LOCUS       sequence-238127-       10249 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express M.EcoGII fused to CENPC with destabilization domain - 
            inducible.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10249)
  AUTHORS   Sobecki M, Souaid C, Boulay J, Guerineau V, Noordermeer D, Crabbe L
  TITLE     MadID, a Versatile Approach to Map Protein-DNA Interactions, 
            Highlights Telomere-Nuclear Envelope Contact Sites in Human Cells.
  JOURNAL   Cell Rep. 2018 Dec 4;25(10):2891-2903.e5. doi: 
            10.1016/j.celrep.2018.11.027.
  PUBMED    30517874
REFERENCE   2  (bases 1 to 10249)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2018.11"; journalName: "Cell Rep"; date: 
            "2018-12-4- 4"; volume: "25"; issue: "10"; pages: "2891-2903.e5"
COMMENT     Sequence Label: pRetroX-PTuner DD-M.EcoGII-v5-Centromeric prot...
FEATURES             Location/Qualifiers
     source          1..10249
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(428..444)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(428..444)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(441..463)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    452..468
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(476..482,483..500,501..506))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    521..542
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(659..676)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(830..1418)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(910..929)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1589..2380,2381..2449))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2450..2554)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2622..2640
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2678..2700)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2800..2819
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3013..3035
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3027..3044
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3028..3044
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             3177..3646
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    3711..4068
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     intron          4131..4334
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     4291..4311
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     exon            4335..4364
                     /label=EF-1-alpha exon
                     /note="truncated exon 2 from human EF-1-alpha, beginning 
                     with a GT-to-CC mutation that reduces splicing efficiency"
     CDS             join(4424..4426,4427..4747)
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD
                     /note="L106P mutant of FKBP12"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKPE"
     CDS             5915..5956
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    join(8806..9380,9381..9383,9384..9392)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8973..8990)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     9200..9219
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             9412..10008
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9412..9431)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9908..9928
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             join(10068..10249,1..412)
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
       61 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
      121 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
      181 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
      241 aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg
      301 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
      361 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca ttgaattcgt
      421 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
      481 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
      541 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
      601 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
      661 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
      721 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
      781 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
      841 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
      901 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
      961 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     1021 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     1081 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     1141 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     1201 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     1261 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     1321 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     1381 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     1441 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     1501 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     1561 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     1621 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     1681 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     1741 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     1801 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     1861 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     1921 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     1981 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     2041 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     2101 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     2161 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     2221 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     2281 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     2341 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     2401 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     2461 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     2521 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     2581 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     2641 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     2701 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     2761 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
     2821 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     2881 tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
     2941 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
     3001 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttatgtgaa
     3061 agaccccacc tgtaggtttg gcaagctagc ttaagtaacg ccattttgca aggcatggaa
     3121 aaatacataa ctgagaatag aaaagttcag atcaaggtca ggaacagatg gaacagctga
     3181 atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac
     3241 agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg
     3301 ctcagggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagagaa
     3361 ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact
     3421 aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccggg ctcaataaaa
     3481 gagcccacaa cccctcactc ggcgcgccag tcctccgatt gactgagtcg cccgggtacc
     3541 cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggcctcgct gttccttggg
     3601 agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcatttg ggggctcgtc
     3661 cgggatcggg agacccctgc ccagggacca ccgacccacc accgggaggt aagctggcca
     3721 gcaacttatc tgtgtctgtc cgattgtcta gtgtctatga ctgattttat gcgcctgcgt
     3781 cggtactagt tagctaacta gctctgtatc tggcggaccc gtggtggaac tgacgagttc
     3841 ggaacacccg gccgcaaccc tgggagacgt cccagggact tcgggggccg tttttgtggc
     3901 ccgacctgag tccaaaaatc ccgatcgttt tggactcttt ggtgcacccc ccttagagga
     3961 gggatatgtg gttctggtag gagacgagaa cctaaaacag ttcccgcctc cgtctgaatt
     4021 tttgctttcg gtttgggacc gaagccgcgc cgcgcgtctt gtctgctgca gcatcgttct
     4081 gtgttgtctc tgtctgactg tgtttctgta tttgtctgaa aatacgcgtg tcgagctttt
     4141 ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg
     4201 agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg
     4261 ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt
     4321 ttcttccatt tcagccgtcg tgaaaactac ccctaaaagc caaaaaaatc gaattcccaa
     4381 acttaagctt ggtaccgagc tcggatctca cgtgggcccg cggatgggag tgcaggtgga
     4441 aaccatctcc ccaggagacg ggcgcacctt ccccaagcgc ggccagacct gtgtggtgca
     4501 ctacaccggg atgcttgaag atggaaagaa agtcgattcc tcccgggaca gaaacaagcc
     4561 ctttaagttt atgctaggca agcaggaggt gatccgaggc tgggaagaag gggttgccca
     4621 gatgagtgtg ggtcagagag ccaaactgac tatatctcca gattatgcct atggtgccac
     4681 tgggcaccca ggcatcatcc caccacatgc cactctcgtc ttcgatgtgg agcttctaaa
     4741 accggaaccg cggccgcaga tctatcgatg gatcctatcc ggactcagat ctcgagctca
     4801 agcttcgaat tctgcagtcg acggtaccgc gggcagcgct gccaacctgc cggggatccg
     4861 tatgcttaat actgtaaaaa tatccagttg tgagttaatc aacgccgact gcctggaatt
     4921 tatccggtcg ttacccgaaa attctgttga cctgatagtc acggacccgc cgtactttaa
     4981 agtgaagccc gagggctggg ataaccagtg gaagggcgac gatgattacc tgaagtggct
     5041 ggaccagtgt ctggcgcagt tctggcgggt gctgaaacct gccggaagtc tttacctgtt
     5101 ctgtggccat cgcctggcat ctgacattga aatcatgatg cgtgaacgct tcagtgtgct
     5161 gaaccatatt atctgggcaa agccgtccgg acgctggaac gggtgcaaca aggaaagcct
     5221 gcgggcgtat ttccccgcca cagagcgcat tctgttcgcg gaacattatc aggggccgta
     5281 tcgcccgaaa gatgccgggt atgaggcgaa gggcagggca ctgaaacagc atgtgatggc
     5341 cccgctgatt gcttactttc gtgatgcgcg cgctgccctg gggataacgg caaaacagat
     5401 tgcagatgcc acaggaaaga aaaacatggt gccgcactgg ttcagtgcca gtcagtggca
     5461 gctaccgaac gaaagcgatt atctgaaatt acagtcgctg tttgcccggg tggcagaaga
     5521 gaaacatcag cgcggggaac tggaaaagcc acaccaccag ctggtcagca catacagtga
     5581 gctgaaccgg aagtatatgg aactgctgag tgaatataaa aatttgcggc ggtatttcgg
     5641 tgtgacggtg caggtgccgt acaccgatgt gtggacgtat aaaccggtgc agtactatcc
     5701 agggaaacat ccgtgcgaaa aaccggcaga aatgctgcag cagataatca gcgcaagtag
     5761 tcgtcctggt gatctggttg cggatttttt catggggtcg ggttcaacgg taaaagcggc
     5821 gatggcactg gggcgtcgtg cgattggtgt tgagctggag accggacgtt ttgagcagac
     5881 agtcagggaa gttcaggatt taatcgttcc atggggtaag cctatcccta accctctcct
     5941 cggtctcgat tctacgcgta ccggcatggc tgcgtccggt ctggatcatc tcaaaaatgg
     6001 ctacagaaga agattttgtc gaccttccag ggcacgtgac attaacacag agcaaggcca
     6061 gaatgttctg gaaatcttac aagactgttt tgaagaaaaa agtcttgcca atgattttag
     6121 tacaaattct acaaaatcag tgcctaattc aacacgcaaa ataaaagaca cttgtattca
     6181 gtcaccaagc aaagagtgcc agaaatcaca tccaaagtca gttccagttt cttcaaagaa
     6241 gaaagaagcc tctctacagt ttgttgtaga accaagtgaa gccacaaaca gatcagttca
     6301 ggcccatgaa gttcatcaga aaattctggc aactgatgtt agttccaaaa atacacctga
     6361 ctcgaaaaaa atatcaagta gaaacataaa tgatcatcac agtgaagctg atgaagaatt
     6421 ttacttatcc gttggctcac cttctgttct tttggatgca aaaacatctg tatcacaaaa
     6481 tgttattcca tctagtgccc aaaagagaga gacttacact tttgaaaatt cagtaaatat
     6541 gctgccttca agtacagagg tttcagttaa aaccaaaaaa aggttaaact ttgatgataa
     6601 agttatgtta aagaaaatag aaatagataa taaagtatca gatgaagagg ataaaacatc
     6661 agaaggacaa gaaagaaaac catcaggatc atctcagaat agaatacgag attcagaata
     6721 tgaaattcaa cgacaagcta aaaaaagttt ttcaacattg tttttagaaa cagtaaaacg
     6781 aaaaagtgaa tccagtccca ttgttaggca tgcggcaact gctccacctc attcgtgtcc
     6841 tcccgatgat acgaagttga tagaggatga atttataatt gatgagtcgg atcaaagttt
     6901 tgccagtaga tcttggatta caataccaag aaaggcaggg tctctgaaac aacgcacaat
     6961 atccccggct gagagcactg cactctttca aggtagaaag tcaagagaaa agcatcataa
     7021 tatattacct aagactttgg caaatgacaa acattcccat aaacctcacc cagtagagac
     7081 atctcagccc tctgataaaa cagtactgga tacaagttat gctttgatag gtgaaacagt
     7141 aaataattat agatctacaa aatatgaaat gtattccaag aatgcagaaa aaccatctag
     7201 aagcaaaagg actataaaac aaaaacagag aagaaaattc atggctaaac cagctgaaga
     7261 acagcttgat gtgggacagt ctaaagatga aaacatacat acatcacata ttacccaaga
     7321 cgaatttcaa agaaattcag acagaaatat ggaagagcat gaagagatgg gaaatgattg
     7381 tgtttccaaa aaacagatgc cacctgtggg aagcaagaaa agtagcacta gaaaagataa
     7441 ggaagaatct aaaaagaagc gcttttccag tgagtccaag aacaaacttg tacctgaaga
     7501 agtgacttca actgtcacga aaagtcgaag aatttccagg cgtccatctg attggtgggt
     7561 ggtaaaatca gaggagagtc ctgtttatag caattcttca gtaagaaatg aattaccaat
     7621 gcatcacaat agtagccgaa aatctactaa gaaaacaaat cagtcatcta agaatattag
     7681 gaaaaaaact attccactta aaaggcagaa gacagcaact aaaggcaacc aaagagtaca
     7741 gaagttttta aatgctgaag gttctggagg tatcgttggt catgatgaaa tttccagatg
     7801 ttcactgagt gagccattgg aaagtgatga ggcagacttg gctaagaaga aaaatcttga
     7861 ttgttctaga tctacaagaa gctcaaagaa tgaagataac attatgactg cacagaatgt
     7921 tcccctaaag cctcagacca gtggatatac atgtaatata ccaacagagt caaacttgga
     7981 ttctggagag cataagactt cagttttaga ggaaagtgga ccttccaggc tcaataataa
     8041 ttatttaatg tctggaaaga atgatgtgga tgatgaggaa gttcatggaa gttcagatga
     8101 ctcaaaacaa tctaaagtga taccaaagaa cagaatccat cacaaactag tattgccctc
     8161 caacacacca aatgttcgca ggaccaagag aacacgtttg aaacctttgg agtactggcg
     8221 aggagagcga atagattatc aaggaaggcc atcaggagga ttcgtgatta gtggagtact
     8281 atctccagac acaatatcgt ctaaaaggaa ggcaaaagaa aatattggaa aagtcaacaa
     8341 aaaatctaat aagaaaagga tctgtcttga taacgatgaa agaaagacta acttaatggt
     8401 aaatctaggt atacctcttg gagatccttt gcagccaacg agggtaaagg acccagaaac
     8461 aagagagatt attctcatgg atcttgtaag gccacaagat acatatcaat tttttgttaa
     8521 gcatggtgag ttgaaggtat acaagacatt ggatacaccc tttttttcta ctgggaaatt
     8581 gatattagga ccacaagaag aaaagggaaa gcagcatgtt ggccaggata tattggtttt
     8641 ttatgttaac tttggtgacc ttttgtgtac tttacatgaa acaccttata tattaagtac
     8701 tggggattcg ttctatgttc cttcaggtaa ctattataac atcaaaaatc tcctgaatga
     8761 ggaaagtgtt cttcttttta ctcagataaa aagatgacca tggcgcccct ctccctcccc
     8821 cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat
     8881 gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt
     8941 cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt
     9001 gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc
     9061 gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc
     9121 acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat
     9181 agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc
     9241 ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg
     9301 tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct
     9361 ttgaaaaaca cgatgataag cttgccacaa cccacaagga gacgaccttc catgaccgag
     9421 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc cccgggccgt acgcaccctc
     9481 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgacccgga ccgccacatc
     9541 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     9601 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
     9661 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     9721 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     9781 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     9841 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     9901 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     9961 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgccac cggtgcggct
    10021 tcgaggataa aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc
    10081 acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaaataca
    10141 taactgagaa tagagaagtt cagatcaagg tcaggaacag atggaacagc tgaatatggg
    10201 ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaa
//
