LOCUS       sequence-238133-       15168 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a C. elegan codon optimized florescent protein 
            (CemOrange2) fused to a lysosomal related organelle localized gene 
            (glo-1) under the intestinal specific promoter nhx-2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15168)
  AUTHORS   Thomas BJ, Wight IE, Chou WYY, Moreno M, Dawson Z, Homayouni A, 
            Huang H, Kim H, Jia H, Buland JR, Wambach JA, Cole FS, Pak SC, 
            Silverman GA, Luke CJ
  TITLE     CemOrange2 fusions facilitate multifluorophore subcellular imaging 
            in C. elegans.
  JOURNAL   PLoS One. 2019 Mar 26;14(3):e0214257. doi: 
            10.1371/journal.pone.0214257. eCollection 2019.
  PUBMED    30913273
REFERENCE   2  (bases 1 to 15168)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-03-26"; volume: "14(3):e0214257. doi"; pages: " 
            10.1371/journal.pone.0214257. eCollection 2019"
COMMENT     Sequence Label: pBT3038
FEATURES             Location/Qualifiers
     source          1..15168
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(12742..12764)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     12864..12883
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(12961..12979)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        13047..13151
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(13152..13220,13221..14012)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13370..13389)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      14183..14771
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14672..14691
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14925..14942
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    15059..15080
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(15095..15100,15101..15118,15119..15125)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15133..15149
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     15138..15160
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(15157..15168,1..5)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(15157..15168,1..5)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaccatga ttacgccaag cttccttcca agttattgac aagtattaac tgcacttctc
       61 ataaaaattt ttcaaataga tctatttagt ctggaatagg aatacttttg gcaattttct
      121 tgagaatcat agaagacgaa tttctgaact gctctttgtg aagccacgcc catatattgg
      181 catttttgta tccaattttt tgtagagtac ctgaatcatt gtcactacat ctgctaaaca
      241 atatatggaa gttctatgag aggtgaaagg taaatcatat aacaactgta ataacataga
      301 aatgattgta gtatatttcc aattaggctg agttgatctt attgaagagt ttgaatcata
      361 gcaatttttt cctcttttag tatttttcaa aacaaatttt cacaatgtta gatgtagtat
      421 ttagcattaa ggcatctgta atctcccaga aatgttttgg gaataaaacc ctttgactct
      481 ttggtccttt tttgctccaa ataatatctg attttaaccc gttctgatct actttttacc
      541 tttcgatcgt cacatccgcg tacacgtttc tcttgctctc aattgcttat ctttatggcc
      601 ttttccccca ctttttgtcg actatcatgc ggacgtggct tccttgctcc ctcccggttt
      661 ttttgaaaac acttgtgaat ttattttttg tggtcaaaat tacgaaacat ttgaattctc
      721 aatctattta tagaccgggt gtggtatgtt atgccatgtt tttgtcattg ccaaatgaac
      781 atcgatattt taattttctg aacttaccgt accccctgcg ttactatttt atttttaatt
      841 tgtataattt cattgctata atttgttgtc cttgaaacat tgaagatatc gaagaaataa
      901 tgacaaaaat cagatagaac aatttttcct actcatcaac tcacattttt ctattttctc
      961 ttgtgcgaaa aaaatagtat attgggaata tttatagtat tgcaactttt ctcttagttc
     1021 gcagagttgc aatactacta gatccctaat agaggaaata tggtgaccag ctttttgcaa
     1081 ttggaaagtt gggaaaaaag tatatatatt aatagactta cattcttgta gttcgtagaa
     1141 tttttcagga aatctgacac gcaagacttt ttcaaaaagt tttttttttt aatttggcaa
     1201 tctgccaaaa cgtttactga acattttgaa aacatctacc agtaccttta acacaacgaa
     1261 aaattgttct aagcaatatt aactgtgata attgatgtta agacctgaaa tttaatcaca
     1321 aatttcacct ggaaatgaca gtaatcggct tctaaatact gtcgacacaa gtgccatacc
     1381 taagattcca atcctattac catgtttccc aatttcattc tctttcgtat ctctatttct
     1441 cttctttatt atcaattact ttttatcagt tcttcgtttg ctttaacatc aagagcacat
     1501 agcgctctct cgtctctctc tcatgctctt tttgaacttt tacaaaaaac tatttccggt
     1561 tgttttgatt cttggaattt gaaataattt tcagtgatta aatctagagg atccccggga
     1621 ttggccaaag gacccaaagg tatgtttcga atgatactaa cataacatag aacattttca
     1681 ggaggaccct tggctagcaa aaatggcagc actcacaaat aacgataaaa tttcctacaa
     1741 agtgttggta attggtgatc caggtgtcgg taaaacatct attattcgtc gatttgttca
     1801 taatgtgttt tcgtcaaatt acaaaacgac agtaagtcaa ttttcctaac gtcagtttat
     1861 tcgaattgaa acatttttat tgcgaaatct tgagacaaga ttttttgcct atacatacat
     1921 gttaatttca cgaatggttt ccaactgctt tgttgttttc aattaaaaaa cttcatctgt
     1981 gaatagttgg gatggctgat actaattctg gtaatcaaag tcaatgcaaa ctaagacacc
     2041 aagtttttct tttcaatagt tgtgcttaaa attgacaatt catggttttg ttgtcattgg
     2101 attaataaaa tcgtttacgt acgaagtttc aataaatttt cttttactta tcgggattgg
     2161 tttctgccac tgaattctta ttgatataat tgaaaagaat cgtcagaaaa ttaaattcca
     2221 cctacttcag aagatctaaa actgtttatt aacgaaagtt ttctcagaag aacttatccc
     2281 aggatcatgt tttaagattt taagatattt atctattttc agatcggtgt tgactttgct
     2341 ctcaaaatcc tgcctgtcga cgagaacaca gttgttcatc ttcaaatctg ggatatttca
     2401 ggtgagcgaa aataaaattt ttatttatac ttttacatag aacagaaaat gattgttttc
     2461 aaatatttac acacaaacaa tttttggctt tgttgatttt tgtttctaaa tttggtgcaa
     2521 atttactggg attaggcgta caagtcatgt tactttaaag gaggatatat acgaatgggg
     2581 ttgaacacat tcctaataga tgtgtgtttt tgacgcagtt tcagaaattg agctgacaag
     2641 ttaaagcacc attttcctat tgggtattct ttttcaaaac aagaatatgg gttcaaactt
     2701 aatcgattga aagttgaggg agtgagcact tggggcggcg cggctggaaa aaacagacaa
     2761 agaagaggga aacaggtgtg aagcgatgtg gaaagggcaa tattgaggca gctaacagtt
     2821 tctagtgaag ttgattggct taatgcaaaa atagagttat tagttgttga agacatgtcc
     2881 aatttgtgtt tgtgacgccg atgattgtac cattctattt ggatcatctc caacatttgc
     2941 ggggctttga ccaggctgcc cacttccact tccagctagc gcatatccat aaaatctgaa
     3001 attaattagt tttattttag tagtttgagg tgatattcga atagaaagag tgataaacat
     3061 cacaattttg ttaagaattt taagtcaaca tttcatcaac gtcaacatta tcaagaaaac
     3121 gactaaaaat aaaaatttat actcaaatta ttgccctaga tgttgcaaat aaagtttgta
     3181 agagtggagg agtgcaatcc tatttgagac gcaatcctat acagggaaaa gtactaatag
     3241 ggaatacgaa agtttacgct tacgtgtttg cgccaggagt acttctctac aatcctaact
     3301 gattaaacaa tgtttttttc tcaaaatttt agggagtgct cttcaaatca acaaaatata
     3361 tatttccatt gtcctcttac ccagactcaa tttgataagg ttttccataa aatcgtttcg
     3421 aagtatgata ataaaatgga ttttcgtcag cactccagta aactgattgc tcttccattg
     3481 gctgaacgcc tcctccggcc tgcaatatat atcttcaatg cgagaattca gtaaaaagtt
     3541 accgggcttt gttgtgggtg gaagggtcgt gaaggtgtcc tcatatgtgt tccaactctc
     3601 aaagttccgc ttccaaactg ctcttctcca agcggagcga caacggtgct atctggtgag
     3661 gtagaccgat gatggagaat gtaagaggat ctgaactttg ttattctcta gatgtattaa
     3721 attttctggc cacacgtgtt ctatatgaat gcggttcgta tcccttccta ttcttgttat
     3781 ataaattatc aatatattca aaccttagcg agcaaatgat tgcgcaaaca gtgcagatga
     3841 cacacgcgag agctgcaccg gcggcgagaa tgatagcaac ctgatagatt gtttgctgtg
     3901 tttcagtcgt cggcaaaagg tcagatgctt gcaagtaccg ttgctgaaac aggttttcat
     3961 tggttttaga cttgagatat tgacaactca ccaaatagta aacattggta attgccgaag
     4021 ataccgtgaa caaagatatc gaagcaatga taatgtttaa gattgaagca gatgccaaga
     4081 gacttgggaa cggaatacag aggaattgaa caataaatac tgcaccagca agtggcccat
     4141 ttccaagtct gaagaacggc acttcgattg gaatttcatt gtatctccat tttccaagtt
     4201 caaacacaat gtaggttcca gtgataacca cggcataaaa cagtgttccc aatgagaata
     4261 atccatgaac tccatttgaa tgtcggtatg tcagatttgg ctgctttctg agttcagttc
     4321 cataaagata ggagccagca agtgatgtca atgcttcaag aagataaatc tgtaaataat
     4381 cactatttta acactatttt aatttgttcg aaaacgctat aagataaaaa ctcacaccag
     4441 cttgaatgga atacagtatg atctgcgcca caaatgttct gtcgtttccg tttcggtatg
     4501 cggggtaagc agccatttga tagataagat tggcagaggc acacattttt tcgactgaaa
     4561 ttccaaggca gacgacggag aggatgaatt ttgttgtgat catcacgctg aataatttaa
     4621 ttgagaaaat gtttctgttt taagataagc aacaaggatt aatttttgtg aatatacgtt
     4681 tgtcatctta gttgccactt cgtacctgta attattggaa gtcatcaatc cagttgtcat
     4741 gaaaagaacc gagacccaaa gaaggtcagc tcccaggaat tctcctctcc accaagtatc
     4801 caaaagtcca agaatacaca aagctagcat acatgcaccc cagaaaacat gaagaacatt
     4861 gaggactctg aaatttccaa cggttgtgtg gtaaatctcc acaatatgaa tcctaccctg
     4921 tttgaatttt gatgctcttg ttcacttcag tatgcattga gtggaagtca agatgtaggt
     4981 atgttccaac gataaccgct gacagtgtgg aaaggaaaaa tgcaattcca aagcatccca
     5041 aaagaactcc gtgagccaca accaaaatgt atcttggagc atcagttgag gtgattgatg
     5101 gatctaaagt ctatgtttag atgtatacgt tttatgctgt tgacatggtg tccaaaaatt
     5161 tgtggaaatt gaaaacatac acatatcaag tttcggtgta tatcttttac gtgtgcctct
     5221 tacgtatcac ttacctccga cctcgcgacc ctggtagtaa aatttcaagt catttttata
     5281 aaatggcatg gcgttgttat caactgtctt cagacaccat gtggctgcga cgacagctac
     5341 ggcgagagca acctatgcaa atattagggg tcattgagag tcaggataca cagattatat
     5401 cttcagctca cctttaagtt taaattctgt tgattctttc ctttggttgc ccagatgtcc
     5461 atggcaccgc taacaatgca caacatggcg gagatgtgat caagcccaat tttgatgtat
     5521 ttgtcttgcc cagcaaccta aacagtagtt tgtagggtgt aacatgaatc atctacaaag
     5581 caaaaaaaaa acaaaacagt acattttcgt gttcatactc aatgaataca cataatccga
     5641 ggattccaaa agcaagaatg aatttaatca aaccaattga ggccaatgtc ttttttgcgc
     5701 tgctcattgc aagttcttgt tctctctgaa tcatcgaaat ctagtatgaa ataattaatg
     5761 ttaggtgctg ggttagtgag tgtgatctta ctgattgtaa tggatatact ggaatagtga
     5821 caacaataat tttactgaga agaaagatgg cgaaaagtga gaacaatgaa gcaacggctc
     5881 cggacgctga aaatacataa agttttgcgt aaaaaactac atgtgtgttt accaatcata
     5941 agtgctgaaa tgacaattct tccagcgtag ttactgttct cagattgaaa ttcttcattg
     6001 aatccgtcag ctgactgaag ttgattcaag ttttcgccgg cttttacaac ctgaatcact
     6061 ttaaattatt ttatatgtta gtactttttc acttacccga tatgtggtga atccgtaaaa
     6121 tacggcagta gcaaatccga tcacaaaaga tactaaataa agtgacttgg ttacaatact
     6181 tccatgattc ctccacaacg agtaaaatcc aatgcatcca gtaactacat ggatgaagtt
     6241 cagtaaccga aagtcaatat ctcgatagtt atctaaacca acttttgaga attgacttcc
     6301 cgccaatcct actgctaata aaccagcagc aattagaaac ccattgcaaa tctaaaattt
     6361 cagaaaattc tgtgagtagt tatgtgttat ttacctgtat ttgacacaaa tatgccgagc
     6421 gggcgctcat attacaggat cttacttgct tttggcaact gataaaacaa gatgttcacc
     6481 cgggttccca cgctgaaaat tatatgaata taattgaaat aacactgttc aagttgaaac
     6541 tatatacacg aataattaat gcaaacgggg tcagtttcgc aaagagaaaa atggtgagag
     6601 agccgccaag gtgtgggacg agagaaggtg gaagggtgag agctcgtcga gcaaacacag
     6661 taggtgagat agatcaacaa aaatatgcaa cacaacagca gaacaacagc accgtcttct
     6721 ctcacttcca ttttcggaaa caagaattaa caaacctttt gtatgcgaga aaagcactag
     6781 gttgcaaaaa aataaagaca attttttcaa acattcaaat ggtacagagt aatagataaa
     6841 tacagtaagt tagggaaaat cagtttgtga acttaaaaaa aatgctaaca ataaaatata
     6901 gtaactcaaa acgatatatt ttcgagtttc aagcttgaat tgattaacgt agattatata
     6961 aatttcaggt acttcggtag tatacctgcc caaaaatatt ttcaatacgc ttaccattca
     7021 tttgttctga aaatgataat ttattcaaaa cgtgaaattg agatgcgttg ttaggcttaa
     7081 aaaaacacta atcctaatag taaacctttg agttttttaa tgtgaaaaac tgttacattc
     7141 ttgaagtagg tattctacac gagtactttc acaaacaaac agttccattt atgatggaac
     7201 attaactaca aattttcagt tttttttact ctgatttttt cagtttctaa tagcaagtac
     7261 tgaatttttg agagaaatat aagatttagt tcagctaaaa catgtcagaa ataataatat
     7321 gaaacaaata atatttatga tcgaatttag aacaaagtta aataattgtt gactgaaata
     7381 ttcgtaaaaa aataatttgt agacaattac aatacttcac ggcaatgtca attgctaagg
     7441 caaatgagga atctaatgaa taagggttta aagctttatg ggtgggcttt ttttttccag
     7501 acttgaaact ccgaaattca gggctctcaa gttcatgcgt taacattatc aatgtcaaaa
     7561 tgttttaaga gtgtaaaaga agaacacgac atgtaccgaa aaaaccagta gaccccattg
     7621 caatggggcg gtaaccactt caaacttaga aattatagaa caagagaggt aaagaaagag
     7681 agagagggag agacggtaag actcaaatca aggcggctgt cttttaacct aaatgcaaat
     7741 aatcaagctt gaagggggag ctcatggaaa aaaatgaggg aggaagattt tgataagcga
     7801 cctctctgtc ttttcttccc gcgtcgttcc gcgcctgttt gtatgcgacg atgtgcacac
     7861 attgtttacc agtggcggct gtgtaagttg ccacgcgggt aggtatgtgc agcaaaattt
     7921 gggaggtggg atctaccgag cggtgagcaa aaagtcgaaa agacgccgcc accagcgcta
     7981 tcacaggaaa aattggaaaa aatgtttctg gttttcaccg ggtgggcttt tagccctaat
     8041 gctattgtta agcagaaaca ctgcgcgaac ggcatcatta gattgcagga atgaatgaaa
     8101 ctatgcccta tttcttgcaa atatttagtt tcgacaaaaa tgtagttggg atgaaaaaaa
     8161 aagaatgaaa aatccaatga aaatcgaatc gttgctcatg tatgaggtgc ggaaaacaaa
     8221 tcgaattcgc aacgggaggt ttccattgta ttttgtggac acctttagtg tggagtttgt
     8281 ttttgaccct ccttccaccc ccaaattcgg ttagcgttgc acaggattgt gaaatgttaa
     8341 accggatggg cttcagagcg atgtgatttg tggcaataca attttgaaaa ctttaaactt
     8401 aacttccaaa ctgattattg atgtgctaca agtttttaat tgtaattttt agttaaaaaa
     8461 atggtccagg tttcattttc atttttgtcg atttttgaaa atctggcagt tttgtagtta
     8521 ttttgcaaca tatttcaaat tagcctgtag ttctgtacag ttgtaattaa caaatttatg
     8581 ctgaataatt aactaaatat tacctaccag ccgtggaccg cacctacggc gcagcccccg
     8641 acttttttga gaaaacacat tagatatacg gtgtctatcg cggacctcga tcgatgtctg
     8701 ctgcgcattt agcataaggt ttcccaaaat ttcgttgctc tgatcagaga tcaaactaca
     8761 tagggacaga aggacacgcg ctttatatat agtaaatgat aatatggaga agtggaaaca
     8821 atcagttgca atcttaagat ggtaaaacat aagtaacaat ggaacaaaga aaaacaagaa
     8881 ttttttttgc tcttattgta tcatgattac tctagagata aattgcaatt atcaaacagt
     8941 tacgattatt cgaaaaagaa gctcataact tcaaaatttt aaaaataatc tttgcgcttt
     9001 tcaaacctac ggaatctggt tcaacacagg tattcttttt gaaaaaagtg caaaactgtt
     9061 gagaaataag gaatcgggaa aggattgaac tgtgcattac acacgttccg gtaactgctc
     9121 gttgtgcgtg cagtctctgc cagactacgg aaaagatctt gtcttgggta tttgtgtgca
     9181 cattttgctc ccctttcccc ttcaacctcg atttccacgt agttttttgt ctctttgccc
     9241 tctctttttg atgctcatgt attatactaa agtaagtacg tcggcgcaag gatgagaggt
     9301 tgccgacgtt gcggaatccg gtccgcggct ggaaggtggc aagcgcacgc tcctgaatac
     9361 ttgtgcgtct gtctctgtgt gtttgtgcca acgttttgta taccagcaag aaattggaag
     9421 gaaacgggtg gggggaggag gagcggaaaa aactctccgc gcatagcttg tgcgtttctt
     9481 tctcacctca aaatcgttca caagttttac ctacacattg atcacagtgt gctaaataaa
     9541 agaagcaaat acaaatttca aaggcaacaa ctttgagatt gatgtttcac ggggaatact
     9601 gaacaatttt ttctagagtc ctctataaat aaacattatt tcatttacag gccaagaccg
     9661 atatggggtc atgaccagag tctattacaa agatgcacat gcagctatta tagttttgga
     9721 ttcaacaagg gaacgcacta tcgaaggtgc tttacgctgg aagactgatt tggatcaaaa
     9781 agtgacactg gccgatggat cgccagttcc agcaatttta cttgcaaaca aggtagtaga
     9841 gggtaaaaaa aaaagaatat gagttgtctg tttacagtgt gatatcgaca ataaacttgg
     9901 tgacgacaaa ctgtatgact tagaaacaaa caacggattt gtgggatcgt tccgcacttc
     9961 ggcaaaggaa agtgttggca ttgaggaggc gttcaaattt ttggcaaata cggtgtgtaa
    10021 aatatttttg caattagtga attgaactta attcgaaaat ttgatacgat tattttaaaa
    10081 ttaaaacgaa catgcggagc agaatttccc aaaaaacttc actcctaact tttctcataa
    10141 tttccggtca aagttttggc aaagtgccga aattttgaaa aatacgagca tttaaggaaa
    10201 tttaaagaaa tgtcacatgt ctcgacccct acaatcctct aatacaaata atttaaacaa
    10261 aattaaaaca taaaaaatgt cgaaaaaaaa tttttttttg gtcgacttcc aaaattatga
    10321 gtggcaaaaa ctgagtaatt gccacttttt gacagtaaat aaaaaatttt caaaaaattt
    10381 ttggaaagtt ttatcatgat atttcgtcat tttaggacca aaggagtggt ttttaacaat
    10441 ttccccattg gcgctactcc acctttaata cgttgtcaac tccattttgt agttatgttc
    10501 tgaaacattt caaaagctag aaaaatcact gatgcaaaga gagtgaacat gtgtgcaaat
    10561 tcagatcaca aatataaaat attctgcaat tgttttctta aactttgatg gccaatttgt
    10621 aggtaatttt caatgtgcat tgctgagttg ggatcaataa tttatctgtc ctcaacgatg
    10681 taatcactat tcttaatgta tctccgaagt ataaatacaa agcgatgaaa tccctctcat
    10741 tttggtccag tatttttata aactctgtcc agtatcaact tcaattaact caatttgtgc
    10801 tttaaaccaa taattgaatt tttaaaaaac tcgtattttc ccttgaagac ctttttttag
    10861 tcatttaaat tactaatgtt ttcaggtgat ctccactgaa caaggtggtc aatatgatgt
    10921 ccctttcatg aatcgtgaag gcaacgtgaa tcttgatgac aataccactc attctagata
    10981 cgactcgaaa tgttgcctcg agaggcctgg taccatgggc ggaaaaatgg tctccaaggg
    11041 agaggagaac aacatggcca tcatcaagga gttcatgcgt ttcaaggtcc gtatggaggg
    11101 atccgtcaac ggacacgagt tcgagatcga gggagaggga gagggacgtc catacgaggg
    11161 attccaaacc gccaagctca aggtcaccaa ggtaagttta aacatatata tactaactaa
    11221 ccctgattat ttaaattttc agggaggacc actcccattc gcctgggaca tcctctcccc
    11281 acacttcacc tacggatcca aggcctacgt caagcaccca gccgacatcc cagactactt
    11341 caagctctcc ttcccagagg gattcaagtg ggagcgtgtc atgaactacg aggacggagg
    11401 agtcgtcacc gtcacccaag actcctccct ccaagacgga gagttcatct acaaggtaag
    11461 tttaaacagt tcggtactaa ctaaccatac atatttaaat tttcaggtca agctccgtgg
    11521 aaccaacttc ccatccgacg gaccagtcat gcaaaagaag accatgggat gggaggcctc
    11581 ctccgagcgt atgtacccag aggacggagc cctcaaggga aagatcaaga tgcgtctcaa
    11641 gctcaaggta agtttaaaca tgattttact aactaactaa tctgatttaa attttcagga
    11701 cggaggacac tacacctccg aggtcaagac cacctacaag gccaagaagc cagtccaact
    11761 cccaggagcc tacatcgtcg acatcaagct cgacatcacc tcccacaacg aggactacac
    11821 catcgtcgag caatacgagc gtgccgaggg acgtcactcc accggaggaa tggacgaact
    11881 ctacaagctt aacggtccta ggagatctga attcggcgcc taagagctcc gcatcggccg
    11941 ctgtcatcag atcgccatct cgcgcccgtg cctctgactt ctaagtccaa ttactcttca
    12001 acatccctac atgctctttc tccctgtgct cccaccccct atttttgtta ttatcaaaaa
    12061 acttctctta atttctttgt tttttagctt cttttaagtc acctctaaca atgaaattgt
    12121 gtagattcaa aaatagaatt aattcgtaat aaaaagtcga aaaaaattgt gctccctccc
    12181 cccattaata ataattctat cccaaaatct acacaatgtt ctgtgtacac ttcttatgtt
    12241 ttttacttct gataaatttt tttgaaacat catagaaaaa accgcacaca aaatacctta
    12301 tcatatgtta cgtttcagtt tatgaccgca atttttattt cttcgcacgt ctgggcctct
    12361 catgacgtca aatcatgctc atcgtgaaaa agttttggag tatttttgga atttttcaat
    12421 caagtgaaag tttatgaaat taattttcct gcttttgctt tttggggttt cccctattgt
    12481 ttgtcaagat ttcgaggacg gcgtttttct tgctaaaatc acaagtattg atgagcacga
    12541 tgcaagaaag atcggaagaa ggtttgggtt tgaggctcag tggaaggtga gtagaagttg
    12601 ataatttgaa agtggagtag tgtctatggg gtttttgcct taaatgacag aatacattcc
    12661 caatatacca aacataactg tttctactag tcggccgtac gggccctttc gtctcgcgcg
    12721 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
    12781 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
    12841 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
    12901 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc ggccttaagg
    12961 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
    13021 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
    13081 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
    13141 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
    13201 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
    13261 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
    13321 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
    13381 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
    13441 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
    13501 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
    13561 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
    13621 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
    13681 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
    13741 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
    13801 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
    13861 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
    13921 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
    13981 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
    14041 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
    14101 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
    14161 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
    14221 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
    14281 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
    14341 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
    14401 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
    14461 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
    14521 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
    14581 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
    14641 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
    14701 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
    14761 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
    14821 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
    14881 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
    14941 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
    15001 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
    15061 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
    15121 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagct
//
