LOCUS       sequence-238190-         393 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses SpyDock to bind SpyTag non-covalently for affinity 
            purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Khairil Anuar INA, Banerjee A, Keeble AH, Carella A, Nikov GI, 
            Howarth M
  TITLE     Spy
  JOURNAL   Nat Commun. 2019 Apr 15;10(1):1734. doi: 10.1038/s41467-019-09678-w.
  PUBMED    30988307
REFERENCE   2  (bases 1 to 393)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-04-15"; volume: "10(1):1734. doi"; pages: " 
            10.1038/s41467-019-09678-w"
COMMENT     Sequence Label: SpyDock
FEATURES             Location/Qualifiers
     source          1..393
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             37..375
                     /codon_start=1
                     /product="improved SpyCatcher variant that reacts with 
                     SpyTag003 at a rate approaching the diffusion limit (Keeble
                     et al., 2019)"
                     /label=SpyCatcher003
                     /translation="VTTLSGLSGEQGPSGDMTTEEDSATHIKFSKRDEDGRELAGATME
                     LRDCSGKTISTWISDGHVKDFYLYPGKYTFVATAAPDGYEVATPITFTVNEDGQVTVNG
                     EATEGDAHT"
     CDS             37..375
                     /codon_start=1
                     /product="improved SpyCatcher variant that shows rapid 
                     reaction with SpyTag002 (Keeble et al., 2017)"
                     /label=SpyCatcher002
                     /translation="VTTLSGLSGEQGPSGDMTTEEDSATHIKFSKRDEDGRELAGATME
                     LRDCSGKTISTWISDGHVKDFYLYPGKYTFVATAAPDGYEVATPITFTVNEDGQVTVNG
                     EATEGDAHT"
ORIGIN
        1 atgtcgtact accatcacca tcaccatcac gccatggtaa ccaccttatc aggtttatca
       61 ggtgagcaag gtccgtccgg tgatatgaca actgaagaag atagtgctac ccatattaaa
      121 ttctcaaaac gtgatgagga cggccgtgag ttagctggtg caactatgga gttgcgtgat
      181 tgctctggta aaactattag tacatggatt tcagatggac atgtgaagga tttctacctg
      241 tatccaggaa aatatacatt tgtcgcgacc gcagcaccag acggttatga ggtagcaact
      301 ccaattacct ttacagttaa tgaggacggt caggttactg taaatggcga agcaactgaa
      361 ggtgacgctc atactggatc cagtggtagc taa
//
