LOCUS       sequence-238124-        5894 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a C. elegan codon optimized florescent protein 
            (CemNeptune2.5) under the intestinal specific promoter nhx-2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5894)
  AUTHORS   Thomas BJ, Wight IE, Chou WYY, Moreno M, Dawson Z, Homayouni A, 
            Huang H, Kim H, Jia H, Buland JR, Wambach JA, Cole FS, Pak SC, 
            Silverman GA, Luke CJ
  TITLE     CemOrange2 fusions facilitate multifluorophore subcellular imaging 
            in C. elegans.
  JOURNAL   PLoS One. 2019 Mar 26;14(3):e0214257. doi: 
            10.1371/journal.pone.0214257. eCollection 2019.
  PUBMED    30913273
REFERENCE   2  (bases 1 to 5894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-03-26"; volume: "14(3):e0214257. doi"; pages: " 
            10.1371/journal.pone.0214257. eCollection 2019"
COMMENT     Sequence Label: pJR2955
FEATURES             Location/Qualifiers
     source          1..5894
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3468..3490)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3590..3609
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3687..3705)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3773..3877
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3878..3946,3947..4738)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4096..4115)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4909..5497
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5398..5417
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5651..5668
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5785..5806
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5821..5826,5827..5844,5845..5851)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5859..5875
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5864..5886
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(5883..5894,1..5)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(5883..5894,1..5)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaccatga ttacgccaag cttccttcca agttattgac aagtattaac tgcacttctc
       61 ataaaaattt ttcaaataga tctatttagt ctggaatagg aatacttttg gcaattttct
      121 tgagaatcat agaagacgaa tttctgaact gctctttgtg aagccacgcc catatattgg
      181 catttttgta tccaattttt tgtagagtac ctgaatcatt gtcactacat ctgctaaaca
      241 atatatggaa gttctatgag aggtgaaagg taaatcatat aacaactgta ataacataga
      301 aatgattgta gtatatttcc aattaggctg agttgatctt attgaagagt ttgaatcata
      361 gcaatttttt cctcttttag tatttttcaa aacaaatttt cacaatgtta gatgtagtat
      421 ttagcattaa ggcatctgta atctcccaga aatgttttgg gaataaaacc ctttgactct
      481 ttggtccttt tttgctccaa ataatatctg attttaaccc gttctgatct actttttacc
      541 tttcgatcgt cacatccgcg tacacgtttc tcttgctctc aattgcttat ctttatggcc
      601 ttttccccca ctttttgtcg actatcatgc ggacgtggct tccttgctcc ctcccggttt
      661 ttttgaaaac acttgtgaat ttattttttg tggtcaaaat tacgaaacat ttgaattctc
      721 aatctattta tagaccgggt gtggtatgtt atgccatgtt tttgtcattg ccaaatgaac
      781 atcgatattt taattttctg aacttaccgt accccctgcg ttactatttt atttttaatt
      841 tgtataattt cattgctata atttgttgtc cttgaaacat tgaagatatc gaagaaataa
      901 tgacaaaaat cagatagaac aatttttcct actcatcaac tcacattttt ctattttctc
      961 ttgtgcgaaa aaaatagtat attgggaata tttatagtat tgcaactttt ctcttagttc
     1021 gcagagttgc aatactacta gatccctaat agaggaaata tggtgaccag ctttttgcaa
     1081 ttggaaagtt gggaaaaaag tatatatatt aatagactta cattcttgta gttcgtagaa
     1141 tttttcagga aatctgacac gcaagacttt ttcaaaaagt tttttttttt aatttggcaa
     1201 tctgccaaaa cgtttactga acattttgaa aacatctacc agtaccttta acacaacgaa
     1261 aaattgttct aagcaatatt aactgtgata attgatgtta agacctgaaa tttaatcaca
     1321 aatttcacct ggaaatgaca gtaatcggct tctaaatact gtcgacacaa gtgccatacc
     1381 taagattcca atcctattac catgtttccc aatttcattc tctttcgtat ctctatttct
     1441 cttctttatt atcaattact ttttatcagt tcttcgtttg ctttaacatc aagagcacat
     1501 agcgctctct cgtctctctc tcatgctctt tttgaacttt tacaaaaaac tatttccggt
     1561 tgttttgatt cttggaattt gaaataattt tcagtgatta aatctagagg atccccggga
     1621 ttggccaaag gacccaaagg tatgtttcga atgatactaa cataacatag aacattttca
     1681 ggaggaccct tggctagcct cgagaggcct ggtaccatgg gcggaaaaat ggtctccaag
     1741 ggagaggagc ttatcaagga gaacatgcac accaagctct acatggaggg aaccgtcaac
     1801 aaccaccact tcaagtgcac ccacgaggga gagggaaagc catacgaggg aacccaaacc
     1861 aaccgtatca aggtcgtcga gggaggacca ctcccattcg ccttcgacat cctcgccacc
     1921 tgcttcatgt acggatccaa ggtaagttta aacatatata tactaactaa ccctgattat
     1981 ttaaattttc agaccttcat caaccacacc caaggaatcc cagacttctt caagcaatcc
     2041 ttcccagagg gattcacctg ggagcgtgtc accacctacg aggacggagg agtcctcacc
     2101 gtcacccaag acacctccct ccaagacgga tgcctcatct acaacgtcaa gctccgtgga
     2161 gtcaacttcc catccaacgg accagtcatg caaaaggtaa gtttaaacag ttcggtacta
     2221 actaaccata catatttaaa ttttcagaag accctcggat gggaggcctc caccgagacc
     2281 ctctacccag ccgacggagg actagaggga cgttgcgaca tggccctcaa gctcgtcgga
     2341 ggaggacacc tccactgcaa cctcaagacc acctaccgtt ccaaggtaag tttaaacatg
     2401 attttactaa ctaactaatc tgatttaaat tttcagaagc cagccaagaa cctcaagatg
     2461 ccaggagtct acttcgtcga ccgtcgacta gagcgtatca aggaggccga caacgagacc
     2521 tacgtcgagc aacacgaggt cgccgtcgcc cgttactgcg acctcccatc caagctcgga
     2581 cacaagctca acggaatgga cgaactctac aagcttaacg gtcctaggag atctgaattc
     2641 ggcgcctaag agctccgcat cggccgctgt catcagatcg ccatctcgcg cccgtgcctc
     2701 tgacttctaa gtccaattac tcttcaacat ccctacatgc tctttctccc tgtgctccca
     2761 ccccctattt ttgttattat caaaaaactt ctcttaattt ctttgttttt tagcttcttt
     2821 taagtcacct ctaacaatga aattgtgtag attcaaaaat agaattaatt cgtaataaaa
     2881 agtcgaaaaa aattgtgctc cctcccccca ttaataataa ttctatccca aaatctacac
     2941 aatgttctgt gtacacttct tatgtttttt acttctgata aatttttttg aaacatcata
     3001 gaaaaaaccg cacacaaaat accttatcat atgttacgtt tcagtttatg accgcaattt
     3061 ttatttcttc gcacgtctgg gcctctcatg acgtcaaatc atgctcatcg tgaaaaagtt
     3121 ttggagtatt tttggaattt ttcaatcaag tgaaagttta tgaaattaat tttcctgctt
     3181 ttgctttttg gggtttcccc tattgtttgt caagatttcg aggacggcgt ttttcttgct
     3241 aaaatcacaa gtattgatga gcacgatgca agaaagatcg gaagaaggtt tgggtttgag
     3301 gctcagtgga aggtgagtag aagttgataa tttgaaagtg gagtagtgtc tatggggttt
     3361 ttgccttaaa tgacagaata cattcccaat ataccaaaca taactgtttc tactagtcgg
     3421 ccgtacgggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     3481 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     3541 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
     3601 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     3661 aaataccgca tcaggcggcc ttaagggcct cgtgatacgc ctatttttat aggttaatgt
     3721 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     3781 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     3841 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     3901 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     3961 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     4021 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     4081 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     4141 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     4201 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     4261 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     4321 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     4381 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     4441 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     4501 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     4561 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     4621 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     4681 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     4741 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     4801 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     4861 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     4921 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     4981 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     5041 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     5101 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     5161 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     5221 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     5281 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     5341 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     5401 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     5461 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     5521 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     5581 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     5641 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     5701 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     5761 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     5821 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     5881 cacaggaaac agct
//
