LOCUS       sequence-238198-        6975 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a C. elegan codon optimized florescent protein 
            (CemOrange2) fused to a plasma membrane localized gene (aqp-1) under
            the intestinal specific promoter nhx-2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6975)
  AUTHORS   Thomas BJ, Wight IE, Chou WYY, Moreno M, Dawson Z, Homayouni A, 
            Huang H, Kim H, Jia H, Buland JR, Wambach JA, Cole FS, Pak SC, 
            Silverman GA, Luke CJ
  TITLE     CemOrange2 fusions facilitate multifluorophore subcellular imaging 
            in C. elegans.
  JOURNAL   PLoS One. 2019 Mar 26;14(3):e0214257. doi: 
            10.1371/journal.pone.0214257. eCollection 2019.
  PUBMED    30913273
REFERENCE   2  (bases 1 to 6975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-03-26"; volume: "14(3):e0214257. doi"; pages: " 
            10.1371/journal.pone.0214257. eCollection 2019"
COMMENT     Sequence Label: pBT3102
FEATURES             Location/Qualifiers
     source          1..6975
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(4549..4571)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4671..4690
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4768..4786)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4854..4958
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4959..5027,5028..5819)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5177..5196)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5990..6578
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6479..6498
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6732..6749
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6866..6887
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6902..6907,6908..6925,6926..6932)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6940..6956
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6945..6967
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(6964..6975,1..5)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(6964..6975,1..5)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaccatga ttacgccaag cttccttcca agttattgac aagtattaac tgcacttctc
       61 ataaaaattt ttcaaataga tctatttagt ctggaatagg aatacttttg gcaattttct
      121 tgagaatcat agaagacgaa tttctgaact gctctttgtg aagccacgcc catatattgg
      181 catttttgta tccaattttt tgtagagtac ctgaatcatt gtcactacat ctgctaaaca
      241 atatatggaa gttctatgag aggtgaaagg taaatcatat aacaactgta ataacataga
      301 aatgattgta gtatatttcc aattaggctg agttgatctt attgaagagt ttgaatcata
      361 gcaatttttt cctcttttag tatttttcaa aacaaatttt cacaatgtta gatgtagtat
      421 ttagcattaa ggcatctgta atctcccaga aatgttttgg gaataaaacc ctttgactct
      481 ttggtccttt tttgctccaa ataatatctg attttaaccc gttctgatct actttttacc
      541 tttcgatcgt cacatccgcg tacacgtttc tcttgctctc aattgcttat ctttatggcc
      601 ttttccccca ctttttgtcg actatcatgc ggacgtggct tccttgctcc ctcccggttt
      661 ttttgaaaac acttgtgaat ttattttttg tggtcaaaat tacgaaacat ttgaattctc
      721 aatctattta tagaccgggt gtggtatgtt atgccatgtt tttgtcattg ccaaatgaac
      781 atcgatattt taattttctg aacttaccgt accccctgcg ttactatttt atttttaatt
      841 tgtataattt cattgctata atttgttgtc cttgaaacat tgaagatatc gaagaaataa
      901 tgacaaaaat cagatagaac aatttttcct actcatcaac tcacattttt ctattttctc
      961 ttgtgcgaaa aaaatagtat attgggaata tttatagtat tgcaactttt ctcttagttc
     1021 gcagagttgc aatactacta gatccctaat agaggaaata tggtgaccag ctttttgcaa
     1081 ttggaaagtt gggaaaaaag tatatatatt aatagactta cattcttgta gttcgtagaa
     1141 tttttcagga aatctgacac gcaagacttt ttcaaaaagt tttttttttt aatttggcaa
     1201 tctgccaaaa cgtttactga acattttgaa aacatctacc agtaccttta acacaacgaa
     1261 aaattgttct aagcaatatt aactgtgata attgatgtta agacctgaaa tttaatcaca
     1321 aatttcacct ggaaatgaca gtaatcggct tctaaatact gtcgacacaa gtgccatacc
     1381 taagattcca atcctattac catgtttccc aatttcattc tctttcgtat ctctatttct
     1441 cttctttatt atcaattact ttttatcagt tcttcgtttg ctttaacatc aagagcacat
     1501 agcgctctct cgtctctctc tcatgctctt tttgaacttt tacaaaaaac tatttccggt
     1561 tgttttgatt cttggaattt gaaataattt tcagtgatta aatctagagg atccccggga
     1621 ttggccaaag gacccaaagg tatgtttcga atgatactaa cataacatag aacattttca
     1681 ggaggaccct tggctagcaa aaatgacggc cgaggaagat actttgcctg aaaggctgag
     1741 gtttcacgga gttcacacaa atattttggc aaggaacctc atcgccgagt tttttggaac
     1801 attccttctt tgtgttagtt ttaagtttct tgaatagtaa tttgaaaatt tgattttttt
     1861 ttagtttatc ggtctttcaa ttgttttcca attccatgct ggcggtggaa agacaactga
     1921 atggattgga gttaacatag gatggggatt tgcaattatg tttgcagtta tggcaacagc
     1981 taggatgtca ggtaatattt ctgtgaatca aatcttctgc tcaattttct ttttatttta
     2041 agaactttta tcaacatctt tcaggtggtc atctcaatcc agctgtttcc cttctacttt
     2101 ggtctcttgg tcatctcaaa cttgcttggg tgccactgta cgcaattgct caaaccgctg
     2161 gagctttcgt ggcttctctt ggaatgtact cttattacta tggtatccta cttattctcc
     2221 ctgttgaaat tattttgaaa ttttcagaac aattcaatgc atttgatgga ggtaatcgca
     2281 caatcctcgg agctactgga acagctggtt gctttgccag ttatccatct ccaaatcttg
     2341 gagtatgggg tccatacatt gatcagtgcg tcggtaccgg agttctcgca tacttcctct
     2401 gcgttgttat tgatgagaga aatcaaattc caaaaatttg gcatccaatg ttcttcggat
     2461 tcttggtaat gatgatcgga actggatttg gaatgaacat tggatatcca attaatccag
     2521 caagagatct cggaccacga ctcttcagtt attttatcta tggtccaggt gttttccagt
     2581 tagttcacag tgtcaattca caaatatctc atataatgat ttcagctctc cataccctaa
     2641 ctattggcta gctccagcca ttgctccatt tgttggtgct cttgtcggtg gatggttcta
     2701 tcatttctcc cttggaatgc acaatcctga catcgaagaa gccgacgata tttttgttca
     2761 acaaccacca aaatctgtag agcaacaaaa attgcttcaa gctctcgaga ggcctggtac
     2821 catgggcgga aaaatggtct ccaagggaga ggagaacaac atggccatca tcaaggagtt
     2881 catgcgtttc aaggtccgta tggagggatc cgtcaacgga cacgagttcg agatcgaggg
     2941 agagggagag ggacgtccat acgagggatt ccaaaccgcc aagctcaagg tcaccaaggt
     3001 aagtttaaac atatatatac taactaaccc tgattattta aattttcagg gaggaccact
     3061 cccattcgcc tgggacatcc tctccccaca cttcacctac ggatccaagg cctacgtcaa
     3121 gcacccagcc gacatcccag actacttcaa gctctccttc ccagagggat tcaagtggga
     3181 gcgtgtcatg aactacgagg acggaggagt cgtcaccgtc acccaagact cctccctcca
     3241 agacggagag ttcatctaca aggtaagttt aaacagttcg gtactaacta accatacata
     3301 tttaaatttt caggtcaagc tccgtggaac caacttccca tccgacggac cagtcatgca
     3361 aaagaagacc atgggatggg aggcctcctc cgagcgtatg tacccagagg acggagccct
     3421 caagggaaag atcaagatgc gtctcaagct caaggtaagt ttaaacatga ttttactaac
     3481 taactaatct gatttaaatt ttcaggacgg aggacactac acctccgagg tcaagaccac
     3541 ctacaaggcc aagaagccag tccaactccc aggagcctac atcgtcgaca tcaagctcga
     3601 catcacctcc cacaacgagg actacaccat cgtcgagcaa tacgagcgtg ccgagggacg
     3661 tcactccacc ggaggaatgg acgaactcta caagcttaac ggtcctagga gatctgaatt
     3721 cggcgcctaa gagctccgca tcggccgctg tcatcagatc gccatctcgc gcccgtgcct
     3781 ctgacttcta agtccaatta ctcttcaaca tccctacatg ctctttctcc ctgtgctccc
     3841 accccctatt tttgttatta tcaaaaaact tctcttaatt tctttgtttt ttagcttctt
     3901 ttaagtcacc tctaacaatg aaattgtgta gattcaaaaa tagaattaat tcgtaataaa
     3961 aagtcgaaaa aaattgtgct ccctcccccc attaataata attctatccc aaaatctaca
     4021 caatgttctg tgtacacttc ttatgttttt tacttctgat aaattttttt gaaacatcat
     4081 agaaaaaacc gcacacaaaa taccttatca tatgttacgt ttcagtttat gaccgcaatt
     4141 tttatttctt cgcacgtctg ggcctctcat gacgtcaaat catgctcatc gtgaaaaagt
     4201 tttggagtat ttttggaatt tttcaatcaa gtgaaagttt atgaaattaa ttttcctgct
     4261 tttgcttttt ggggtttccc ctattgtttg tcaagatttc gaggacggcg tttttcttgc
     4321 taaaatcaca agtattgatg agcacgatgc aagaaagatc ggaagaaggt ttgggtttga
     4381 ggctcagtgg aaggtgagta gaagttgata atttgaaagt ggagtagtgt ctatggggtt
     4441 tttgccttaa atgacagaat acattcccaa tataccaaac ataactgttt ctactagtcg
     4501 gccgtacggg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     4561 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     4621 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
     4681 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
     4741 aaaataccgc atcaggcggc cttaagggcc tcgtgatacg cctattttta taggttaatg
     4801 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     4861 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     4921 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     4981 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     5041 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     5101 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     5161 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     5221 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     5281 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     5341 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     5401 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     5461 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     5521 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     5581 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     5641 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     5701 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     5761 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     5821 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     5881 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     5941 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6001 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6061 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6121 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6181 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6241 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6301 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     6361 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     6421 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     6481 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     6541 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     6601 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     6661 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     6721 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     6781 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     6841 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     6901 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     6961 acacaggaaa cagct
//
