc tgatcaaaaa attagaaatt tggaattgga
       61 aggttctcca gaagttagaa cttctagatc ttatgaagct gataatgttg gtagagctaa
      121 aactgttact ttgccatctc atcatagaat cccaaatgtt ccaaatatgt tttctggtag
      181 agctagagct gctgctggta aaccaacttc tgaagaagat ggtggtaaag ctattgaaag
      241 aagaggtgct gatagaaaag gtaaaggtat tttgaactct ttgatgccac caaaagtttc
      301 taatagacca ttgccagttg atttgccagt tgaacaacca cattatcatc aatattctgc
      361 tgattctgat tattgggatt ctgctccaga aaggagacca aaatctccaa gaccaagaat
      421 tactcaatgg tgtaaagttg aattggttaa aggtagagaa ccacaaggta tggttaaaca
      481 aaattgggat aaaacttttg ttattaaagg ttttgaattt aaattgtctc atgattgtac
      541 tattgttgct ggtagacatg aagctgaaag agctggttat gctgttttgt ctgctgctta
      601 taaatgagct tagagaccaa gttcatccca atggcgcgcc gagcttggct cgagcatggt
      661 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
      721 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
      781 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
      841 gccaacgcgc ggggagaggc ggtttgcgta ttg
//
