LOCUS       sequence-238107-        1355 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Chimeric ETS domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Albrecht AV, Kim HM, Poon GMK
  TITLE     Mapping interfacial hydration in ETS-family transcription factor 
            complexes with DNA: a chimeric approach.
  JOURNAL   Nucleic Acids Res. 2018 Nov 16;46(20):10577-10588. doi: 
            10.1093/nar/gky894.
  PUBMED    30295801
REFERENCE   2  (bases 1 to 1355)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2018-11-16- 16"; volume: "46"; issue: 
            "20"; pages: "10577-10588"
COMMENT     Sequence Label: pcDNA-PU.1-ETS
FEATURES             Location/Qualifiers
     source          1..1355
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     gap             2..14
                     /estimated_length=13
     primer_bind     68..87
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        68..86
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      116..125
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(557..574)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    563..790
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(924..943)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cnnnnnnnnn nnnnagcaga gctctctggc taactagaga acccactgct tactggctta
       61 tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgctac cggtcgccac
      121 catgggatcg aaaaaaaaga tccgccttta ccaattctta ctggaccttc tgcgctctgg
      181 agatatgaaa gactcaattt ggtgggttga caaagacaaa ggcacttttc aattcagttc
      241 aaagcataag gaagctctgg cccatcgttg gggaattcaa aaaggtaacc gcaagaaaat
      301 gacttaccag aaaatggcgc gtgccttgcg taactacgga aaaacagggg aggtgaagaa
      361 agtaaagaaa aaacttacat atcaattcag cggggaagtt ttaggacgcg gtggcttggc
      421 tgaacgccgt cacccacctc attgaaagct tggtaccgag ctcggatcca ctagtccagt
      481 gtggtggaat tctgcagata tccagcacag tggcggccgc tcgagtctag agggcccgtt
      541 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
      601 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
      661 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggn ttggggtggg
      721 ggcaggacag caagggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
      781 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
      841 ccctgtagcg gcgcattaag cgcggcgggn gntggtggtt acgcgcagcg tgaccgctac
      901 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
      961 cgccggcttt ccccgtcaag ctctaaatcg ggggctcccc tttagggttc cgatttagtg
     1021 ctttacggca cctcgacccc aaaaaacttg attaagggtg atgggttcac gtantggggc
     1081 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatanng
     1141 nactcttgtt ccaaactgga nnaannntca accctatcnn gntctattct ttngattnnn
     1201 aggnaatttt gccnaatttc ggcctattnn ttaaaaaann ancnngattt aacaaanttt
     1261 aacnnngaat taattnnnng gnaanggtnn nncnnttnng ggnnnngnaa angtcccnnn
     1321 gnttcccnnn cnnggccaaa ntnnnnnaan catgc
//
