LOCUS       sequence-238082-        2192 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly BC part for BCD20 (Medium expression 
            bi-cistronic RBS, engineered for downstream context-independence; 
            see https://doi.org/10.1038/nmeth.2404)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2192)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: U6m
FEATURES             Location/Qualifiers
     source          1..2192
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      481..1069
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     970..989
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1223..1240
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    1246..1266
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     misc_feature    1380..1401
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1404..1447
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(1451..1469)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1537..1641
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1642..1710,1711..2192,1..310)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1860..1879)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
       61 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
      121 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtcgc gcggtatcat
      181 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
      241 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
      301 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
      361 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
      421 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
      481 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
      541 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      601 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      661 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      721 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      781 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      841 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      901 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      961 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     1021 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctgtggaaaa acgccagcaa
     1081 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     1141 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     1201 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcctgca gcggccgcta
     1261 ctagtaggtc tctcattaga aactttcctc agcatgatta agatgtttca gtacgaaaat
     1321 tgctttcatt gttgatctcc tttttaagtg aacttgggcc cagtatgaga cccactagtc
     1381 tctagaagcg gccgcgaatt ccagaaatca tccttagcga aagctaagga ttttttttat
     1441 ctgaaattct gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     1501 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     1561 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     1621 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     1681 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     1741 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     1801 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     1861 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     1921 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     1981 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     2041 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     2101 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     2161 gacgagcgtg acaccacgat gcctgtagca at
//
