LOCUS       sequence-238138-         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_FR13_00110721
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(306..322)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(306..322)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(319..341)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    330..346
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(354..360,361..378,379..384))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    399..420
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(537..554)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(788..807)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttggtctcta atggacccaa gacattatag atctcaagtt gattctaatt tgggtggtca
       61 tttgaataga agagctcatt tggatggttt gtctgaatgt ccagatactc aagctgttca
      121 aaatgctatt catggtttga aaactagaca tgctattaaa aatccagatt cttatgctat
      181 ttttgctcaa tgtgctagaa atggtgattg tgaagatgaa tctgattatt attctgattc
      241 tgattctgat tgagcttaga gaccaagttc catcccaatg gcgcgccgag cttggctcga
      301 gcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
      361 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
      421 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
      481 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
      541 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
      601 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
      661 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
      721 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
      781 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
      841 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
      901 catagctcac gctgt
//
