LOCUS       sequence-238150-         965 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_FR13_00128431
FEATURES             Location/Qualifiers
     source          1..965
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(430..446)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(430..446)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(443..465)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    454..470
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(478..484,485..502,503..508))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    523..544
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(661..678)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(912..931)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cttggtctct aatgggtaga ggttcttatt tgactgttaa accatctgaa ttggataaac
       61 atattgctca attggaagaa tatttgagaa atccaccatg taaagttact atttatgctc
      121 caccaccaaa aaaaggtaga actcaattga gaactgaaat gggttttgct tctgctggta
      181 aaaatgaaga tgttccagtt aaaattgctt ctactggtca tacttatcaa tgtagaactg
      241 atgctacttg tgaagaacca gtttgtcatg gtttgccaaa tggttggact tatgatggtg
      301 tttatatgca tacttttact gaattgtctt tggaacaatt gaaagctcaa aaagctgctc
      361 aaacttctag acattgagct tagagaccaa gttccatccc aatggcgcgc cgagcttggc
      421 tcgagcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
      481 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
      541 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
      601 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
      661 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
      721 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
      781 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
      841 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
      901 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
      961 tccga
//
