LOCUS       sequence-238076-        2171 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly DE part for Eck120033736 (164.6x-strength
            natural terminator; see DOI: 10.1038/nmeth.2515) - Please see 
            Supplemental Documents for annotated Genbank file.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2171)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: T2m
FEATURES             Location/Qualifiers
     source          1..2171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     20..38
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(42..85)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    88..109
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    202..222
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(228..245)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(399..987)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(479..498)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1158..1949,1950..2018))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1781..1800
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2019..2123)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 catgacatta acctataaaa ataggcgtat cacgaggcag aatttcagat aaaaaaaatc
       61 cttagctttc gctaaggatg atttctggaa ttcgcggccg cttctagaga ctagtgggtc
      121 tcaaggtcta acgcatgaga aagcccccgg aagatcacct tccgggggct tttttattgc
      181 gcccttgaga gcttagagac ctactagtag cggccgctgc aggcttcctc gctcactgac
      241 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
      301 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
      361 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccacaggct ccgcccccct
      421 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
      481 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
      541 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
      601 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
      661 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
      721 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
      781 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
      841 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
      901 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
      961 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     1021 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     1081 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     1141 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     1201 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     1261 ggcttaccat ctggccccag tgctgcaatg ataccgcgcg acccacgctc accggctcca
     1321 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     1381 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     1441 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     1501 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     1561 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     1621 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     1681 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     1741 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     1801 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     1861 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     1921 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     1981 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     2041 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     2101 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     2161 accattatta t
//
