LOCUS       sequence-238070-         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_FR13_00081261
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(510..526)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(510..526)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(523..545)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    534..550
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(558..564,565..582,583..588))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    603..624
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(741..758)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttggtctcta atgagaccat ctggtccacc atctccagtt gctggtcatt ctaatactgc
       61 tgctagacca ccatctccag ttgctggttc ttctggtgct gctgctggtc caacttctcc
      121 aaaatctggt gaacaaaaac aacaagttcc agtttgtttg gttcaattgt tggaaggtga
      181 tttgttttgg aaattttata ctgctagacc aggtgaattg tatgcttttg ctattgaaaa
      241 tttgtctggt tcttttatta ctgctaaaga ttgtactatt aaagatgttt atggtaaaaa
      301 aattagaaga agatttactt tgaaatcttt tgctgcttct atggatttga tgactggtga
      361 attgactgtt catggtccag ctccagttgc taaaggttat tctggtgaaa ttcaagaagc
      421 ttatatgtct ttgtctactg gtagaaaatc tcattgagct tagagaccaa gttccatccc
      481 aatggcgcgc cgagcttggc tcgagcatgg tcatagctgt ttcctgtgtg aaattgttat
      541 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
      601 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
      661 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
      721 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
      781 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
      841 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
      901 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaa
//
