LOCUS       sequence-238028-        2124 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Myofilament localised red genetically encoded calcium sensor in 
            mammalian expression vector used for AdEasy adenoviral DNA 
            recombination..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Sparrow AJ, Sievert K, Patel S, Chang YF, Broyles CN, Brook FA, 
            Watkins H, Geeves MA, Redwood CS, Robinson P, Daniels MJ
  TITLE     Measurement of Myofilament-Localised Calcium Dynamics in Adult 
            Cardiomyocytes and the Effect of Hypertrophic Cardiomyopathy 
            Mutations.
  JOURNAL   Circ Res. 2019 Feb 8. doi: 10.1161/CIRCRESAHA.118.314600.
  PUBMED    30732532
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Circ Res. 
            2019 Feb 8. doi: 10.1161/CIRCRESAHA.118.314600."
COMMENT     Sequence Label: pShuttle CMV RGECO-TnT
FEATURES             Location/Qualifiers
     source          1..2124
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..72
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKAGHAVRAIGRLSS"
     primer_bind     768..787
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atggtagact catcacgtcg taagtggaat aaggcaggtc acgcagtcag agctataggt
       61 cggctgagct cacccgtggt ttccgagcgg atgtaccccg aggacggcgc cctcaagagc
      121 gagatcaaga aggggctgag gctgaaggac ggcggccact acgccgccga ggtcaagacc
      181 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acatcgtaga catcaagttg
      241 gacatcgtgt cccacaacga ggactacacc atcgtggaac agtgcgaacg cgccgagggc
      301 cgccactcca ccggcggcat ggacgagcta tacaagggag gtacaggcgg gagtctggtg
      361 agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac
      421 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
      481 tacgaggcct ttcagaccgc taagctgaag gtgaccaagg gtggccccct gcccttcgcc
      541 tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacattaa gcacccagcc
      601 gacatccccg actacttcaa gctgtccttc cccgagggct tcaggtggga gcgcgtgatg
      661 aacttcgagg acggcggcat tattcacgtt aaccaggact cctccctgca ggacggcgta
      721 ttcatctaca aggtgaagct gcgcggcacc aacttccccc ccgacggccc cgtaatgcag
      781 aagaagacca tgggctggga ggctacgcgt gaccaactga ctgaggagca gatcgcagaa
      841 tttaaagagg ctttctccct atttgacaag gacggggatg ggacgataac aaccaaggag
      901 ctggggacgg tgatgcggtc tctggggcag aaccccacag aagcagagct gcaggacatg
      961 atcaatgaag tagatgccga cggtgacggc acattcgact tccctgagtt cctgacgatg
     1021 atggcaagaa aaatgaatga cacagacagt gaagaggaaa ttagagaagc gttccgcgtg
     1081 tttgataagg acggcaatgg ctacatcggc gcagcagagc ttcgccacgt gatgacagac
     1141 cttggagaga agttaacaga tgaggaggtt gatgaaatga tcagggtagc agacatcgat
     1201 ggggatggtc aggtaaacta cgaagagttt gtccaaatga tgacagcgaa gggatccatg
     1261 tctgacatag aagaggtggt ggaagagtac gaggaggagg agcaggaaga agcagctgtt
     1321 gaagagcagg aggaggcagc ggaagaggat gctgaagcag aggctgagac cgaggagacc
     1381 agggcagaag aagatgaaga agaagaggaa gcaaaggagg ctgaagatgg cccaatggag
     1441 gagtccaaac caaagcccag gtcgttcatg cccaacttgg tgcctcccaa gatccccgat
     1501 ggagagagag tggactttga tgacatccac cggaagcgca tggagaagga cctgaatgag
     1561 ttgcaggcgc tgattgaggc tcactttgag aacaggaaga aagaggagga ggagctcgtt
     1621 tctctcaaag acaggatcga gagacgtcgg gcagagcggg ccgagcagca gcgcatccgg
     1681 aatgagcggg agaaggagcg gcagaaccgc ctggctgaag agagggctcg acgagaggag
     1741 gaggagaacc gtcgtaaggc tgaggatgag gcccggaaga agaaggcttt gtccaacatg
     1801 atgcattttg ggggttacat ccagaagcag gcccagacag agcggaaaag tgggaagagg
     1861 cagactgagc gggaaaagaa gaagaagatt ctggctgagc gtcgtaaggt gctggccatt
     1921 gaccacctga atgaagatca gctgagggag aaggccaagg agctgtggca gagcatctat
     1981 aacttggagg cagagaagtt cgacctgcag gagaagttca agcagcagaa atatgagatc
     2041 aatgttctcc gaaacaggat caacgataac cagaaagtct ccaagacccg cgggaaggct
     2101 aaagtcaccg ggcgctggaa atag
//
