LOCUS       sequence-238067-         944 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_FR13_00076421
FEATURES             Location/Qualifiers
     source          1..944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(489..505)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(489..505)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(502..524)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    513..529
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(537..543,544..561,562..567))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    582..603
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(720..737)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttggtctcta atgggtggtg ctggtcaatc ttctgctgct gctgcttctg ctagacaagg
       61 tcaaggttct gttactgatg aatgtcatat ttttgttcat gaaaaagatg atccagttac
      121 tttgtttgct tatggtaaaa atgttattgg tcaaaatgct tttgttcaac cagttaaatt
      181 taataataat ccaaatgatc caagaattgt tccaatttct catttgagat tgaactctcc
      241 attgaatgat ttggattggg aattttctac tagagctgat gataaaaatc caactgaatg
      301 tattgctaaa attttgccaa atcaaaatgg ttgggttgaa ggtaaatatg ttttttggac
      361 tagaagaact actcaaaata aaattccaat ttggtatgaa aaaagattgg atgctccagg
      421 ttggttgtat gaatgagctt agagaccaag ttccatccca atggcgcgcc gagcttggct
      481 cgagcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
      541 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
      601 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
      661 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
      721 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
      781 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
      841 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
      901 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtca
//
