LOCUS       sequence-238201-         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_GY11_00067931
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(399..415)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(399..415)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(412..434)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    423..439
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(447..453,454..471,472..477))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    492..513
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(630..647)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(881..900)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttggtctcta agctcaagca acaactttat aatatttaat aatagtacca tcatcatctt
       61 tagaatcagc atgaaattga catctatttt tatcaattgg attacattct ttatcccatt
      121 tacaaccagc aaattcatat ggaacaaatt tccaagaata tttagatcta ccaactcttt
      181 caacttgagt accaatcaaa gtatcaccat caaacaacaa tttataaaaa ataccaaatt
      241 cacaacctgg aactctttta ttaaaagcat tcaatctaaa atcagtaatt ggtctaactt
      301 caatcaattt cattggaact tctggtggtg gtttttttgg tggacccatt agagaccaag
      361 ttccatccca atggcgcgcc gagcttggct cgagcatggt catagctgtt tcctgtgtga
      421 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
      481 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
      541 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
      601 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
      661 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
      721 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
      781 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
      841 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
      901 cctggaagct ccctcgtgcg ctctcctgtt cc
//
