LOCUS       sequence-238022-         958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_FR13_00019851
FEATURES             Location/Qualifiers
     source          1..958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(762..778)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(762..778)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(775..797)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    786..802
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(810..816,817..834,835..840))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    855..876
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ttggtctcta agctcatggt ttagaaacac ctcttggaaa caaagtttga tgaaaaccat
       61 aagaaccagc atcaccaact ctaacaacca taccacctgg aatatcacct tcaacaactc
      121 tagctttaca agttcttctt ctatatggac caacataatc tggttcagca ataatagtaa
      181 aataatttct aataaaagta gtttttttac caaaatcaat atttttagct tcagcagtat
      241 tagcaccatt agcagaagca aaaacaacat gacatctagt agcatctggt tctggatgca
      301 atttaaaagc atatggagaa gaaattggag acatttgtgg tttaacaata gaaacataat
      361 aacctcttgg aatttcacct ctccaaacat tacctctaca tggattatca ccaccttgtt
      421 cccattccaa tctaacagac aatcttggac ctggtggaat agccaaaaat ttaacagatt
      481 ctttataatt agcatatcta gtttcttgaa cttgattatc tttcaacaac caaacttcac
      541 aatctttttt caatctttga acttctggtt caccagtaaa atgagacaaa taagttggtt
      601 ccaaatcata ttcttgagtt ggttcatgag aagtagcagc ttgaccagaa ttataagaag
      661 cagtattacc tctatgacca taagcattac cccaattcat tggtggattc attagagacc
      721 aagttccatc ccaatggcgc gccgagcttg gctcgagcat ggtcatagct gtttcctgtg
      781 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
      841 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
      901 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcgggga
//
