LOCUS       sequence-238041-        2819 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for Citrine (modified YFP; 
            variant of https://www.fpbase.org/protein/citrine/) - Please see 
            Supplemental Documents for annotated Genbank file.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2819)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C11m
FEATURES             Location/Qualifiers
     source          1..2819
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     21..39
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(43..86)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    89..110
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             126..845
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSRLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYKR"
     misc_feature    851..871
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(877..894)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1048..1636)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1128..1147)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1807..2598,2599..2667))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2430..2449
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2668..2772)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcatgacatt aacctataaa aataggcgta tcacgaggca gaatttcaga taaaaaaaat
       61 ccttagcttt cgctaaggat gatttctgga attcgcggcc gcttctagag actagtgggt
      121 ctcaaatgtc taaaggtgaa gaattattca ctggtgttgt cccaattttg gttgaattag
      181 atggtgatgt taatggtcac aaattttctg tctccggtga aggtgaaggt gatgctactt
      241 acggtaaatt gaccttaaaa tttatttgta ctactggtaa attgccagtt ccatggccaa
      301 ccttagtcac tactttaggt tatggtttga tgtgttttgc tagataccca gatcatatga
      361 aacaacatga ctttttcaag tctgccatgc cagaaggtta tgttcaagaa agaactattt
      421 ttttcaaaga tgacggtaac tacaagacca gagctgaagt caagtttgaa ggtgatacct
      481 tagttaatag aatcgaatta aaaggtattg attttaaaga agatggtaac attttaggtc
      541 acaaattgga atacaactat aactctcaca atgtttacat catggctgac aaacaaaaga
      601 atggtatcaa agttaacttc aaaattagac acaacattga agatggttct gttcaattag
      661 ctgaccatta tcaacaaaat actccaattg gtgatggtcc agtcttgtta ccagacaacc
      721 attacttatc ctatcaatct agattatcca aagatccaaa cgaaaagaga gatcacatgg
      781 tcttgttaga atttgttact gctgctggta ttacccatgg tatggatgaa ttgtacaaaa
      841 ggtagagacc tactagtagc ggccgctgca ggcttcctcg ctcactgact cgctgcgctc
      901 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
      961 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     1021 ccgtaaaaag gccgcgttgc tggcgttttt ccacaggctc cgcccccctg acgagcatca
     1081 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     1141 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     1201 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     1261 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     1321 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     1381 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     1441 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     1501 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     1561 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     1621 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     1681 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     1741 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     1801 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     1861 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     1921 tggccccagt gctgcaatga taccgcgcga cccacgctca ccggctccag atttatcagc
     1981 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     2041 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     2101 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     2161 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     2221 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     2281 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     2341 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     2401 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     2461 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     2521 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     2581 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     2641 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     2701 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     2761 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattatta
//
