LOCUS       sequence-238161-         651 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tuning expression of cytosolic Ovalbumin coupled to EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Michaels YS, Barnkob MB, Barbosa H, Baeumler TA, Thompson MK, Andre 
            V, Colin-York H, Fritzsche M, Gileadi U, Sheppard HM, Knapp DJHF, 
            Milne TA, Cerundolo V, Fulga TA
  TITLE     Precise tuning of gene expression levels in mammalian cells.
  JOURNAL   Nat Commun. 2019 Feb 18;10(1):818. doi: 10.1038/s41467-019-08777-y.
  PUBMED    30778069
REFERENCE   2  (bases 1 to 651)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-02-18"; volume: "10(1):818. doi"; pages: " 
            10.1038/s41467-019-08777-y"
COMMENT     Sequence Label: OVA-miSFIT-1xPerfect
FEATURES             Location/Qualifiers
     source          1..651
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(155..174)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     502..523
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
       61 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
      121 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
      181 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
      241 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
      301 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
      361 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
      421 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
      481 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
      541 tctcggcatg gacgagctgt acaaggaatt ctaactagag ctcgcacgtg agtgctagcc
      601 tacctgcact gtaagcactt tgaccggtac tagcttagtc atgcaccggg c
//
