LOCUS       sequence-238020-         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_FR13_00004061
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(402..418)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(402..418)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(415..437)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    426..442
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(450..456,457..474,475..480))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    495..516
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(633..650)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttggtctcta atgagagaaa atggtcatgc tgataatgtt aaattggctc ataataatgc
       61 taatttgtct gataaaactt atcattattc tacttatgaa ttgtctaatg gtgaatgggt
      121 tcatagacca gaatattcta aaggtactaa accaggtggt tatgaaggta ttttgggtag
      181 accatatttt attcatttgg ataaatattg taatccatct tatccagatt ctaaaactgt
      241 ttatattaga gttcataatg atgatgttat taattgtgct tattggactg gttttccaat
      301 tgaatttatt gaaaatcaaa ttgctaaagg tagagaagct gaaatttgag cttagagacc
      361 aagttccatc ccaatggcgc gccgagcttg gctcgagcat ggtcatagct gtttcctgtg
      421 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
      481 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
      541 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
      601 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
      661 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
      721 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
      781 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
      841 aatcgacgct caagtca
//
