LOCUS       sequence-238044-        2858 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for AiiA (AHL degradase; see doi:
            [10.1126/science.aaa3794]). Please see Supplemental Documents for 
            annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2858)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C33m
FEATURES             Location/Qualifiers
     source          1..2858
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     7..25
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(29..72)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    75..96
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    876..896
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(902..919)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1073..1661)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1153..1172)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1832..2623,2624..2692))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2455..2474
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2693..2797)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tataaaaata ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt agctttcgct
       61 aaggatgatt tctggaattc gcggccgctt ctagagacta gtgggtctca aatgacagta
      121 aagaaacttt atttcatccc agcaggtcgt tgcatgttgg atcattcgtc tgttaacagt
      181 gcgttaacac cggggaaact attaaacttg ccggtgtggt gttatctttt ggagacggaa
      241 gaaggtccta ttttagtaga cacaggtatg ccagaaagtg cagttaataa tgaagggctt
      301 tttaacggta catttgttga aggacagatc ttaccgaaaa tgactgaaga agatagaatc
      361 gtgaatatat taaagcgtgt agggtatgag ccggacgacc ttttatatat tattagttct
      421 cacttacatt ttgatcatgc aggaggaaac ggtgctttta caaatacacc aattattgtg
      481 cagcgaacgg aatatgaggc agcacttcat agagaagaat atatgaaaga atgtatatta
      541 ccgcatttga actacaaaat tattgaaggg gattatgaag tggtaccagg tgttcaatta
      601 ttgtatacgc caggtcattc tccaggccat cagtcgctat tcattgagac ggagcaatcc
      661 ggttcagttt tattaacgat tgatgcatcg tacacgaaag agaattttga agatgaagtg
      721 ccgttcgcag gatttgatcc agaattagct ttatcttcaa ttaaacgttt aaaagaagtt
      781 gtgaaaaaag agaaaccaat tattttcttt ggtcatgata tagagcagga aaagagttgt
      841 agagtgttcc cggaatatat ataaaggtag agacctacta gtagcggccg ctgcaggctt
      901 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
      961 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     1021 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccaca
     1081 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     1141 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     1201 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     1261 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     1321 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     1381 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     1441 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     1501 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     1561 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     1621 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     1681 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     1741 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     1801 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     1861 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     1921 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgcgacccac
     1981 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     2041 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     2101 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     2161 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     2221 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     2281 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     2341 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     2401 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     2461 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     2521 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     2581 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     2641 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     2701 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     2761 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     2821 ctgacgtcta agaaaccatt attatcatga cattaacc
//
