LOCUS       sequence-238039-        2144 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly AB part (low-strength Mutalik library 
            promoter; see DOI: 10.1038/nmeth.2404). Please see Supplemental 
            Documents for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2144)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: P34m
FEATURES             Location/Qualifiers
     source          1..2144
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     32..50
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(54..97)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    100..121
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    187..207
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(213..230)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(384..972)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(464..483)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1143..1934,1935..2003))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1766..1785
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2004..2108)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc agaatttcag
       61 ataaaaaaaa tccttagctt tcgctaagga tgatttctgg aattcgcggc cgcttctaga
      121 gactagtggg tctcaggagt tccctattaa tcatccggct cgtataatgt gtggatacta
      181 gagacctact agtagcggcc gctgcaggct tcctcgctca ctgactcgct gcgctcggtc
      241 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
      301 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
      361 aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga gcatcacaaa
      421 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
      481 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
      541 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
      601 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
      661 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
      721 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
      781 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
      841 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
      901 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
      961 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     1021 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     1081 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     1141 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     1201 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     1261 cccagtgctg caatgatacc gcgcgaccca cgctcaccgg ctccagattt atcagcaata
     1321 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     1381 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     1441 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     1501 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     1561 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     1621 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     1681 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     1741 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     1801 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     1861 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     1921 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     1981 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     2041 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     2101 gttccgcgca catttccccg aaaagtgcca cctgacgtct aaga
//
