LOCUS       sequence-238197-         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_GY11_00054591
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(393..409)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(393..409)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(406..428)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    417..433
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(441..447,448..465,466..471))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    486..507
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(624..641)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttggtctcta agctcaagaa ccaccagaaa ctctattatt ttttggttta tcattaataa
       61 attgaacttc tttagaacct tctctagaca atctaataaa ccaattttct gggattctac
      121 cagcaccttt aactttagta cctttacatt caccatcaat atcaatagca aaatcagttt
      181 ttctagctct agcttcaata gtaatttcag ctctttgacc tggatcaata aaagaagaca
      241 aaaatggttc agtataccaa atatgcaaaa cttcaaattt acatctatat ggatgcaatt
      301 gttgatcatt agatctaact tcttgcaaat taactggacc cattagagac caagttccat
      361 cccaatggcg cgccgagctt ggctcgagca tggtcatagc tgtttcctgt gtgaaattgt
      421 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
      481 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
      541 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
      601 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
      661 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
      721 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
      781 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
      841 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat acc
//
