LOCUS       sequence-238199-         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_GY11_00060011
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(446..462)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(446..462)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(459..481)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    470..486
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(494..500,501..518,519..524))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    539..560
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(677..694)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 acttggtctc taatggctcc aactccatct tctccaagag gtgctactca tgttggtaaa
       61 ttggaagctt ataataaaat gcaaccaaga ggtgatccaa ctgctggttt gtcttctaga
      121 gctccaaaag ctggtgatca tattgctgat tttgatcaat tgccaggtgg tgaatataga
      181 ggtcaagatg aagctaaagc tgctaaattg gaagctagag gtggtccagg tttggaatat
      241 agagttggtg atccagttgc ttctgctttg ttgagagata cttctttgca aacttctaga
      301 tctggtaata tgattagatt ttggagacca gaaggtgtta cttatcaagg tccacaagct
      361 aaaatgcata ctgcttcttt tatggctgtt tgagcttaga gaccaagttc catcccaatg
      421 gcgcgccgag cttggctcga gcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
      481 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
      541 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
      601 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
      661 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
      721 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
      781 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
      841 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
      901 agaggtggcg aaacccgaca ggactataaa
//
