LOCUS       sequence-238187-        7353 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a C. elegan codon optimized florescent protein 
            (CemOrange2) fused to a ER localized gene (tram-1) under the 
            intestinal specific promoter nhx-2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7353)
  AUTHORS   Thomas BJ, Wight IE, Chou WYY, Moreno M, Dawson Z, Homayouni A, 
            Huang H, Kim H, Jia H, Buland JR, Wambach JA, Cole FS, Pak SC, 
            Silverman GA, Luke CJ
  TITLE     CemOrange2 fusions facilitate multifluorophore subcellular imaging 
            in C. elegans.
  JOURNAL   PLoS One. 2019 Mar 26;14(3):e0214257. doi: 
            10.1371/journal.pone.0214257. eCollection 2019.
  PUBMED    30913273
REFERENCE   2  (bases 1 to 7353)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-03-26"; volume: "14(3):e0214257. doi"; pages: " 
            10.1371/journal.pone.0214257. eCollection 2019"
COMMENT     Sequence Label: pBT3000
FEATURES             Location/Qualifiers
     source          1..7353
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(4927..4949)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5049..5068
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5146..5164)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5232..5336
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5337..5405,5406..6197)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5555..5574)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6368..6956
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6857..6876
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7110..7127
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7244..7265
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7280..7285,7286..7303,7304..7310)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7318..7334
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7323..7345
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(7342..7353,1..5)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(7342..7353,1..5)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaccatga ttacgccaag cttccttcca agttattgac aagtattaac tgcacttctc
       61 ataaaaattt ttcaaataga tctatttagt ctggaatagg aatacttttg gcaattttct
      121 tgagaatcat agaagacgaa tttctgaact gctctttgtg aagccacgcc catatattgg
      181 catttttgta tccaattttt tgtagagtac ctgaatcatt gtcactacat ctgctaaaca
      241 atatatggaa gttctatgag aggtgaaagg taaatcatat aacaactgta ataacataga
      301 aatgattgta gtatatttcc aattaggctg agttgatctt attgaagagt ttgaatcata
      361 gcaatttttt cctcttttag tatttttcaa aacaaatttt cacaatgtta gatgtagtat
      421 ttagcattaa ggcatctgta atctcccaga aatgttttgg gaataaaacc ctttgactct
      481 ttggtccttt tttgctccaa ataatatctg attttaaccc gttctgatct actttttacc
      541 tttcgatcgt cacatccgcg tacacgtttc tcttgctctc aattgcttat ctttatggcc
      601 ttttccccca ctttttgtcg actatcatgc ggacgtggct tccttgctcc ctcccggttt
      661 ttttgaaaac acttgtgaat ttattttttg tggtcaaaat tacgaaacat ttgaattctc
      721 aatctattta tagaccgggt gtggtatgtt atgccatgtt tttgtcattg ccaaatgaac
      781 atcgatattt taattttctg aacttaccgt accccctgcg ttactatttt atttttaatt
      841 tgtataattt cattgctata atttgttgtc cttgaaacat tgaagatatc gaagaaataa
      901 tgacaaaaat cagatagaac aatttttcct actcatcaac tcacattttt ctattttctc
      961 ttgtgcgaaa aaaatagtat attgggaata tttatagtat tgcaactttt ctcttagttc
     1021 gcagagttgc aatactacta gatccctaat agaggaaata tggtgaccag ctttttgcaa
     1081 ttggaaagtt gggaaaaaag tatatatatt aatagactta cattcttgta gttcgtagaa
     1141 tttttcagga aatctgacac gcaagacttt ttcaaaaagt tttttttttt aatttggcaa
     1201 tctgccaaaa cgtttactga acattttgaa aacatctacc agtaccttta acacaacgaa
     1261 aaattgttct aagcaatatt aactgtgata attgatgtta agacctgaaa tttaatcaca
     1321 aatttcacct ggaaatgaca gtaatcggct tctaaatact gtcgacacaa gtgccatacc
     1381 taagattcca atcctattac catgtttccc aatttcattc tctttcgtat ctctatttct
     1441 cttctttatt atcaattact ttttatcagt tcttcgtttg ctttaacatc aagagcacat
     1501 agcgctctct cgtctctctc tcatgctctt tttgaacttt tacaaaaaac tatttccggt
     1561 tgttttgatt cttggaattt gaaataattt tcagtgatta aatctagagg atccccggga
     1621 ttggccaaag gacccaaagg tatgtttcga atgatactaa cataacatag aacattttca
     1681 ggaggaccct tggctagcct cgagaggcct ggtaccatgg gcggaaaaat ggtctccaag
     1741 ggagaggaga acaacatggc catcatcaag gagttcatgc gtttcaaggt ccgtatggag
     1801 ggatccgtca acggacacga gttcgagatc gagggagagg gagagggacg tccatacgag
     1861 ggattccaaa ccgccaagct caaggtcacc aaggtaagtt taaacatata tatactaact
     1921 aaccctgatt atttaaattt tcagggagga ccactcccat tcgcctggga catcctctcc
     1981 ccacacttca cctacggatc caaggcctac gtcaagcacc cagccgacat cccagactac
     2041 ttcaagctct ccttcccaga gggattcaag tgggagcgtg tcatgaacta cgaggacgga
     2101 ggagtcgtca ccgtcaccca agactcctcc ctccaagacg gagagttcat ctacaaggta
     2161 agtttaaaca gttcggtact aactaaccat acatatttaa attttcaggt caagctccgt
     2221 ggaaccaact tcccatccga cggaccagtc atgcaaaaga agaccatggg atgggaggcc
     2281 tcctccgagc gtatgtaccc agaggacgga gccctcaagg gaaagatcaa gatgcgtctc
     2341 aagctcaagg taagtttaaa catgatttta ctaactaact aatctgattt aaattttcag
     2401 gacggaggac actacacctc cgaggtcaag accacctaca aggccaagaa gccagtccaa
     2461 ctcccaggag cctacatcgt cgacatcaag ctcgacatca cctcccacaa cgaggactac
     2521 accatcgtcg agcaatacga gcgtgccgag ggacgtcact ccaccggagg aatggacgaa
     2581 ctctacaagc ttaacggtcc taggagatct gaattcggcg ccggaggagg aggatccgtt
     2641 aagccgcaag gagggtcgaa agcgtcgaaa aaacctcaac caccaatttt atcacatgag
     2701 ttcattatcc aaaatcatgg tgatattatg tcgtgtgtcg tgatggtgtt cattgtcgga
     2761 ttgatgttcc cattgactca ttcactctcc tcgctcttca ttgctcctca atacaacggt
     2821 acttatactg tggctgtaga acaaggccag gaacgcgaag ttcatggata tttgagcgga
     2881 attttggatc ttcctgccat tttcttctac tcggtttgct ggatcgtcgt gcatgctgtt
     2941 gtgcaagagt atggtctcga caaaatctcg aaaaaggtaa atttttcgtc aacataacat
     3001 ttatttttta aatgttttta tagtttcaat ttaaaacaat tcaaattaga aagaagtaga
     3061 acctctcggt gatgtgtatc tactgaacaa aaaatttaac tttaattaaa aaaaaacatt
     3121 ctcataattg cagactcact tgtcaaaggt ctccaccttc aaatttggcg agtcattcca
     3181 ccagatgttt ttcactgttt attctatcgc ccatgcgttt tacatcgttt ctgagcggct
     3241 cgaagatttc tcagaagtta aaaggtattc gtaaaatcca aatagtttta aacttttccc
     3301 acctagtttc agtgtttggc tcggataccc gacagagcat cgtgtaatgt ctgcagcata
     3361 caagctttac ttcatcttcc aaatttccta ctggattcat caattcccag agttctatct
     3421 gcaaaagctg aagcgcgacg agattcgcca aaagagtgtt caggctattc ttcatattgc
     3481 cttcatatca atcgcatact tcttcaattt cacacgtgtt ggattggcac ttattactct
     3541 cgaatacatt actcaattga ttttccatat tgcacgtttc gcacattttg ttggacgcaa
     3601 agggttgtct gacccagcct tcaagctgtt caacggttca ttcgttcttg ttcgtcttgg
     3661 atcaatcatt atcgccgtca tgactttctg gtacgggctt cgtcaagcag aatctccgtt
     3721 tgtggatatt tctgctggaa acttcaatac agcagttatc cggctcaatg ttcttcttgc
     3781 cgttgttctg ctccaactct tccttttgta ttcatttgtt gtattccata tgggacgatt
     3841 ccgtgagagc aacgctaaga aagaaaagaa gaagtctgct gctgctgctg cagctgttcc
     3901 taaaaaggag aaaaaaagac aagactctgt gagtttttac ctgcattttc tgagctcaag
     3961 ttttaatatg ttattcatat cctgattata aaagtagact atagaataag gcaaaacatc
     4021 aaagtgcttc taaaaaaaag aaacattttc cgtttcaata accttcaata ttttcaggag
     4081 agcgattcga agaagaaaaa ttaactaaga gctccgcatc ggccgctgtc atcagatcgc
     4141 catctcgcgc ccgtgcctct gacttctaag tccaattact cttcaacatc cctacatgct
     4201 ctttctccct gtgctcccac cccctatttt tgttattatc aaaaaacttc tcttaatttc
     4261 tttgtttttt agcttctttt aagtcacctc taacaatgaa attgtgtaga ttcaaaaata
     4321 gaattaattc gtaataaaaa gtcgaaaaaa attgtgctcc ctccccccat taataataat
     4381 tctatcccaa aatctacaca atgttctgtg tacacttctt atgtttttta cttctgataa
     4441 atttttttga aacatcatag aaaaaaccgc acacaaaata ccttatcata tgttacgttt
     4501 cagtttatga ccgcaatttt tatttcttcg cacgtctggg cctctcatga cgtcaaatca
     4561 tgctcatcgt gaaaaagttt tggagtattt ttggaatttt tcaatcaagt gaaagtttat
     4621 gaaattaatt ttcctgcttt tgctttttgg ggtttcccct attgtttgtc aagatttcga
     4681 ggacggcgtt tttcttgcta aaatcacaag tattgatgag cacgatgcaa gaaagatcgg
     4741 aagaaggttt gggtttgagg ctcagtggaa ggtgagtaga agttgataat ttgaaagtgg
     4801 agtagtgtct atggggtttt tgccttaaat gacagaatac attcccaata taccaaacat
     4861 aactgtttct actagtcggc cgtacgggcc ctttcgtctc gcgcgtttcg gtgatgacgg
     4921 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     4981 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
     5041 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
     5101 gcacagatgc gtaaggagaa aataccgcat caggcggcct taagggcctc gtgatacgcc
     5161 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     5221 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     5281 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     5341 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     5401 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     5461 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     5521 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     5581 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     5641 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     5701 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     5761 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     5821 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     5881 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     5941 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     6001 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     6061 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     6121 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     6181 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     6241 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     6301 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     6361 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     6421 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     6481 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     6541 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     6601 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     6661 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     6721 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     6781 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     6841 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     6901 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     6961 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     7021 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     7081 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     7141 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     7201 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     7261 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     7321 tgagcggata acaatttcac acaggaaaca gct
//
