LOCUS       sequence-238160-         678 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tuning expression of cytosolic Ovalbumin coupled to EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Michaels YS, Barnkob MB, Barbosa H, Baeumler TA, Thompson MK, Andre 
            V, Colin-York H, Fritzsche M, Gileadi U, Sheppard HM, Knapp DJHF, 
            Milne TA, Cerundolo V, Fulga TA
  TITLE     Precise tuning of gene expression levels in mammalian cells.
  JOURNAL   Nat Commun. 2019 Feb 18;10(1):818. doi: 10.1038/s41467-019-08777-y.
  PUBMED    30778069
REFERENCE   2  (bases 1 to 678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-02-18"; volume: "10(1):818. doi"; pages: " 
            10.1038/s41467-019-08777-y"
COMMENT     Sequence Label: OVA-miSFIT-2xPerfect
FEATURES             Location/Qualifiers
     source          1..678
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(156..175)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     503..524
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
       61 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
      121 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
      181 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
      241 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
      301 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
      361 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
      421 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
      481 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
      541 ctctcggcat ggacgagctg tacaaggaat tctaactaga gctcgcacgt gagtgctagc
      601 ctacctgcac tgtaagcact ttgtatctac ctgcactgta agcactttga ccggtactag
      661 cttagtcatg caccgggc
//
