LOCUS       sequence-238134-         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_FR13_00107801
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(666..682)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(666..682)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(679..701)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    690..706
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(714..720,721..738,739..744))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    759..780
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(897..914)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttggtctcta atgactccag ctgctgctgg ttcttctgaa gatatgccaa ttgaaacttt
       61 gcaacaattg tctggtagaa aaccaactaa aaatagaaaa ttgttggatc aatatgctca
      121 agatatgcaa agagatccac caccaagagg tatgattcca ttgccatctc atgttgatgc
      181 ttttcaagtt ccagaagcta ctcaaatgta tgctccaggt tatgatttga ataaagctcc
      241 atgtagaatt gttatttata gaccaactaa tccaattggt ggtactggtg aagaatattt
      301 tatgggtaat gttgctccag gtggtgttgg tagatttgat gaatatattt gtaaagcttc
      361 taaagattgt ttgagaccaa cttgttttga tatgccattt ggttttagat attttggtag
      421 acatatgaga tatgaacatg ttttggattt tcaatatggt agaattggta ttaaagaagc
      481 tgaagataga tatcaaaaat tgcaagctga agaacaagct agaagagaac aagatagaag
      541 agaagctgaa ttgaatccac cacaatctaa accaaaatct tggtgggctc cattgactcc
      601 atggcaacaa tgagcttaga gaccaagttc catcccaatg gcgcgccgag cttggctcga
      661 gcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
      721 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
      781 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
      841 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
      901 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
      961 gcggtaatac ggttatcc
//
