LOCUS       sequence-238153-         981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_FR13_00144271
FEATURES             Location/Qualifiers
     source          1..981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(396..412)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(396..412)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(409..431)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    420..436
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(444..450,451..468,469..474))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    489..510
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(627..644)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(878..897)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttggtctcta agctcattta gcacaaccaa taaaagcatt ataaacattt ttttgagaat
       61 cagttctttt acaagtacca ccttgaacaa aagaagtaac agttctacca acattaaaaa
      121 tttcaattct tggtggatca caaccacctc tattatcatt tctagcttga gaacattgtg
      181 gagatggatg attaattttt ttacctttag atctagcagt aataatttta acacaagaaa
      241 cacaataaga gttcaaaccc aaacctttac aagattcaga agaatctgga gtaccttgat
      301 cacaacacaa ttcaccagta cctggattaa tattaatttc aaccattaga gaccaagttc
      361 catcccaatg gcgcgccgag cttggctcga gcatggtcat agctgtttcc tgtgtgaaat
      421 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
      481 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
      541 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
      601 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
      661 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
      721 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
      781 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
      841 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
      901 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
      961 tttctccctt cgggaagcgt g
//
