LOCUS       sequence-238025-       12122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of EGFR H835L.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12122)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-EGFR-H835L
FEATURES             Location/Qualifiers
     source          1..12122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    123..144
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(159..164,165..182,183..189)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    197..213
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     202..224
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     221..237
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     221..237
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     287..306
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             498..1131
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1178..1303
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1800..2033
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2218..2262
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2555..2672
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2720..3898
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2951..3889
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3846..3866
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3918..3942
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(3945..4016,4017..5879,5880..5948,5949..7577)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVLRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     protein_bind    complement(7579..7603)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        7611..7629
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7654..7673)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7655..7673)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(7679..7699)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             7753..7794
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    7815..8382
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    7832..8384
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7976..7993)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8203..8222
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8396..8995
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8396..8415)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8892..8912
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9009..9597
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9062..9082)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9480..9491)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9672..9905
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(10024..10042)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10110..10214
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10215..10283,10284..11075)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      11246..11834
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11735..11754
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11988..12005
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
       61 aaaagctccc cgtggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
      121 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
      181 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgacatg
      241 attacgaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
      301 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat
      361 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc
      421 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt
      481 actacaaact tagtagttgg aagggctaat tcactcccaa agaagacaag atatccttga
      541 tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca caccagggcc
      601 aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga
      661 taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca
      721 tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt
      781 tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg
      841 ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga
      901 gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct
      961 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     1021 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     1081 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     1141 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     1201 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     1261 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     1321 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     1381 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     1441 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     1501 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     1561 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     1621 taagaccacc gcacagcaag cggccggccg ctgatcttca gacctggagg aggagatatg
     1681 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1741 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1801 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1861 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1921 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1981 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     2041 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     2101 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2161 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2221 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2281 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2341 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2401 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2461 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat
     2521 cgccgaattc acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
     2581 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     2641 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
     2701 cagtttggac tagtcgtgag gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
     2761 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg
     2821 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     2881 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     2941 cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg
     3001 cccttgcgtg ccttgaatta cttccacctg gctgcagtac gtgattcttg atcccgagct
     3061 tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg
     3121 tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct
     3181 tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc
     3241 tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg
     3301 tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc
     3361 ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg
     3421 ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag
     3481 gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc
     3541 agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca
     3601 aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc
     3661 gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg
     3721 ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc
     3781 agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt
     3841 cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgaag
     3901 cggccctgca gatatcaaca agtttgtaca aaaaagcagg caccatgcga ccctccggga
     3961 cggccggggc agcgctcctg gcgctgctgg ctgcgctctg cccggcgagt cgggctctgg
     4021 aggaaaagaa agtttgccaa ggcacgagta acaagctcac gcagttgggc acttttgaag
     4081 atcattttct cagcctccag aggatgttca ataactgtga ggtggtcctt gggaatttgg
     4141 aaattaccta tgtgcagagg aattatgatc tttccttctt aaagaccatc caggaggtgg
     4201 ctggttatgt cctcattgcc ctcaacacag tggagcgaat tcctttggaa aacctgcaga
     4261 tcatcagagg aaatatgtac tacgaaaatt cctatgcctt agcagtctta tctaactatg
     4321 atgcaaataa aaccggactg aaggagctgc ccatgagaaa tttacaggaa atcctgcatg
     4381 gcgccgtgcg gttcagcaac aaccctgccc tgtgcaacgt ggagagcatc cagtggcggg
     4441 acatagtcag cagtgacttt ctcagcaaca tgtcgatgga cttccagaac cacctgggca
     4501 gctgccaaaa gtgtgatcca agctgtccca atgggagctg ctggggtgca ggagaggaga
     4561 actgccagaa actgaccaaa atcatctgtg cccagcagtg ctccgggcgc tgccgtggca
     4621 agtcccccag tgactgctgc cacaaccagt gtgctgcagg ctgcacaggc ccccgggaga
     4681 gcgactgcct ggtctgccgc aaattccgag acgaagccac gtgcaaggac acctgccccc
     4741 cactcatgct ctacaacccc accacgtacc agatggatgt gaaccccgag ggcaaataca
     4801 gctttggtgc cacctgcgtg aagaagtgtc cccgtaatta tgtggtgaca gatcacggct
     4861 cgtgcgtccg agcctgtggg gccgacagct atgagatgga ggaagacggc gtccgcaagt
     4921 gtaagaagtg cgaagggcct tgccgcaaag tgtgtaacgg aataggtatt ggtgaattta
     4981 aagactcact ctccataaat gctacgaata ttaaacactt caaaaactgc acctccatca
     5041 gtggcgatct ccacatcctg ccggtggcat ttaggggtga ctccttcaca catactcctc
     5101 ctctggatcc acaggaactg gatattctga aaaccgtaaa ggaaatcaca gggtttttgc
     5161 tgattcaggc ttggcctgaa aacaggacgg acctccatgc ctttgagaac ctagaaatca
     5221 tacgcggcag gaccaagcaa catggtcagt tttctcttgc agtcgtcagc ctgaacataa
     5281 catccttggg attacgctcc ctcaaggaga taagtgatgg agatgtgata atttcaggaa
     5341 acaaaaattt gtgctatgca aatacaataa actggaaaaa actgtttggg acctccggtc
     5401 agaaaaccaa aattataagc aacagaggtg aaaacagctg caaggccaca ggccaggtct
     5461 gccatgcctt gtgctccccc gagggctgct ggggcccgga gcccagggac tgcgtctctt
     5521 gccggaatgt cagccgaggc agggaatgcg tggacaagtg caaccttctg gagggtgagc
     5581 caagggagtt tgtggagaac tctgagtgca tacagtgcca cccagagtgc ctgcctcagg
     5641 ccatgaacat cacctgcaca ggacggggac cagacaactg tatccagtgt gcccactaca
     5701 ttgacggccc ccactgcgtc aagacctgcc cggcaggagt catgggagaa aacaacaccc
     5761 tggtctggaa gtacgcagac gccggccatg tgtgccacct gtgccatcca aactgcacct
     5821 acggatgcac tgggccaggt cttgaaggct gtccaacgaa tgggcctaag atcccgtcca
     5881 tcgccactgg gatggtgggg gccctcctct tgctgctggt ggtggccctg gggatcggcc
     5941 tcttcatgcg aaggcgccac atcgttcgga agcgcacgct gcggaggctg ctgcaggaga
     6001 gggagcttgt ggagcctctt acacccagtg gagaagctcc caaccaagct ctcttgagga
     6061 tcttgaagga aactgaattc aaaaagatca aagtgctggg ctccggtgcg ttcggcacgg
     6121 tgtataaggg actctggatc ccagaaggtg agaaagttaa aattcccgtc gctatcaagg
     6181 aattaagaga agcaacatct ccgaaagcca acaaggaaat cctcgatgaa gcctacgtga
     6241 tggccagcgt ggacaacccc cacgtgtgcc gcctgctggg catctgcctc acctccaccg
     6301 tgcagctcat cacgcagctc atgcccttcg gctgcctcct ggactatgtc cgggaacaca
     6361 aagacaatat tggctcccag tacctgctca actggtgtgt gcagatcgca aagggcatga
     6421 actacttgga ggaccgtcgc ttggtgctcc gcgacctggc agccaggaac gtactggtga
     6481 aaacaccgca gcatgtcaag atcacagatt ttgggctggc caaactgctg ggtgcggaag
     6541 agaaagaata ccatgcagaa ggaggcaaag tgcctatcaa gtggatggca ttggaatcaa
     6601 ttttacacag aatctatacc caccagagtg atgtctggag ctacggggtg accgtttggg
     6661 agttgatgac ctttggatcc aagccatatg acggaatccc tgccagcgag atctcctcca
     6721 tcctggagaa aggagaacgc ctccctcagc cacccatatg taccatcgat gtctacatga
     6781 tcatggtcaa gtgctggatg atagacgcag atagtcgccc aaagttccgt gagttgatca
     6841 tcgaattctc caaaatggcc cgagaccccc agcgctacct tgtcattcag ggggatgaaa
     6901 gaatgcattt gccaagtcct acagactcca acttctaccg tgccctgatg gatgaagaag
     6961 acatggacga cgtggtggat gccgacgagt acctcatccc acagcagggc ttcttcagca
     7021 gcccctccac gtcacggact cccctcctga gctctctgag tgcaaccagc aacaattcca
     7081 ccgtggcttg cattgataga aatgggctgc aaagctgtcc catcaaggaa gacagcttct
     7141 tgcagcgata cagctcagac cccacaggcg ccttgactga ggacagcata gacgacacct
     7201 tcctcccagt gcctgaatac ataaaccagt ccgttcccaa aaggcccgct ggctctgtgc
     7261 agaatcctgt ctatcacaat cagcctctga accccgcgcc cagcagagac ccacactacc
     7321 aggaccccca cagcactgca gtgggcaacc ccgagtatct caacactgtc cagcccacct
     7381 gtgtcaacag cacattcgac agccctgccc actgggccca gaaaggcagc caccaaatta
     7441 gcctggacaa ccctgactac cagcaggact tctttcccaa ggaagccaag ccaaatggca
     7501 tctttaaggg ctccacagct gaaaatgcag aatacctaag ggtcgcgcca caaagcagtg
     7561 aatttattgg agcatagaac ccagctttct tgtacaaagt ggtgatatcc aattaaccct
     7621 cactaaaggt taagaagaat tgtatatgag agtccctata gtgagtcgta ttaccaccca
     7681 acttttctat acaaagtggt tgatatccag cacagtggcg gccgctcgag tctagagggc
     7741 ccgcggttcg aaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc
     7801 ggttagtaat gagatccctc ccccccccct aacgttactg gccgaagccg cttggaataa
     7861 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
     7921 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
     7981 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
     8041 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
     8101 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
     8161 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
     8221 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
     8281 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg
     8341 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca cacatatgac
     8401 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     8461 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     8521 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     8581 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     8641 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     8701 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     8761 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     8821 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     8881 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     8941 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctaaatcga
     9001 tagatcctaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     9061 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     9121 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     9181 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     9241 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     9301 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     9361 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     9421 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     9481 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     9541 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     9601 agatccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     9661 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
     9721 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     9781 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     9841 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     9901 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     9961 atcagagagt gagaggcccg ggttaattaa ggaaagggct agatcattct tgaagacgaa
    10021 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
    10081 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
    10141 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
    10201 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
    10261 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
    10321 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
    10381 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
    10441 gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt
    10501 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
    10561 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
    10621 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
    10681 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
    10741 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
    10801 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
    10861 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
    10921 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
    10981 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
    11041 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
    11101 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
    11161 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
    11221 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
    11281 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
    11341 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
    11401 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
    11461 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
    11521 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
    11581 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
    11641 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
    11701 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
    11761 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
    11821 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
    11881 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
    11941 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
    12001 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
    12061 attaatgcag caagctcatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
    12121 ct
//
