LOCUS       sequence-238052-         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_FR13_00057121
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(322..338)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(322..338)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(335..357)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    346..362
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(370..376,377..394,395..400))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    415..436
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(553..570)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(804..823)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cttggtctct aatgtttcca tgggatgaaa gagtttgtca agttactgtt ttttatccag
       61 gtaaagaaac tggtactttg atgccacatt tgatggaacc agaagaacca attgttattc
      121 caaaaattaa tggtaaaaga tattatgctg tttctgatgc taattgtaaa tataaaagat
      181 atgatgttga tccagaaggt aatccatggc catctggttt gagagttaaa ggtgttgctg
      241 ttaaattgtg gaaagaaaaa ttggattgag cttagagacc aagttccatc ccaatggcgc
      301 gccgagcttg gctcgagcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
      361 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
      421 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
      481 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
      541 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
      601 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
      661 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
      721 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
      781 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
      841 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
      901 aagcgtggcg ctttctcata gctcacgctg tag
//
