LOCUS       sequence-238040-        2178 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly AB part for pRpa-AM (Adam Meyer's 
            improved pRPA promoter from https://doi.org/10.1101/285866, selected
            for dynamic range and low cross-sensitivity)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2178)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: P66m
FEATURES             Location/Qualifiers
     source          1..2178
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     27..44
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    50..70
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     misc_feature    170..191
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      194..237
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(241..259)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        327..431
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(432..500,501..1292)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(650..669)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1463..2051
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1952..1971
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcc tgcagcggcc
       61 gctactagta ggtctctagt atttattcga aagtaacaaa ccattttctt gcgtaatatt
      121 gtccgatcgg acaggtcagc caacgatcgt tctcctgaga cccactagtc tctagaagcg
      181 gccgcgaatt ccagaaatca tccttagcga aagctaagga ttttttttat ctgaaattct
      241 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
      301 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
      361 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      421 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      481 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      541 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      601 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      661 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      721 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      781 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      841 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      901 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      961 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     1021 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     1081 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     1141 agcgtgggtc gcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     1201 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     1261 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1321 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1381 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1441 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1501 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1561 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     1621 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1681 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1741 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1801 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1861 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1921 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1981 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     2041 gcctgtggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     2101 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     2161 ttgagtgagc tgataccg
//
