LOCUS       sequence-238033-        6347 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Labels centriolar satellites in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6347)
  AUTHORS   Takemaru et al.
  TITLE     Takemaru Lab Unpublished
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6347)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: GFP-hCCDC11
FEATURES             Location/Qualifiers
     source          1..6347
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             88..801
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(118..146)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     741..761
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(2375..2394)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2376..2394)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    2399..2533
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2423..2442)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2477..2496
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2646..2666)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2646..2664)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2685..2701)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2685..2701)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2698..2720)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2709..2725
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2733..2739,2740..2757,2758..2763))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2778..2799
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2916..2933)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3087..3675)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3167..3186)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3846..4637,4638..4706))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4469..4488
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4707..4811)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4837..5292)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4974..4995)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5186..5205
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5363..6347
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atctaccatg agtaaaggag aagaactttt cactggagtt
      121 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga
      181 gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga
      241 aaactacctg ttccatggcc aacacttgtc actactctca cttatggtgt tcaatgcttt
      301 tcaagatacc cagatcatat gaaacggcat gactttttca agagtgccat gcccgaaggt
      361 tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa
      421 gtcaagtttg aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa
      481 gaagatggaa acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac
      541 atcatggcag acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt
      601 gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc
      661 cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc
      721 aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat
      781 ggcatggatg aactatacaa aggatccatg tacagccagc ggtttggcac cgtacagcgg
      841 gaggttaagg gccccacccc caaagtggtg atcgtgagat ccaagcctcc taaaggccaa
      901 ggagctgagc accatctaga aagaatccga cgcagccatc agaagcataa tgctattttg
      961 gcttccatta agtcaagtga gcgggatcgc ttgaaagctg agtgggacca gcacaatgac
     1021 tgcaagattt tggacagcct tgtgcgagca agaatcaagg atgctgtgca agggtttatc
     1081 attaacattg aagaaagacg aaataagcta cgtgagcttt tagcattaga agaaaatgag
     1141 tattttacag aaatgcaatt gaagaaagaa accattgagg agaaaaaaga taggatgaga
     1201 gagaaaacta aattactaaa agagaagaat gaaaaagaga ggcaggattt tgtggctgaa
     1261 aagctagacc agcaattcag ggaacgctgt gaggagctcc gtgttgaatt gttatctatc
     1321 catcagaaga aggtgtgtga ggagcggaaa gcacagattg catttaatga ggagctgagc
     1381 aggcaaaagc tggtggaaga gcagatgttc tccaaactct gggaggaaga ccgattagcc
     1441 aaggaaaagc gagaagccca agaggcgagg agacagaaag agctgatgga gaacacacgc
     1501 ctggggctga atgcccagat caccagcatc aaggcacaaa ggcaggcgac acagctgctg
     1561 aaggaagagg aggcacgcct tgtggaaagt aacaacgcac agattaaaca tgagaatgaa
     1621 caggatatgc taaagaaaca gaaggcaaag caggaaacta ggaccatttt gcaaaaagcc
     1681 ctacaagaga ggatagaaca tattcagcag gaatacagag acgaacagga cttgaacatg
     1741 aagctcgtgc aaagggccct tcaagactta caggaagagg cagataaaaa gaaacaaaaa
     1801 agagaagata tgataagaga acagaagata taccataaat atttggcaca gagacgtgag
     1861 gaagaaaaag ctcaggagaa agaatttgac agaatattag aggaagacaa ggcaaagaag
     1921 ttggctgaga aggacaagga gctgagactt gaaaaggagg caaggagaca gcttgtggat
     1981 gaggtcatgt gtacaagaaa acttcaagtt caagaaaagt tgcaacgaga agctaaagaa
     2041 caggaagaac gtgctatgga acagaaacac ataaatgaaa gtcttaaaga acttaactgt
     2101 gaagagaagg agaattttgc aagacgccaa cgtttagccc aggagtacag gaagcaactt
     2161 cagatgcaaa tcgcctacca gcagcagtcc caagaagcag agaaggaaga gaaacgccga
     2221 gagtttgaag caggtgtagc agcaaacaag atgtgtttgg acaaggtcca ggaggtcctg
     2281 tccacccatc aagtgctgcc tcaaaacatt catcccatgc gcaaggcatg ccccagtaag
     2341 cttccaccgt agctcgagcc tctagattct gcagccctat agtgagtcgt attacgtaga
     2401 tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     2461 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     2521 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
     2581 gtgggaggtt ttttaattcg cggccgcggc gccaatgcat tgggcccggt acccagcttt
     2641 tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct
     2701 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccgggag cataaagtgt
     2761 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     2821 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     2881 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     2941 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     3001 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     3061 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     3121 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     3181 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     3241 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     3301 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     3361 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     3421 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     3481 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     3541 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     3601 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     3661 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     3721 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     3781 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     3841 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     3901 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     3961 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     4021 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     4081 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     4141 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     4201 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     4261 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     4321 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     4381 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     4441 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     4501 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     4561 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     4621 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     4681 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     4741 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     4801 ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt
     4861 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     4921 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     4981 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     5041 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     5101 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     5161 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     5221 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     5281 tacagggcgc gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     5341 gggcctcttc gctattacgc cagtcgaccg ccaattcaat atggcgtata tggactcatg
     5401 ccaattcaat atggtggatc tggacctgtg ccaattcaat atggcgtata tggactcgtg
     5461 ccaattcaat atggtggatc tggaccccag ccaattcaat atggcggact tggcaccatg
     5521 ccaattcaat atggcggacc tggcactgtg ccaactgggg aggggtctac ttggcacggt
     5581 gccaagtttg aggaggggtc ttggccctgt gccaagtccg ccatattgaa ttggcatggt
     5641 gccaataatg gcggccatat tggctatatg ccaggatcaa tatataggca atatccaata
     5701 tggccctatg ccaatatggc tattggccag gttcaatact atgtattggc cctatgccat
     5761 atagtattcc atatatgggt tttcctattg acgtagatag cccctcccaa tgggcggtcc
     5821 catataccat atatggggct tcctaatacc gcccatagcc actcccccat tgacgtcaat
     5881 ggtctctata tatggtcttt cctattgacg tcatatgggc ggtcctattg acgtatatgg
     5941 cgcctccccc attgacgtca attacggtaa atggcccgcc tggctcaatg cccattgacg
     6001 tcaataggac cacccaccat tgacgtcaat gggatggctc attgcccatt catatccgtt
     6061 ctcacgcccc ctattgacgt caatgacggt aaatggccca cttggcagta catcaatatc
     6121 tattaatagt aacttggcaa gtacattact attggaagta cgccagggta cattggcagt
     6181 actcccattg acgtcaatgg cggtaaatgg cccgcgatgg ctgccaagta catccccatt
     6241 gacgtcaatg gggaggggca atgacgcaaa tgggcgttcc attgacgtaa atgggcggta
     6301 ggcgtgccta atgggaggtc tatataagca atgctcgttt agggaac
//
