LOCUS       sequence-238021-         743 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_FR13_00013421
FEATURES             Location/Qualifiers
     source          1..743
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(387..403)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(387..403)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(400..422)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    411..427
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(435..441,442..459,460..465))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    480..501
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(618..635)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttggtctcta atggaatctg atccacattt ttgtaaagtt tctgtttatt atcaagatga
       61 aaatactcca agatatcaat ctactaaaaa atttgctgtt ccaccagcta aagttccaat
      121 tttggttggt tctagaatgc atgaagttac tgttgataaa gcttgtaaaa gaactggttg
      181 gaatggttgg tttggtttgc caagaggtta tactattgaa actgaaggtt ggagattgaa
      241 aggtgctcca gaagaaaatg aaattttgtc tttggttttg caattgaatg aaggtgtttt
      301 gagattgtct tctggtattg ctactagatc ttgagcttag agaccaagtt ccatcccaat
      361 ggcgcgccga gcttggctcg agcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
      421 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
      481 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
      541 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
      601 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
      661 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
      721 ggaaagaaca tgtgagcaaa agg
//
