LOCUS       sequence-238129-        7193 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a C. elegan codon optimized florescent protein 
            (CemOrange2) fused to a early endosome localized gene (rab-5) under 
            the intestinal specific promoter nhx-2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7193)
  AUTHORS   Thomas BJ, Wight IE, Chou WYY, Moreno M, Dawson Z, Homayouni A, 
            Huang H, Kim H, Jia H, Buland JR, Wambach JA, Cole FS, Pak SC, 
            Silverman GA, Luke CJ
  TITLE     CemOrange2 fusions facilitate multifluorophore subcellular imaging 
            in C. elegans.
  JOURNAL   PLoS One. 2019 Mar 26;14(3):e0214257. doi: 
            10.1371/journal.pone.0214257. eCollection 2019.
  PUBMED    30913273
REFERENCE   2  (bases 1 to 7193)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-03-26"; volume: "14(3):e0214257. doi"; pages: " 
            10.1371/journal.pone.0214257. eCollection 2019"
COMMENT     Sequence Label: pBT3007
FEATURES             Location/Qualifiers
     source          1..7193
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(4767..4789)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4889..4908
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4986..5004)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5072..5176
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5177..5245,5246..6037)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5395..5414)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6208..6796
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6697..6716
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6950..6967
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7084..7105
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7120..7125,7126..7143,7144..7150)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7158..7174
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7163..7185
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(7182..7193,1..5)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(7182..7193,1..5)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaccatga ttacgccaag cttccttcca agttattgac aagtattaac tgcacttctc
       61 ataaaaattt ttcaaataga tctatttagt ctggaatagg aatacttttg gcaattttct
      121 tgagaatcat agaagacgaa tttctgaact gctctttgtg aagccacgcc catatattgg
      181 catttttgta tccaattttt tgtagagtac ctgaatcatt gtcactacat ctgctaaaca
      241 atatatggaa gttctatgag aggtgaaagg taaatcatat aacaactgta ataacataga
      301 aatgattgta gtatatttcc aattaggctg agttgatctt attgaagagt ttgaatcata
      361 gcaatttttt cctcttttag tatttttcaa aacaaatttt cacaatgtta gatgtagtat
      421 ttagcattaa ggcatctgta atctcccaga aatgttttgg gaataaaacc ctttgactct
      481 ttggtccttt tttgctccaa ataatatctg attttaaccc gttctgatct actttttacc
      541 tttcgatcgt cacatccgcg tacacgtttc tcttgctctc aattgcttat ctttatggcc
      601 ttttccccca ctttttgtcg actatcatgc ggacgtggct tccttgctcc ctcccggttt
      661 ttttgaaaac acttgtgaat ttattttttg tggtcaaaat tacgaaacat ttgaattctc
      721 aatctattta tagaccgggt gtggtatgtt atgccatgtt tttgtcattg ccaaatgaac
      781 atcgatattt taattttctg aacttaccgt accccctgcg ttactatttt atttttaatt
      841 tgtataattt cattgctata atttgttgtc cttgaaacat tgaagatatc gaagaaataa
      901 tgacaaaaat cagatagaac aatttttcct actcatcaac tcacattttt ctattttctc
      961 ttgtgcgaaa aaaatagtat attgggaata tttatagtat tgcaactttt ctcttagttc
     1021 gcagagttgc aatactacta gatccctaat agaggaaata tggtgaccag ctttttgcaa
     1081 ttggaaagtt gggaaaaaag tatatatatt aatagactta cattcttgta gttcgtagaa
     1141 tttttcagga aatctgacac gcaagacttt ttcaaaaagt tttttttttt aatttggcaa
     1201 tctgccaaaa cgtttactga acattttgaa aacatctacc agtaccttta acacaacgaa
     1261 aaattgttct aagcaatatt aactgtgata attgatgtta agacctgaaa tttaatcaca
     1321 aatttcacct ggaaatgaca gtaatcggct tctaaatact gtcgacacaa gtgccatacc
     1381 taagattcca atcctattac catgtttccc aatttcattc tctttcgtat ctctatttct
     1441 cttctttatt atcaattact ttttatcagt tcttcgtttg ctttaacatc aagagcacat
     1501 agcgctctct cgtctctctc tcatgctctt tttgaacttt tacaaaaaac tatttccggt
     1561 tgttttgatt cttggaattt gaaataattt tcagtgatta aatctagagg atccccggga
     1621 ttggccaaag gacccaaagg tatgtttcga atgatactaa cataacatag aacattttca
     1681 ggaggaccct tggctagcct cgagaggcct ggtaccatgg gcggaaaaat ggtctccaag
     1741 ggagaggaga acaacatggc catcatcaag gagttcatgc gtttcaaggt ccgtatggag
     1801 ggatccgtca acggacacga gttcgagatc gagggagagg gagagggacg tccatacgag
     1861 ggattccaaa ccgccaagct caaggtcacc aaggtaagtt taaacatata tatactaact
     1921 aaccctgatt atttaaattt tcagggagga ccactcccat tcgcctggga catcctctcc
     1981 ccacacttca cctacggatc caaggcctac gtcaagcacc cagccgacat cccagactac
     2041 ttcaagctct ccttcccaga gggattcaag tgggagcgtg tcatgaacta cgaggacgga
     2101 ggagtcgtca ccgtcaccca agactcctcc ctccaagacg gagagttcat ctacaaggta
     2161 agtttaaaca gttcggtact aactaaccat acatatttaa attttcaggt caagctccgt
     2221 ggaaccaact tcccatccga cggaccagtc atgcaaaaga agaccatggg atgggaggcc
     2281 tcctccgagc gtatgtaccc agaggacgga gccctcaagg gaaagatcaa gatgcgtctc
     2341 aagctcaagg taagtttaaa catgatttta ctaactaact aatctgattt aaattttcag
     2401 gacggaggac actacacctc cgaggtcaag accacctaca aggccaagaa gccagtccaa
     2461 ctcccaggag cctacatcgt cgacatcaag ctcgacatca cctcccacaa cgaggactac
     2521 accatcgtcg agcaatacga gcgtgccgag ggacgtcact ccaccggagg aatggacgaa
     2581 ctctacaagc ttaacggtcc taggagatct gaattcgccg cccgaaacgc aggaaccgcc
     2641 cggcctggag gtccaaacag aacgtgccag ttcaaactcg ttcttctcgg agaaagtgct
     2701 gtaggtgagt tgatactgaa aatggaaaaa accgctaaag gttggagtag aaaataaaag
     2761 caaacaagaa cagttttcca aaaaataaag atttttttat tctactccgt tctgtctgta
     2821 gcatatttcg cagttgctga aaaccgtcac aaaatttcga aattgcgttg atttcgaagt
     2881 ttaattcttc gtcttttgat tgtttatatg tttagaatta tgggtcatct gatagttgct
     2941 agcatatgac gtatatgctc tcttatttca cctaaacatc acagaaaact atcatttcag
     3001 gcaaatcatc tctcgtattg cgattcgtca agggacaatt tcacgagtat caggagtcga
     3061 ctataggtgc tgcgttcctc actcaaactg tgagtttttg aattttttgt gaaaatataa
     3121 caattttccc taatttgaac aaaaattttg actaaaatga aaaatctcga attttaataa
     3181 tatattgttt tttaataata taattgcgga gatttttgct cgaaaaacac ggattttata
     3241 ttgaaagacg taaaaacgtt aataattaag aattgaattt tcgaaatttc aggtgtgcct
     3301 tgacgatgca acaattaaat tcgaaatctg ggatactgca ggacaagaaa gatatcattc
     3361 attggcacca atgtactatc gaggagctca agcggctatt gttgtctacg atattaccaa
     3421 tcaagaatct ttccaaaaag cgaaaaattg ggtaaaagaa ctgcagcgac aggcatctcc
     3481 aaatattgtg atggcattgg ctggaaacaa ggctgatgtt gccaataagc gaactgttga
     3541 gtatgaagaa gctaatgctt atgctgagga caatgctctc ttgttcatgg agacatctgc
     3601 gaaggtacat tagaaaattt ggattttgac aataaaaaca tgttaacaga gtactgaaat
     3661 agatcaaaaa ctaaaattgt tattaaaaat aacaataaac tttgttttct gcgaagatag
     3721 ttttcgaact aatctccatt aaaaaatgaa tgagaaattt aagatttcca atttaaaatc
     3781 aattctctat ttatttaatt acagacctca atgaacgtga acgatatttt catggcaata
     3841 gctaaaaagt tgccaatcgg accagcacag ggtgaaccaa cgggaactgt cgatatgaac
     3901 caaccacaac agcaacaaaa gggttcatgc tgtaaataag gcgcctaaga gctccgcatc
     3961 ggccgctgtc atcagatcgc catctcgcgc ccgtgcctct gacttctaag tccaattact
     4021 cttcaacatc cctacatgct ctttctccct gtgctcccac cccctatttt tgttattatc
     4081 aaaaaacttc tcttaatttc tttgtttttt agcttctttt aagtcacctc taacaatgaa
     4141 attgtgtaga ttcaaaaata gaattaattc gtaataaaaa gtcgaaaaaa attgtgctcc
     4201 ctccccccat taataataat tctatcccaa aatctacaca atgttctgtg tacacttctt
     4261 atgtttttta cttctgataa atttttttga aacatcatag aaaaaaccgc acacaaaata
     4321 ccttatcata tgttacgttt cagtttatga ccgcaatttt tatttcttcg cacgtctggg
     4381 cctctcatga cgtcaaatca tgctcatcgt gaaaaagttt tggagtattt ttggaatttt
     4441 tcaatcaagt gaaagtttat gaaattaatt ttcctgcttt tgctttttgg ggtttcccct
     4501 attgtttgtc aagatttcga ggacggcgtt tttcttgcta aaatcacaag tattgatgag
     4561 cacgatgcaa gaaagatcgg aagaaggttt gggtttgagg ctcagtggaa ggtgagtaga
     4621 agttgataat ttgaaagtgg agtagtgtct atggggtttt tgccttaaat gacagaatac
     4681 attcccaata taccaaacat aactgtttct actagtcggc cgtacgggcc ctttcgtctc
     4741 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     4801 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     4861 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
     4921 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcggcct
     4981 taagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     5041 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     5101 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     5161 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     5221 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     5281 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     5341 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     5401 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     5461 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     5521 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     5581 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     5641 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     5701 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     5761 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     5821 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     5881 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     5941 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     6001 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     6061 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     6121 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     6181 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     6241 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     6301 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     6361 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     6421 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     6481 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     6541 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     6601 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     6661 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     6721 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     6781 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     6841 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     6901 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     6961 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     7021 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     7081 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     7141 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gct
//
