LOCUS       sequence-238055-         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_FR13_00067231
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(338..354)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(338..354)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(351..373)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    362..378
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(386..392,393..410,411..416))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    431..452
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(569..586)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(820..839)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttggtctcta agctcaaaca caagcaacaa aaccattagt agtaccagac aaacattgtt
       61 gtctattttt atcagtaaca aaagctttaa ccaatctacc agttggaaaa tcagtcaatc
      121 tatcacaacc acctttttga ccatcagcat gtggagtatc agctctaaaa ttagtagcac
      181 aaaaagagtt catacccaat ttagaacaag tttcagttgg atctggaaca ccttctttag
      241 aacaacaaat ttcacccaaa actggatcaa ctctatgaac tctttgcatt agagaccaag
      301 ttcatcccaa tggcgcgccg agcttggctc gagcatggtc atagctgttt cctgtgtgaa
      361 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
      421 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
      481 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
      541 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
      601 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
      661 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
      721 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
      781 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      841 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
      901 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
//
