LOCUS       sequence-238056-        2832 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for RhlR (Rhl quorum-sensing 
            receptor for C4-HSL, synonymously modified to remove internal BbsI 
            and BsaI cut sites)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2832)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C35m
FEATURES             Location/Qualifiers
     source          1..2832
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     14..32
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(36..79)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    82..103
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    857..877
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(883..900)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1054..1642)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1134..1153)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1813..2604,2605..2673))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2436..2455
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2674..2778)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 attaacctat aaaaataggc gtatcacgag gcagaatttc agataaaaaa aatccttagc
       61 tttcgctaag gatgatttct ggaattcgcg gccgcttcta gagactagtg ggtctcaaat
      121 gaggaatgac ggaggctttt tgctgtggtg ggacggtttg cgtagcgaga tgcagccgat
      181 ccacgacagc cagggcgtgt tcgccgtcct ggaaaaggaa gtgcggcgcc tgggcttcga
      241 ttactacgcc tatggcgtgc gccacacgat tcccttcacc cggcccaaaa ccgaggtcca
      301 tggcacctat cccaaggcct ggctggagcg ataccagatg cagaactacg gggccgtgga
      361 tccggcgatc ctcaacggcc tgcgctcctc ggaaatggtg gtctggagcg acagcctgtt
      421 cgaccagagc cggatgctct ggaacgaggc tcgcgattgg ggcctctgtg tcggcgcgac
      481 cttgccgatc cgcgcgccga acaatttgct cagcgtgctt tccgtggcgc gcgaccagca
      541 gaacatctcc agcttcgagc gcgaggaaat ccgcctgcgg ctgcgttgca tgatcgagtt
      601 gctgacccag aagctgaccg acctggagca tccgatgctg atgtccaacc cggtctgcct
      661 gagccatcgc gaacgcgaga tcctgcaatg gaccgccgac ggcaagagtt ccggggaaat
      721 cgccatcatc ctgagcatct ccgagagcac ggtgaacttc caccacaaga acatccagaa
      781 gaagttcgac gcgccgaaca agacgctggc tgccgcctac gccgcggcgc tgggtttaat
      841 ctaataggta gagacctact agtagcggcc gctgcaggct tcctcgctca ctgactcgct
      901 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
      961 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     1021 caggaaccgt aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga
     1081 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     1141 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     1201 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     1261 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     1321 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     1381 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     1441 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     1501 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     1561 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     1621 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     1681 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     1741 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     1801 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     1861 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     1921 accatctggc cccagtgctg caatgatacc gcgcgaccca cgctcaccgg ctccagattt
     1981 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     2041 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     2101 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     2161 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     2221 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     2281 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     2341 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     2401 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     2461 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     2521 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     2581 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     2641 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     2701 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     2761 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
     2821 tattatcatg ac
//
