LOCUS       sequence-238061-        2192 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly BC part for BCD14 (Medium expression 
            bi-cistronic RBS, engineered for downstream context-independence; 
            see https://doi.org/10.1038/nmeth.2404)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2192)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: U5m
FEATURES             Location/Qualifiers
     source          1..2192
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(70..174)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     242..260
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(264..307)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    310..331
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    445..465
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(471..488)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(642..1230)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(722..741)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1401..2192,1..69))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2024..2043
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
       61 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
      121 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
      181 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
      241 aaataggcgt atcacgaggc agaatttcag ataaaaaaaa tccttagctt tcgctaagga
      301 tgatttctgg aattcgcggc cgcttctaga gactagtggg tctcatactg ggcccaagtt
      361 cacttaaaaa ggagatcaac aatgaaagca attttcgtac tgaaacatct taatcatgcg
      421 gtggagggtt tctaatgaga gacctactag tagcggccgc tgcaggcttc ctcgctcact
      481 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
      541 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
      601 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccacag gctccgcccc
      661 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
      721 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
      781 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
      841 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
      901 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
      961 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     1021 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     1081 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     1141 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     1201 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     1261 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     1321 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     1381 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     1441 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     1501 gagggcttac catctggccc cagtgctgca atgataccgc gcgacccacg ctcaccggct
     1561 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     1621 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     1681 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     1741 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     1801 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     1861 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     1921 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     1981 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     2041 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     2101 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     2161 gcatctttta ctttcaccag cgtttctggg tg
//
